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We narrowed to 452 results for: crispr

Showing: 321 - 340 of 452 results
  1. Plasmids 101: Using Transposons in the Lab

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    Blog Post
    ...encodes a Type I-F CRISPR-Cas system. This system has four major components: 1) a CRISPR RNA (crRNA), 2) ...sets the stage for combining the super powers of CRISPR and transposons to allow for the targeted delivery...
  2. Important Considerations When Using AAVs

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    Blog Post
    ...system. Currently, AAVs are being used to deliver CRISPR/Cas9 but have the potential to deliver other therapeutic...be used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient...Gene Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional ...
  3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post
    ... expression, plant expression, mouse targeting, CRISPR, TALEN, synthetic biology, affinity reagent/antibody...Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment Suggest A Plasmid You'd Like to Find...
  4. Plasmids 101: Degron Tags

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    Blog Post
    ...several advantages over the alternative systems – CRISPR, siRNA, etc. Firstly, they are reversible mechanisms...10.1073/pnas.1216880109  Resources on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging...
  5. Your Lentiviral Plasmid FAQs Answered

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    Blog Post
    ...shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not surprisingly, we ...Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr Library...
  6. Plasmids for Stem Cell Research

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    ...Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms ...Nat Commun. 2016 Mar 7;7:10869. Hu CRISPRa Human CRISPR activator system for reprogramming human cells ...enogenous genes Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski... Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN Human Transcription Factor-Mediated Differentiation...
  7. Hot Plasmids: Summer 2025

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    ... TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search for smaller...more in our recent blog post on TnpB). Similar to CRISPR-Cas, TnpB is directed to genomic targets using ...
  8. AAVs in Retinal Gene Therapy

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    Blog Post
    ...trials for prevalent diseases - to name a few. CRISPR-based tools are also particularly well suited to.... Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina. Front Cell ...
  9. Addgene’s Blog Gets a New Look!

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    Blog Post
    ...showcases the five main topics of the blog: Plasmids CRISPR Viral Vectors Fluorescent Proteins Science Careers...
  10. Antibody Plasmid Collection

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    ...BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin...diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5...
  11. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ... may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the different...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology...
Showing: 321 - 340 of 452 results