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  2. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...control but will not make the plasmids available until you provide us with updated publication information...that will make it easier for scientists to best utilize your plasmids (these can be protocols, GenBank ...

  3. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...CGBEs. What's next? Base transversion editing is still advancing rapidly. If it follows a similar path ... synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post about...

  4. Fluorescence Titering Assay

    Type
    Protocol
    ...percentage of fluorescent cells, these cells can still be used in downstream applications. Safety Warnings...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...sample when developing the protocol and optimized until the multiplexed and individual values match across... isn’t the end of your analysis process. You’ll still need to do statistical analysis to determine if ...

  8. Colony Formation Titering Assay

    Type
    Protocol
    ...antibiotic-containing media should not be added until 48–72 h after transduction. Gently aspirate the ...

  9. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...). Efficient retrograde neuronal transduction utilizing self-complementary AAV1. Mol Ther 16, 296–301....Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation. Neuron 84(2), 292–309. PubMed ...

  10. Technical Design of a Western Blot

    Type
    Blog Post
    ... contain any denaturing agents. However, you’ll still have to lyse your proteins. For this post, we are...systems are fast (often <10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They...

  13. Pouring LB Agar Plates

    Type
    Protocol
    ...condensation. The plates are then stored at 4 ℃ until use. Once your plates have solidified and dried,...

  15. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...These are only starting recommendations; you will still need to experimentally determine the best titer ...efficiency across species points to its potential utility in translational applications and in models such...

  16. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ... be posting these animations regularly from now until May, so follow us to get updates whenever a new ...

  18. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...regalo permitirá que más personas accedan a recursos útiles para sus experimentos CRISPR. Muchas gracias a...
Showing: 321 - 340 of 607 results