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We narrowed to 856 results for: HA

Showing: 321 - 340 of 856 results

  1. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...in previous animal studies suggested that fasting has a positive effect on cancer treatment (Ref 1). As... mutations and clinical responses. This platform has the potential to become an open-source pipeline and...of the plasmids on her poster is pTomo-Ras which has been deposited at Addgene was used to produce the...

  2. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...useful research materials and information. Addgene has been successful in helping thousands of sharing scientists...depositors and partners. The Addgene Scientist Team has been developing expertise in answering questions ...from plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative...

  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... capsid? A: It is our understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore...but reproducible success remains elusive.  There has also been a lot of effort in immunomodulation or ...AAV re-administration but I do not think the field has settled on ‘best practices’ for model organisms.  ...

  4. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR...Detailed figure legend here.     The Trimmer Lab has continued to work to make the sequences of monoclonal...collection and make them publicly available. The lab has also expanded their recombinant affinity reagent ...

  5. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...partnership was fruitful and a first version of the study has just been uploaded to the preprint repository BioRxiv... platform; while they are a minority, their help has been essential to push certain projects. The idea...year on, our biggest lesson running the platform has been the realization that there is a great untapped...

  6. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...makes a good mentoring program. If your organization has a mentoring program, consider tapping into it and... have going on locally. Your university probably has an alumni directory. Alumni who enter their information... own peer mentoring circle. This type of program has many advantages and I am seeing a lot of success ...

  7. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...I was excited to see that Addgene has recently added a new feature for depositing labs to its website...anytime. The CSV is a rich source of information and has data on the date, material, requesting PI & organization...

  8. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...Request, an expansion of our viral vectors service, has officially launched! As of today, you can request... on.  Expanding our viral vector service Addgene has offered in-stock viral vectors since 2016, with the...

  9. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...continue their careers in the United States. There has been a growing shift away from employers sponsoring...that the researcher is working under in the lab or has won, awards, media reports, and membership in groups...that you are a classically trained ballerina who has performed at Lincoln Center. Your case should only...only support tenure track professors. Casseus Law has seen that research institutions are willing to sponsor... your current research goal is and why your work has impacted your field (i.e. finding vaccines for deadly...The platinum standard for an expert is someone who has cited your work. Immediate steps that you can take...

  10. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...trimers, tetramers). Since then, multimerization has been observed to occur in many different kinds of...sequences. In some specific cases, multimerization has been observed to occur more frequently in plasmids... Ausubel, 1976). In fact, if your plasmid sample has a mix of different-sized multimers, you may even ...~3.6 kb and ~1.1 kb (lane 3). Uncut DNA (lane 2) has a band at <3 kb, which corresponds to the supercoiled...multimers is capillary gel electrophoresis (CGE), which has been used in plasmid vaccine and gene therapy development...

  11. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...changes, and the addition of protein tags. It also has the flexibility to modify the E. coli chromosome,...recombineering system The lambda red recombineering system has three components (Figure 1): 1) Exo, 2) Beta, and... in the center of the sequence. Since lambda red has a higher recombination frequency when the lagging...activating MMR: 1) use a strain of bacteria that has key MMR proteins knocked out or 2) specially design...recombinant clones If a antibiotic resistance gene has been inserted, recombinants can first be selected...

  12. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...The Rett Syndrome Research Trust (RSRT) has an ambitious goal of curing Rett Syndrome and Addgene is ....  As a non-degenerative, monogenic disease that has shown reversibility of symptoms in animal models,...neuronal subtypes of the hippocampus. This technology has formed the basis of the Rett syndrome program being....1138389 Le TTH, Tran NT, Dao TML, Nguyen DD, Do HD, Ha TL, Kühn R, Nguyen TL, Rajewsky K, Chu VT (2019) ...

  13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...within the human genome. Another variant, xCas9 3.7 has 7 mutations found in the REC2, REC3, and PAM interacting...CRISPR toolbox The isolation of novel CRISPR proteins has and will continue to dramatically increase the number...additional targeting flexibility. Cas13a (formerly C2c2) has been adapted for targeted RNA cleavage in mammalian... MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved Cas9 variants with ...

  14. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...never went away. While he has frequent bouts with Impostor Syndrome, Joe has found his people through ... match people within similar fields (ASM Microbe has eight tracks), something we didn’t have enough people...notebook paper, despite the fact that it was gray. He has worked in molecular and microbiology for the last...

  15. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...as a scientist has been building your creativity all along. In my own career, my team has used these techniques...and instead building an environment where everyone has their ideas heard.  So how can we assure that all...continue advocating for your idea. Our product team has implemented a policy of never rejecting an idea simply...

  16. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...right corner.      Figure 2: This flow plot has two populations that are B+ and one population that...Figure 3 above, until the population of interest has been successfully identified and isolated.  Following...from Boix et al., 2018, uses quadrants to gate and has numbers representing the percentage of cells in each...2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and increased ...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...epitope (GKPIPNPLLGLDST) has been used as a tag since 1992 (Hanke et al., 1992), and has proven itself a versatile...like PROTAC targeted protein degraders. But CRBN has been difficult to study in vitro, requiring either...Cas9 system By Aryana Khosravani Deborah Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9 system...

  18. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...optical pooled CRISPR screening, Paul Blainey’s lab has developed a multiplexing approach to Cas9-based pooled... stabilize the 3’ end of the pegRNA. As research has just begun with PE7, it will be exciting to see future...conferred several new improvements. The new variant has increased signal-to-noise ratio and better ability...limitations of traditional AAVs, the Becirovic Lab has developed REVeRT, for gene delivery using reconstitution...

  20. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ... undermine critical research infrastructure that has taken decades to establish.These funding decisions...Together Throughout history, the scientific community has demonstrated remarkable resilience and creativity...
Showing: 321 - 340 of 856 results