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  1. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...conjugated in the lab. In the indirect approach, you can simply keep a stock of conjugated secondary antibodies...regardless of the number of protein targets, simplifying multiplex assays.  A brief note Since the indirect...

  3. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...protein fusions, and multiple cloning sites. In the simplest case we have a standard backbone with one insert...reactions in parallel. We have a few tricks to simplify the process. First, nearly all of our Sanger sequencing...

  4. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...

  5. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...cutting most anywhere within the RNA will do. Similarly, if you want to knock out a gene, a frameshift...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...

  6. CRISPR in the Clinic

    Type
    Blog Post
    ...therapy currently in clinical trials is somewhat similar (Hussein et al). It employs Cas9 to cut at two ...therapy, since TTR is primarily made in the liver. Similarly, for cardiovascular disease approaches targeting...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...plasmid deposit process can be broken down into four simple steps: Send plasmid information to Addgene Material...the Deposit Spreadsheet is the quickest option. Simply download the spreadsheet and copy and paste your...

  9. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene...classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate into the host...

  10. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice... from each other. In a direct approach, that is simple - but what about when you need to use an indirect...

  11. Overcoming the Challenges of Lentiviral Production

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    Blog Post
    ... For some transgenes, transfection optimization simply is not enough to get a sufficient titer; this is...volumes of lentivirus routinely, you may opt for simply increasing the number of 10 cm2 dishes. While this...

  15. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...their parents. Here’s how to cross mice, using a simple example with the goal is to generate mice that ...raised in the same environment and will harbor similar epigenetic modifications. Special breeding cases...

  18. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...task, action, and results. While this method seems simple, I found it hard to remember to describe all four...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...
Showing: 321 - 340 of 649 results