We narrowed to 651 results for: PPL
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TypeBlog Post...wanted to continue doing research, Doench went on to apply to graduate school at MIT. Doench excitedly remembers...Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute: “The model that...
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Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal brightness... -
Clear and Accessible Writing
TypeBlog Post...principles we used in our daily content writing applied to alt text. We also were aware of the need for...start small with alt text or immediately dive into applying these four steps elsewhere, engaging in this practice... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...the key components of EvolvR and its potential applications. Challenges of existing mutagenesis methods...more than one mutation near the target site. Applications of EvolvR The Dueber Lab used EvolvR to discover... -
Antibodies 101: ChIP
TypeBlog Post...next-gen sequencing. ChIP is an incredibly useful application for exploring these interactions, and can give... upfront investment than many other antibody applications, this investment can well be worth it once you... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...) How many loci can Cas9 modify? To test the application of this system to gRNA multiplexing, Xie et al...multiplexing further illustrates the potential of PTGs. Applications of PTGs The major advantage of the PTG system... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...associated with molecular plant parts, particularly for applied projects intended for commercialization. Many of...patented, and building our own novel pieces where applicable. We will also be working with open source, transformation... -
Antibodies 101: Conjugation
TypeBlog Post...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo to a host... S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5),... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...help answer? Ramanathan et al. outline three applications of RaPID: Discovery. To discover novel RNA-... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...It’s at Fred Hutch.” And I hadn’t seen it. So I applied and I got it.” Science writing at Fred Hutchinson...internship similar to Susan’s NEI internship. Application information can be found here Find tips for using... -
Visualizing Protein Turnover In Situ
TypeBlog Post...proteins with FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid... observed with fluorescence microscopy. This application of AHA labeling is known as fluorescence noncanonical... -
Summer SciComm: Preprints
TypeBlog Post...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, social...listing “Submitted” articles in their CV or grant applications. A number of organizations (such as ASAPbio)... -
Antibodies 101: Secondary Antibodies
TypeBlog Post...IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies are useful..., the flexibility of their use varies across applications. Western blots, for instance, almost always ... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers...