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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 616 results for: TIL

Showing: 321 - 340 of 616 results

  1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...cells in the population, so some of the cells will still appear green despite having active Cas9 (see tip...site in the middle of the PCR product, you would still be able to identify the cleavage product. 3. The...

  2. Fluorescence Titering Assay

    Type
    Protocol
    ...percentage of fluorescent cells, these cells can still be used in downstream applications. Safety Warnings...

  3. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...Sheet Microscopy Light Sheet Microscopy typically utilizes a configuration of two or more objectives to create...monolayers of live cells with fluorescently labelled, motile structures These samples are thin enough to sit...

  5. Typing CRISPR Systems

    Type
    Blog Post
    ...we call CRISPR, by any other name would…probably still edit genomes. Understanding the different types... in archaea, though Class 1 remains largely underutilized compared to Class 2. Class 1 Despite their incredible...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... cancer, multiple immunotherapy targets can be utilized at the same time. A combination of tolerance marker...the development of immunotherapeutics. There are still many important questions in this exciting field ...

  8. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...armed with the highest-competency cells you will still struggle to get transformants if the plasmid you...buffers used to ensure proper lysis, but you will still face column capacity limitations since the columns...

  9. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...CGBEs. What's next? Base transversion editing is still advancing rapidly. If it follows a similar path ... synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post about...

  10. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...control but will not make the plasmids available until: you notify us with publication details or permission...that will make it easier for scientists to best utilize your plasmids (these can be protocols, GenBank ...

  13. Colony Formation Titering Assay

    Type
    Protocol
    ...antibiotic-containing media should not be added until 48–72 h after transduction. Gently aspirate the ...

  14. Deep Dive: qPCR

    Type
    Blog Post
    ...sample when developing the protocol and optimized until the multiplexed and individual values match across... isn’t the end of your analysis process. You’ll still need to do statistical analysis to determine if ...

  17. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...). Efficient retrograde neuronal transduction utilizing self-complementary AAV1. Mol Ther 16, 296–301....Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation. Neuron 84(2), 292–309. PubMed ...

  18. Technical Design of a Western Blot

    Type
    Blog Post
    ... contain any denaturing agents. However, you’ll still have to lyse your proteins. For this post, we are...systems are fast (often <10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They...

  19. Pouring LB Agar Plates

    Type
    Protocol
    ...condensation. The plates are then stored at 4 ℃ until use. Once your plates have solidified and dried,...
Showing: 321 - 340 of 616 results