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We narrowed to 771 results for: LIS

Showing: 321 - 340 of 771 results

  1. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...audience’s eye and draw people in. That can be accomplished with an exciting title and attractive figures...in less than a couple of minutes. How does one accomplish all of that? What follows are some tips to help...logical progression of your work, and you can accomplish this by employing a neat and organized format...

  2. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus...Travers M, Chen Y, Garcia N, Whiteman MB, McKay LGA, Lelis FJN, Habibi S, Cai Y, Rennick LJ, Duprex WP, McCarthy...

  3. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...has developed a new, plasmid-based biosensor to visualise changes in histone methylation in living cells...substrate (the histone-derived peptide) which can localise the construct specifically to the histone protein...in the middle of a FRET pair, which is used to visualise the results. When methylation occurs at the histone-derived...

  4. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...more likely to view your proposals favorably. 5) Listen to your supervisor’s viewpoint and brainstorm about...with your supervisor, prioritize your action item list and set some approximate timelines. Be sure that...conflicts if you communicate your ideas assertively, listen to his or her viewpoint, and discuss options for...

  5. PhD Applications After COVID

    Type
    Blog Post
    ... for you and your goals. Making a List of Schools Creating a list of schools is one of the first steps.... With virtual options, you can narrow down your list of schools by meeting with professors - a/k/a Principle...

  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...as dot blot, enzyme-linked immunosorbent assay (ELISA) (Grimm et al., 1999), quantitative PCR (qPCR) (...blog post.  We generally do not recommend using ELISA-based titering assays, since those will quantify...Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production of recombinant...

  8. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...also been a great place to reach out to local establishments such as museums, professional organizations... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...

  9. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...techniques. More and more CRISPR tools are being published each month, making CRISPR a great choice for your...to consult the literature to see if anyone has published a protocol that would work for your system. Addgene... are a few plasmids at Addgene that have been published as CRISPR testing tools: Traffic Light Reporter...

  10. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...cistron will start with the Proline.  The table below lists the four common 2A peptides employed by scientists...to express two or more genes, some of which are listed below. We should note that these vectors are primarily...gene or genes of interest. Some of the plasmids listed in the table above (and their related plasmids)...

  11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    .... What the BioBricks Foundation is seeking to accomplish is to make the engineering of biology much easier...like this conversation we are having now is in English, and then there are languages to communicate with.... The question is who owns languages? Who owns English? Well, everybody does; it's free to use and that...

  12. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...some accountants running our math tables!  Be realistic about what you can teach  5-10 year olds in a ...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated...them had imagined for a scientist. I think that establishing this history helped demonstrate that a) I was...

  13. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...Funded by the NSF (with Co-PIs Jessica Bell, Lisa Gentile, Ellis Bell, and Amy Springer), this group now connects...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

  16. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...or monster.com, where you’ll find a variety of listings. Post your CV and make it visible, which will ...in a recruiter giving you a call. Many companies enlist the assistance of recruiters for identifying appropriate...know very well; always ask for information and establish a connection with that person before ever asking...

  17. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...people or discussing science, it is a great way to establish yourself as a thought leader and have real conversations...on subreddits (r/”topic”) which are essentially lists of forums on particular topics. Volunteer moderators...moderators were on top of any comments that were at all trollish. Kudos to the moderators for keeping the quality...

  18. The Challenges of Cell Culture

    Type
    Blog Post
    ...Line Authentication Committee includes a database listing all cross contaminated or misidentified cell lines...cell line provenance and identity. The Nature Publishing Group recently reiterated its strict stance on...immortal life of Henrietta Lacks. New York: Crown Publishers, 2010. 2. Gold, Michael. "A conspiracy of cells...

  19. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest...mycoplasma detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect ...possible transduction. Be consistent Once your lab establishes the best conditions for specific applications...

  20. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...tables below listing some of the most common bacterial and mammalian promoters. These lists is by no means...on its orientation to your gene Although this list is a great place to start, the tables above do not...
Showing: 321 - 340 of 771 results