We narrowed to 749 results for: REV;

...Site-Specific Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating ...P. Bentley The Weissman and Gilbert Labs have previously deposited their CRISPRoff system — a programmable...the cells you are studying. Antibodies can help reveal cellular identities by targeting marker proteins...

... details. You DO NOT need to present a detailed review of your field or the finer points of your techniques...avoid the temptation to provide too many details. Reverse engineer your talk. Identify the key points you...This means that once you get an interview, your previous success will not necessarily translate into getting...

...gathering data. Former lab members can give open, revealing information about the culture and lab head. While...intimidation, sexual harassment Isolation – including preventing access to opportunities, physical or social isolation...record notes and observations so you have data to review when making a decision. If you don’t see a culture...

...cells compared to monomers, although this can be prevented via antibiotic selection (Crozat, 2014). How to... multimerization, and in some cases completely prevent the formation of plasmid multimers (Bedbrook & ...overgrowing the bacterial culture Another way to prevent plasmid multimerization is to avoid growing the...

...water bath with distilled water, not tap water, to prevent salts from accumulating on the surfaces of the ...evaporates from the tub. Disinfectant may be added to prevent growth of bacteria or fungi. There are disinfectants...water bath cover on the top of the water bath to prevent evaporation and maintain the desired temperature...in the lab, you can leave the water in it from previous uses. However, you will still need to regularly...

...biased and unbiased approaches. As suggested in a review by Tycko et al., 2016, unbiased sequencing and ...High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature...specificities of CRISPR-Cas9 nucleases." Nature Reviews Genetics17.5 (2016): 300-312. PubMed PMID: 27087594...

...-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. ...

...ITR: ITR Forward Primer: 5’-CGGCCTCAGTGAGCGA ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT ITR Probe: -FAM-CACTCCCTCTCTGCGCGCTCG-BBQ...Forward ITR Primer (10 µM) 1.8 µL 16.2 µL 900 nM Reverse ITR Primer (10 µM) 1.8 µL 16.2 µL 900 nM Nuclease-free...tube, gently uncap the tubes. Add 5 µL of the previously aliquoted 1X dilution buffer from step 9 to the...titering using ddPCR, read our blog post. Last reviewed on: August 31, 2023...

... affects the solubility of the RNA. Pro-Tip To prevent overdrying, watch the pellet and carefully remove...for RNA. Store your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw ... for RNA.Store your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw ...

...inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change the U...of inosine from DNA is likely infrequent, thus preventing the induction of base excision repair. In terms...improved base editing capacity, even at sites previously difficult to target. ABE8s can achieve 98-99%...

...Biosensor Plasmids Blog: Luminescence Content last reviewed: 26 September 2025 Do you have suggestions for...

...20807748 (Link opens in a new window) Content last reviewed: 12 August 2025...

...that enables high school students to publish peer-reviewed scientific research. You’ll meet some of the folks...mouth and personal networking!) and the manuscript review/submission process at JEI. Chapter 3 - JEI target...

...DeckTheLab and tagging us, @addgene to be judged! In previous years, scientists have decorated their labs with...on the entries, select up to three winners, and reveal the winning entries on Twitter and Instagram! Happy...

...microbiology lab every year for over 20 years. Previously, the lab used three different species for the...continues until all quadrants are used, hopefully revealing single, isolated colonies in the last quadrant...

...might need to weigh it out in the fume hood to prevent exposure. Read the reagent’s material safety data...used to contain the material you’re weighing and prevent cross-contamination with other substances. They...spatula is clean, and sterile (if necessary), to prevent any potential contamination. Scoop out the material...

... expression of specific genes in the brain in reversible fashion. And that’s exactly what the LITEs now...response can be graded with light dose and is fully reversible after light retraction. Finally, because light...

...identify mutations of unknown importance in cancer Previously available methods for investigating and functionally...that that confer proliferation advantages will be revealed in the absence of growth factor. The team assigned...

...honest feedback DePace’s yearly planning meetings revolve around two simple worksheets, each of which is ...sections, the trainee speaks first for each section, preventing the meeting from feeling like an assessment or...

... stage is often much easier said than done. As reviewed in our Plasmids 101 series, proteins are overexpressed...protein expression and purification: a comprehensive review of affinity tags and microbial applications." Biotechnology...