We narrowed to 605 results for: SHE

...expanded organoids. This work, and many others, pushed the field forward to where it is today. Below we...because of its potential for allogenic “off-the-shelf” treatment. In short, instead of using the patient...

...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus...

...of growing collections of music. I still have a shelf full of CDs that have been gathering dust since ...tested for antigen binding. If starting from an established hybridoma line, the antibody can be converted...

...involves preparation of the tissue sample, several washes, and temperature changes to visualize the expression..., is available from Addgene. If you use these published protocols and want to share pictures, feedback...

...landscape facing all scientists, both young and established, as they embark on or continue their careers .... and Social Media Content Specialist at Thermo Fisher Scientific Developing effective science advocates...

... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...it’s best to try those that have already been published first. Because behavioral optogenetics is performed...

... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...

...reels in the non-target strand of DNA much like a fisherman would reel in a big catch. It was originally suspected... Alyssa Shepard...

...Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted how chromatin... Lalanne, J.-B., Nathans, J. F., Lee, D. S., & Shendure, J. (2024). Chromatin context-dependent regulation...

...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring...

... that cannot be distinguished using conventional flow can (sometimes) be distinguished using spectral ...

... genome that's then packaged into AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses... only the cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging ...

...well as premixed and ready-to-use reagents. Thermo Fisher Scientific Inc., Origene, and NEB® are all good...you. Happy cloning (and be sure to deposit your finished constructs at Addgene!). References 1. Li, Chaokun...

...temperature for mammalian cells and one which diminishes the endonuclease activity of the enzyme. In vivo...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received...

...dissemination of your improved protocol. Many scientists, publishers, and small start-ups are turning to software ...– I didn’t get a smart phone until after I had finished my PhD and left the lab for non-bench work – but...

...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits...

...section. The GFP story has its own Franklin in Doug Prasher, who originally isolated the GFP gene but was not...produced by the quieter, more reserved Franklins and Prashers will find it increasingly difficult to compete...

...structure and could also cause your target to be washed away. Furthermore, these chemicals can also alter...: Always always always look at your antibody datasheet to see if it has been tested in IF and if a protocol...

...in the lab are more disheartening than seeing a potentially exciting result quashed by a bad standard curve...

...classic, net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...In past decades, further refinements to this established dye structure have yielded commercial panels ...