We narrowed to 709 results for: esc
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TypeBlog Post...of my review - "The manuscript by ZZZ et al., describes ... The authors share a wealth of data on ... ...Additionally, you can ask that data and resources described in the manuscript are accessible to the community...
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Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...Direct reprogramming describes the process where differentiated cells are turned into a cell type of ...reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics... -
Plasmids 101: Origin of Replication
TypeBlog Post...p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (derivative) and F1** A Relaxed...multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12;52(10):1828-37... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...Antibody Guide, which include information about immunofluorescence, conjugates, isotypes, and more. How You ...Basics of Western Blotting Introduction to Immunofluorescence Addgene’s Antibody Guide The project is ... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...will not produce the fluorescent protein while areas that are exposed will fluoresce. With these images,... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...as their kidneys fail. The condition was first described more than 60 years ago, but there is still no ...changes in temperature. “The disease was first described in 1971,” McSweeney said. “But, it is still sometimes... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive opsin from ...then, there has been a steady stream of papers describing new photosynthetic or photosensory molecules ... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...and more details on how to play the game on the FRESCI blog. As I wrote in the blog post, BUILD YOUR PLASMID...Barcelona. He is also founder and consultant at FRESCI particularly interested in advanced in vitro models... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast” variant provides... studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...producer cells and purification protocols often describe a process that begins with cell lysis to release...Central PMCID: PMC4104587. György, Bence, et al. "Rescue of hearing by gene delivery to inner-ear hair cells... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction...other labs at UCSF (the QCRG network coming to the rescue -- again!). Also, we’ve got to mention that our... -
Components of CRISPR/Cas9
TypeBlog Post... of the major CRISPR lingo and will be able to describe the functions of the various CRISPR/Cas9 components...Bind to a guide RNA The guide RNA, which will be described in more detail below, enables Cas9 to cut a specific... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post...fantastic K12 education initiatives like BiteScis (https://bitescis.org/) and amazing graduate student writing... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post... articles reporting chromatin-related research describe the production of plasmids that might be useful...colleagues and guest bloggers have written posts describing new methods and tools for studying chromatin ... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...differ by only a few nucleotides, such as the fluorescent proteins EGFP and mEGFP. Many of these features...the gene, the name of its gene product, and a description of its function. You can also click one location... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...plasmid encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic by Maya... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post... Lab’s recent Nature Biotechnology paper, they describe TurboID and miniTurbo, which utilize non-toxic... money, and viruses at low concentrations often escape detection. The Alexander Green lab found a solution... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...lentiviral packaging methods. The three components described above (envelope, packaging, and transfer) are ...nomenclature of the plasmid backbone is meant to be descriptive of the components found within the backbone. ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post... NEB's Resource describing Dam and Dcm Methylases Discovery of DpnI Review describing dam-/dcm- E. coli...