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...Immunosorbent Assay) Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...activated patterning of dye-labeled molecules on surfaces.” Journal of the American Chemical Society 125.27...

...displayed on the surface of a cell of interest, where it can label interacting cell surface proteins with...capable of promiscuously labeling various cell surface proteins that contain a monoglycine residue at ...

...target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target cleavage. This test can...assay. This reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ...

...reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription of... for an “escape” virus to propagate and infect adjacent cells. It will be important to determine if CRISPR...application can benefit many others and accelerate the pace of research. We at Addgene hope to see the problem...

... characterised antibodies against all human extracellular proteins with community-based validation. They...experiments on similar subjects in a single, searchable space. Finally there are new publishing outlets like F1000...their experimental designs. There are many more facets to reproducibility issues in the biological sciences...

...needed to replicate inside a host cell but with space to insert your genetic cargo of choice. After hearing...GAG), and are (typically) tethered to the cell surface by a GPI-anchor. Image courtesy of IPI.   ...(LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The authors characterized ...

... Modification. Pharmaceutics, 13(5), 750. https://doi.org/10.3390/pharmaceutics13050750 Murlidharan, G...GABA neuron, for instance, may not transduce the adjacent norepinephrine neuron at all. There may be more...

...you want to know if they co-localize at the same place in the cell? Or you know that the presence of several...your microscope. Abcam's sample protocol is a good place to get started.  One important consideration when...the offending red-green image, go to Image>Color>Replace Red with Magenta. Or you can use Color>Split Channels...

...PBAD), and the A118 gene downstream of the diacetylphloroglucinol (DAPG)-inducible promoter (PPhlF).  Output...we used to drive recombinase expression may be replaced by sensors that correspond to the desired inputs...are composed. Fear not – the state machine design space is highly degenerate and often times there are multiple...

...the run, it got “dirty”, with multiple sequence traces overlapping each other! When the count was done...nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with a human gene. Advantages...comes to larger scale genome editing, such as replacement of a mouse gene with its human ortholog (greater...

...restriction enzymes that allow your insert to be placed into the backbone in the proper orientation. For... restriction sites EcoRI, XhoI, and HindIII. To place your gene in the proper orientation downstream of... important to have nice crisp bands and to have space to cut out the bands. Because of this we recommend...

...of the world, professional internationalization faces several obstacles; for instance there are few resources...career are common problems that many others have faced yet, still, this challenge did not hinder them from...evaluation of the pilot program IMP 2014/2015, took place on July 27th, 2015 at a dedicated international ...

...packaging plasmid caused the Psi sequence to be placed on the packaging plasmid forming a vector that ... Alternatively, your gene and the marker can be placed under the control of two different promoters. Both... Tet-off system a Tet-response element (TRE) is placed upstream of the promoter in your transfer vector...

...that’s where you’re at too, it’s a perfectly great place to begin. Here’s my advice: Reflect on your personal... next four years. But college, and science, are places for learning and growth! As you learn more about....org/10.1126/science.aba8039 Cordova FA (2016) Embrace uncertainty. Science 351:994–994 . https://doi....

...teamwork was what drew me to the school in the first place. The famous discoveries and experiments I’d been...at Addgene, an unofficial title that I happily embrace (and admittedly, I may have started that nickname...sometimes even necessary, to build a team in the first place? Let’s think about some of the advantages of team-based...

...Overview of using Lambda Red recombineering system to replace a gene of interest with an antibiotic resistance...as non-selectable DNA fragments, such as gene replacements and tags.  The typical frequency of recombinants....e. make changes to the third base pair of the adjacent 4-5 codons that alter the nucleotide sequence ...

...government, evidence to inform policies, a protected place to work, funding for science, and numerous other...every political persuasion) must be humble in the face of evidence. Stephanie Hays is moving from engineering...Harvard University to studying an evolutionary arms race at University of California, Berkeley. She is a ...

...ultrasound (FUS), intracorporeal (IC), intracerebroventricular (ICV), intramuscular (IM), intrathecal ...many of the same restrictions, like limited cargo space (~4.7 kb) for your genetic payload — there are a...enhancers, and switches all take up precious cargo space in AAV vectors. Dual expression systems, which use..., 2019). LY6A is functionally expressed on the surface of brain endothelial cells in C57BL/6J mice, but...

... year to give our lab and shipping teams enough space at HQ and for some of us, that means spending more...co-workers” have made their way onto our Slack workspace over the last year. Now, we’ve collected photos...

...Are you following us on Twitter or Instagram (or Facebook or LinkedIn) but want to hear and see even more...behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be...