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We narrowed to 716 results for: cas

Showing: 321 - 340 of 716 results

  1. CRISPR Challenges: Standardization and Homology Directed Repair

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    Blog Post
    ...RNA with Cas13a (C2c2) Maybe you’d like to avoid editing the genome and just stick to RNA? Cas13 may be ...Corn’s lab describe how a better understanding of Cas9 biophysics can help enhance HDR. CRISPR 101: Ribonucleoprotein...The PAM Requirement and Expanding CRISPR Beyond SpCas9 The closer your edit is to the PAM site, the higher...repair. Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing...tool to help you design, pick, and close guides for Cas9 and Cpf1. This tool supports over 250 genomes, and...

  2. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ... all influenced by an understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS”...Medicine described Casilio, a system that uses modular RNA binding proteins, dCas9, and a modified gRNA... the Casilio system works and how you can customize it for your experimental needs. Note: Casilio is no...

  3. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...Alyssa Cecchetelli  Listen to the C. elegans RNAi podcast segment RNA interference (RNAi) is extensively ...Eric Perkins  Listen to the TurboID and miniTurbo podcast segment A fundamental approach to studying membranes...by Izzy Mueller  Listen to the viral detection podcast segment As the primary cause for both the stomach... the absence of the target RNA sequence (in this case, norovirus RNA), the ribosome binding site is inaccessible...gene on the toehold switch to be expressed. In this case, the gene encodes the LacZ𝛼 protein. Once expressed...contributed by Michael Lemieux  Listen to the SH3 domain podcast segment The human proteome contains a breadth of...

  4. Design Tips for Prime Editing

    Type
    Blog Post
    ...editing technique that relies on a fusion enzyme of Cas9 nickase + reverse transcriptase and a prime editing...., 2019), disrupting its canonical structure and Cas9 binding (Nishimasu et al., 2014). Figure...pegRNAs that use other bases in this location bind to Cas9 with canonical sgRNA structure (right). Created ...along with your intended edit. This prevents the Cas9 nickase from re-binding and nicking the newly synthesized...Zhang, F., & Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, ...doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....

  5. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post
    ...from the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold ...and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression of a catalytically...catalytically inactive (dead), human codon-optimized Cas9 under the control of Murine Stem Cell retrovirus...carries CRE recombinase and a puromycin resistance cassette. No time to produce virus? Get the ready-to-use... (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together with expression of ...

  6. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ... the ability to correctly assemble a lacI/lacZ cassette (designed by NEB for use in optimization of assembly...sequence for beta-galactosidase in the lacI/lacZ cassette. Additional confirmation of accurate assemblies...colonies showed a mixture of mis-assemblies and very occasional uncut or cut/re-ligated pGGA destination plasmids...12- and 24-fragment assemblies of the lacI/lacZ cassette, and illustrates how the volume of the 1 ml outgrowth...and assembly efficiency Five fragment lacI/lacZ cassette assembly was easily achievable with high levels... 12- and 24-fragment versions of the lacI/lacZ cassette were designed and synthesized. In conjunction ...

  7. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added ...Currently, ICE is configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency...topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility RNA Editing with Cas13 and REPAIR ...

  8. Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

    Type
    Blog Post
    ...In today’s podcast, we sit down with Wei Leong Chew, a researcher at the Genome Institute of Singapore... Listen to the interview with Wei Leong Chew! Podcast breakdown Intro: Wei Leong Chew's career path and...the audio engineer and producer for the Addgene Podcast.      Additional Resources from the Addgene Blog...featured topic page Check out our Hot Plasmids Podcast Listen to our interview with synthetic biology ... addgene podcast...

  9. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...Sapozhnikov tells us how to use dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation...-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh and Jonathan Gootenberg... worked with us to share their new CRISPR tools, Cas7-11, a low-toxicity RNA editing tool, and PASTE, ...ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger Daniel...

  10. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ... hampered by the fact that even small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially...based system, the efficiency of gene editing is occasionally less than 1%, and virtually never exceeds 40%...et al 2017 use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in ...: PMC3277219. 6. Ohmori, Tsukasa, et al. "CRISPR/Cas9-mediated genome editing via postnatal administration...Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports 20.3 (2017...

  11. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    ...interview and happy listening! Download the podcast here! Podcast breakdown (Time : Topic) 0:00 - 1:25 : Introduction...In the third installment in our podcast series, we chat with new Addgene Board Member, Michael Koeris...the audio engineer and producer for the Addgene Podcast.     Additional Resources Visit the Sample 6 website...the Addgene Blog Check the Other Posts in Our Podcast Series Get Additional Startup Advice Learn How ...

  12. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...? While properly designed perturbations – in the case of screens using CRISPR technology, gRNAs to knockout...formats: all-in-one (lentiCRISPRv2, expressing both Cas9 and an individual gRNA from this single vector) ...only an gRNA, for use with cells that already have Cas9). Each library consists of approximately 3,000 gRNAs...activity and minimize off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180...Learn to Conduct Genome-wide Screens with CRISPR/Cas9 Get John Doench's Tips on gRNA Design Check Out ...

  13. Antibodies 101: Fc Effector Functions

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    Blog Post
    ...binding affinity (Janda et al., 2016; Torres & Casadevall, 2008) but…does it really do anything? Fc functions...an antigen-bound antibody and induce (or, in the case of the Fc receptors FcγRIIB1 and FcγRIIB, inhibit...system's classical complement pathway, starting a cascade of events that results in the pores being opened....2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects on Variable...articles/10.3389/fmicb.2016.00022 Torres, M., & Casadevall, A. (2008). The immunoglobulin constant region...

  14. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...inactivates or completely removes (in the case of Gateway cloning) one cassette. Counterselection allows the scientist... Scientists introduce an antibiotic resistance cassette within the coding region of the gene they are ...is best practice not to use the same resistance cassette for the mutation and for plasmid selection. Additionally... vector typically has two separate resistance cassettes and your gene of interest is cloned into/inactivates...

  15. Antibodies 101: Chimeric Antibodies

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    Blog Post
    ... the flexibility needed for multiplexing. In the case above, you can’t costain with your desired antibodies...easily manipulated into more useful tools. In the case of isotype conversion, the variable regions of a... species and/or isotype (Liddell 2013). In most cases, the resulting chimeric antibody retains the binding...properties (Morelock 1994). While in the majority of cases the differences are subtle, chimeric antibodies ...when multiplexing with chimeric antibodies. In the case above you should use an anti-rat IgG1 secondary ...

  16. Intro to Our Summer SciComm Series!

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    Blog Post
    ...independence, such as museums, YouTube channels and podcasts, science pub nights, and pop sci books. Most scicomm... that is widely used but has a very specific use-case (definition) in a specific field.  For example, ...also students in AP biology classes Creating a podcast about genetics using dog breeds as an example ...interested in breeding dogs who also enjoy listening to podcasts A series of Tweets breaking down a new publication...others from accessing their work. But that’s not the case. In fact, it’s really the opposite. Unlike emails...

  17. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ... carefully presented case for better more diverse science. It's a series of case studies in what happens... other works of Dr. Sacks, Musicophilia includes case studies of relevant neurological atypicalities, ...the Dark Lord rules all, the books center around a cast of endearing characters who decide to take matters...Ice Age to the 1980s, Sarum follows an intriguing cast of characters from five different families over ...

  18. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ... PhD Podcast episode 15 "Simple tricks for time management: the pomodoro technique": This podcast talks... References and Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out...out about Zotero here Dear Grad Student podcast episode 2 "Essential Grad School Resources: Citation Managers...Managers, Programming, & Productivity": This podcast talks about Mendeley, Zotero, and other citation ...

  19. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post
    ... to help you find your desired plasmid. In many cases, you will need a screening strategy as well. In ...comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab..., without the need to prep your plasmid. In this case, you’d be using colony PCR to detect your insert...the other primer anneals to the backbone. In this case, if there is no insert in the plasmid, you won’t...order of nucleotides within a DNA molecule, in this case a plasmid. Sequencing is one of the surest ways ...

  20. dTAG - You're it!

    Type
    Blog Post
    ...selective degradation of FKBP12F36V-BRD4 using CRISPR/Cas9-mediated knock-in, and to evaluate in vivo degradation... and endogenous ENL was inactivated using CRISPR/Cas9 gene-editing. Featured molecules: dTAG-7 and dTAG...and endogenous MELK was inactivated using CRISPR/Cas9 gene-editing. Featured molecules: dTAG-7, dTAG-13...engineered to express YY1-FKBP12F36V using CRISPR/Cas9-mediated knock-in. Featured molecule: dTAG-47 Additional...Knock-In and Conditional Mouse Models CRISPR 101: Cas9 Nickase Design and Homology Directed Repair Plasmids...
Showing: 321 - 340 of 716 results