We narrowed to 345 results for: ada
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TypeBlog Post...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...
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Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...C3 show magnified detail of the box in C. Image adapted from Andrews et al., 2019 under CC-BY 4.0 license... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post.... Jonkers, M. van Lohuizen, A. Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for ... -
Important Considerations When Using AAVs
TypeBlog Post...Skeletal Muscle AAV1, AAV6, AAV7, AAV8, AAV9 Adapted from https://www.addgene.org/viral-vectors/aav/... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...deaminase (evolved from a tRNA deaminase enzyme, TadA) to induce a A→G mutation at the target genomic ... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...closer look at three different transposon systems adapted for use as research tools: Sleeping beauty, PiggyBac... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...Lavis Lab (Grimm et al., 2017). These dyes were adapted into ligands for HaloTag® and are currently offered... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...fluorophores in relation to the laser type. Table adapted from: https://emea.bd.com/panel-design/en/flow-... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...be at any one of many stages of synthesis and degradation. For this reason it is virtually impossible to... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post... radiation therapy (owing to contributions from Madame Curie), chemotherapy, and surgical removal of the... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...J. M., Xu, A., Sisley, T. A., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Efficient C•G-to-G•C ... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...with a uracil DNA glycosylase inhibitor with a degradation tag (LVA tag) to achieve specific point mutations... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post... entered using our online form or by using a downloadable spreadsheet. Either choice can be used for deposits... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post... a table listing some of critical differences (adapted from Zhou and Melton, 2008, [43]): Characteristic... -
Antibody Validation for Flow Cytometry
TypeBlog Post...monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., 2024. Once an antibody... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...CASANOVA system is versatile in that it can be adapted to regulate gene editing, turn on transcription... -
Delivery Methods for Generating iPSCs
TypeBlog Post...42. PubMed PMID: 21339765. Methods figures were adapted from this reference. 1. Malik, Nasir, and Mahendra... -
CRISPR Guide
TypeGuide...Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine base editors, the evolved TadA domain is fused...on a second ADAR variant that displays improved specificity, and editors carrying an ADAR truncation that...break, Cas3 initiates single-strand DNA degradation. This degradation can continue for many kilobases in one...Publications CRISPR Overview Bacteria have an interesting adaptive immune response that detects and eliminates foreign...target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most popular genome...inhibiting Cas9’s endonuclease domain, or inducing degradation of the Cas9 protein. Most Acr proteins are specific... or dCas9 to create the adenine base editor. The TadA domain has also been further evolved to catalyze... -
Chemogenetics Guide
TypeGuide... 25587888 (Link opens in a new window) Coward P, Wada HG, Falk MS, Chan SD, Meng F, et al. (1998). Controlling...opens in a new window) Farrell MS, Pei Y, Wan Y, Yadav PN, Daigle TL, Urban DJ, Lee H-M, Sciaky N, Simmons...Shimojo M, Zhang M-R, Tomita Y, Nakahara J, Suhara T, Takada M, Higuchi M, Jin J, Roth BL, Minamimoto T (2020... -
Plan Your Experiment
TypeGuide...different Cas proteins available, and researchers can adapt these experiments for different organisms. This .... D., Dionne, D., Burks, T., Raychowdhury, R., Adamson, B., Norman, T. M., Lander, E. S., Weissman, J....PMID: 35941224 Gilbert, L. A., Horlbeck, M. A., Adamson, B., Villalta, J. E., Chen, Y., Whitehead, E. H...