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1. Plasmids 101: Multicistronic Vectors

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   ...specific 2A peptide will ultimately depend on a number of factors such as cell type or experimental conditions...

2. Antibodies 101: Producing Recombinant Antibodies

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   ...bands or a few strong ones, consider increasing the number of washes performed during your harvest. Validation...

3. Getting to Know Expansion Microscopy

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   ...molecular biology lab, and EM is limited in the number of specific targets you can label in a sample. ...

4. Uncovering Ancestral Bias in CRISPR Research

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   ... and the lines’ genomes, and a reduction in the number of the dependencies detected. Given this new information...

5. AAVs in Retinal Gene Therapy

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   ...successes of retinal gene therapy, an ever-growing number of clinical trials for devastating genetic diseases...

6. Plasmids 101: Secondary Nanobody Toolbox

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   .../or a 3x cysteine varieties. This refers to the number of cysteine sites the nanobody has available for...

7. Simplify Cloning with in vivo Assembly

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   ...but not newly synthesized sequences, limiting the number of false positive colonies. Transformation -...

8. MXS Chaining

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   ...step, the final construct can contain an unlimited number of modules. Constraints on the order of the modules...

9. Negative Can Be Positive: Open AAV Data with Addgene

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   ...various brain regions and cell types. Power in numbers A Pubmed search for “AAV” and “brain” over the ...

10. Summer SciComm Series: Modes of Communication

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    ...microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience. ...

11. CRISPR Antimicrobials

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    ...vectors are replication-deficient. Thus, a very large number of phagemids would be needed for an in vivo therapy...

12. Cpf1: A New Tool for CRISPR Genome Editing

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    ...homology-directed repair (HDR). Cpf1 also expands the number of sites that can be targeted by CRISPR to AT-rich...

13. A Career in Grants: How To Become a Grant Professional

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    ...development targeted to the grants profession? The number of organizations, both public and private, that...

14. Your Lentiviral Plasmid FAQs Answered

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    ...SIN. Packaging plasmid(s) contain the minimal number of HIV genes required for virus production (3 or...

15. Hot Plasmids Spring 2024

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    ....  PE7 shows enhanced editing efficiencies in a number of cell types at different genomic loci — in some...

16. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...on for your best options. There are an expanding number of Cas9s with looser PAM requirements compared ...

17. New CRISPR Tools: Cas7-11 and PASTE

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    ...dropped” in the wrong place, while increasing the number of potential applications. But they weren’t done...

18. Deciphering the Mysteries of Behavior with Viral Vectors

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    ...here is only a small selection of the increasing number of studies using viral vectors in behavioral neuroscience...

19. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    ...and Eric Wieschaus. "Mutations affecting segment number and polarity in Drosophila." Nature287.5785 (1980...

20. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ... bacteria in favorable conditions double their numbers rapidly. The knowledge the Singletons gained from...