Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 653 results for: PPL

Showing: 321 - 340 of 653 results

  1. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the...

  2. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...

  3. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

  4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...) How many loci can Cas9 modify? To test the application of this system to gRNA multiplexing, Xie et al...multiplexing further illustrates the potential of PTGs. Applications of PTGs The major advantage of the PTG system...

  5. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...associated with molecular plant parts, particularly for applied projects intended for commercialization. Many of...patented, and building our own novel pieces where applicable. We will also be working with open source, transformation...

  6. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...types or purposes of each given container, when applicable Any other relevant information Prescribing an...potentially ambiguous terms. Defining words and applying annotation can be particularly useful in academic...

  7. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...help answer? Ramanathan et al. outline three applications of RaPID: Discovery. To discover novel RNA-...

  9. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...proteins with FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid... observed with fluorescence microscopy. This application of AHA labeling is known as fluorescence noncanonical...

  12. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...gRNA binding a non-mammalian sequence (GAL4) was supplied. In addition to labeling repetitive telomeric...single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. Figure 2: CRISPRainbow...

  13. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main...

  14. Summer SciComm: Preprints

    Type
    Blog Post
    ...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, social...listing “Submitted” articles in their CV or grant applications. A number of organizations (such as ASAPbio)...

  15. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies are useful..., the flexibility of their use varies across applications. Western blots, for instance, almost always ...

  17. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo to a host... S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5),...

  18. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...end up finding something surprisingly useful. To apply these principles in a biological setting, we first...reactions, using only nature’s building blocks. Applications are quickly expanding with these kinds of Click...

  19. Antibodies 101: Conjugation

    Type
    Blog Post
    ...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...

  20. RNA Extraction Without A Kit

    Type
    Blog Post
    ...plasticware is readily available from scientific suppliers and glassware should be treated with a DEPC solution...potentially complicate results of a downstream application such as quantitative PCR to assess gene expression...
Showing: 321 - 340 of 653 results