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  1. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... is fast and can cut in as little as 2 hours.  Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org...Expanded serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes...

  4. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...it’s best to try those that have already been published first. Because behavioral optogenetics is performed...

  5. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...reels in the non-target strand of DNA much like a fisherman would reel in a big catch. It was originally suspected... Alyssa Shepard...

  6. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted how chromatin... Lalanne, J.-B., Nathans, J. F., Lee, D. S., & Shendure, J. (2024). Chromatin context-dependent regulation...

  8. Of Myc and Men

    Type
    Blog Post
    ...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring...

  9. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...

  10. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... genome that's then packaged into AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses... only the cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging ...

  12. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...temperature for mammalian cells and one which diminishes the endonuclease activity of the enzyme. In vivo...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received...

  14. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits...

  16. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...structure and could also cause your target to be washed away. Furthermore, these chemicals can also alter...: Always always always look at your antibody datasheet to see if it has been tested in IF and if a protocol...

  19. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...Small’ data produced by the research group in spreadsheets, Word docs, and the like is likely stored in... documents, but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or ...

  20. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...combined all the domains into a single protein, shedding the complexity of its ancestors. They classified...CRISPR 101: RNA Editing With Cas13  CRISPR Cheat Sheet Resources at Addgene.org CRISPR Guide...
Showing: 321 - 340 of 594 results