We narrowed to 344 results for: ada

1. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   ...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...

2. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   .... Jonkers, M. van Lohuizen, A. Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for ...

3. Plasmids 101: Using Transposons in the Lab

   Type

   Blog Post

   ...closer look at three different transposon systems adapted for use as research tools: Sleeping beauty, PiggyBac...

4. A History of Genome Engineering in Popular Culture

   Type

   Blog Post

   ...deaminase (evolved from a tRNA deaminase enzyme, TadA) to induce a A→G mutation at the target genomic ...

5. Important Considerations When Using AAVs

   Type

   Blog Post

   ...Skeletal Muscle AAV1, AAV6, AAV7, AAV8, AAV9 Adapted from https://www.addgene.org/viral-vectors/aav/...

6. Antibodies 101: Designing Your First Flow Panel

   Type

   Blog Post

   ...fluorophores in relation to the laser type. Table adapted from: https://emea.bd.com/panel-design/en/flow-...

7. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

   Type

   Blog Post

   ...be at any one of many stages of synthesis and degradation. For this reason it is virtually impossible to...

8. Cancer and the Immune System: Deciphering the Relationship

   Type

   Blog Post

   ... radiation therapy (owing to contributions from Madame Curie), chemotherapy, and surgical removal of the...

9. How to Deposit Your Plasmids with Addgene

   Type

   Blog Post

   ... entered using our online form or by using a downloadable spreadsheet. Either choice can be used for deposits...

10. CRISPR 101: Cytosine Transversion Editors

    Type

    Blog Post

    ...J. M., Xu, A., Sisley, T. A., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Efficient C•G-to-G•C ...

11. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    ...with a uracil DNA glycosylase inhibitor with a degradation tag (LVA tag) to achieve specific point mutations...

12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    ... a table listing some of critical differences (adapted from Zhou and Melton, 2008, [43]): Characteristic...

13. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    ...monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., 2024.   Once an antibody...

14. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    ...CASANOVA system is versatile in that it can be adapted to regulate gene editing, turn on transcription...

15. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    ...42. PubMed PMID: 21339765. Methods figures were adapted from this reference. 1. Malik, Nasir, and Mahendra...

16. CRISPR Guide

    Type

    Guide

    ...Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine base editors, the evolved TadA domain is fused...on a second ADAR variant that displays improved specificity, and editors carrying an ADAR truncation that...break, Cas3 initiates single-strand DNA degradation. This degradation can continue for many kilobases in one...Publications CRISPR Overview Bacteria have an interesting adaptive immune response that detects and eliminates foreign...target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most popular genome...inhibiting Cas9’s endonuclease domain; or inducing degradation of the Cas9 protein. Most Acr proteins are specific... or dCas9 to create the adenine base editor. The TadA domain has also been further evolved to catalyze...

17. Chemogenetics Guide

    Type

    Guide

    ... 25587888 (Link opens in a new window) Coward P, Wada HG, Falk MS, Chan SD, Meng F, et al. (1998). Controlling...opens in a new window) Farrell MS, Pei Y, Wan Y, Yadav PN, Daigle TL, Urban DJ, Lee H-M, Sciaky N, Simmons...Shimojo M, Zhang M-R, Tomita Y, Nakahara J, Suhara T, Takada M, Higuchi M, Jin J, Roth BL, Minamimoto T (2020...

18. Plan Your Experiment

    Type

    Guide

    ...different Cas proteins available, and researchers can adapt these experiments for different organisms. This .... D., Dionne, D., Burks, T., Raychowdhury, R., Adamson, B., Norman, T. M., Lander, E. S., Weissman, J....PMID: 35941224 Gilbert, L. A., Horlbeck, M. A., Adamson, B., Villalta, J. E., Chen, Y., Whitehead, E. H...

19. Adenovirus Guide

    Type

    Guide

    ...immunogenic because they activate both innate and adaptive immune responses in the host. More than 80% of...structural capsid proteins. Glossary Term Definition Adaptive immunity Specific and long-lasting immune response...

20. Science Guides

    Type

    Guide

    ...alindromic R epeat (CRISPR) systems, which form an adaptive immune system in bacteria, have been modified ...