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  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...biology. In our case, we selected a project in a field with 30 years of basic research standing behind ...are well understood. Additional work has also identified and characterized mechanisms and components of...

  3. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...During this project, authors are asked to add an identifier (a bit like a GenBank ID) to all antibodies, ...a paper is received by other researchers in the field. Dos and don’ts when publishing a scientific manuscript...

  4. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...protocol, it is feasible to produce genetically modified cells in less than a month's time. Addgene: What...Musunuru: The CRISPR/Cas9 system is easier to use as testified by the fact that the TALEN kit includes 834 plasmids...

  5. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...part in rolling circle DNA amplification. The amplified DNA segment is labeled with fluorescent probes...protein levels in situ, the Schumann lab used a modified version of PLA where one antibody was directed...

  6. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...Mitalipov and his colleagues have achieved, and how the field will move forward from this work. Experiments & ...paper represents a large step forward in the CRISPR field, it’s important to remember that the study hasn’...

  7. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...correct insert Sequence the regions that have been amplified by PCR, putting special attention in the areas...try a different cloning strategy. Once you've identified potential clones after the screening, sequence...

  10. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...the lack of an Fc region. The lack of Fc also simplifies analyses when used in structural studies and ...1986) Two embryonic, tissue-specific molecules identified by a double-label immunofluorescence technique...

  11. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...how much faster it would be for an expert in the field to read this paper than it would be for an undergrad...the length of your average PhD to achieve!) Each field has its own framework: a particular way of organizing...

  12. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...discern the proteins in your assay.  Narrowing the field After working your way through the above steps, ...publication. Talk with your peers. If someone in your field has used the antibody before you will not only have...

  13. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ... While secondary antibodies are useful signal amplifiers, the flexibility of their use varies across applications...each protein of interest, this gel-based method identifies proteins solely by weight and size, relying on...

  15. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...processes. Every plasmid we receive is rigorously verified before becoming available to the community. This...overnight cultures with 15% glycerol), as opposed to purified DNA or colony sequencing. This means that we are...

  16. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...preexisting. This was previously debated in the cancer field and was difficult to determine using NGS because...barcodes in the population (all barcodes can be amplified using the same sets of forward and reverse primers...

  18. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...synapse from one cell to another, it must replicate. Modified Rabies Virus: Converting a transsynaptic neuronal...first-order connections to the starter cell, RABV was modified to only infect user-defined starter cell types...

  20. 6 Tips for Grant Writing

    Type
    Blog Post
    ...is to collaborate with specialists in different fields that can provide different insight into an area...your work and possibly a new perspective on the field. 3. Highlight your network and your collaborators...
Showing: 321 - 340 of 665 results