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  1. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...spacer of each loxP site and the two strands are ligated, resulting in the reciprocal crossover event. This...sequence. Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites...

  2. Working with Nuclear Receptors

    Type
    Blog Post
    ...acquisition, storage, and utilization - and on self-propagation by reproduction. Both physiological processes...controlled by deliberate and compulsory actions instigated by the central nervous system signaling to peripheral...

  4. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...These results are normally one figure and can be negative and/or even replicated studies to show reproducible...naturally “associate, self-excise, and covalently ligate their flanking peptides together (Wang et al., ...

  5. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...some scientists argue that these issues can be mitigated by using low doses and running appropriate controls...pore domain (IPD) of a channel, the PSAM acts as a gating mechanism to control ion flux. The system allows...

  6. Hot Plasmids February 2024

    Type
    Blog Post
    ...ribonucleoprotein (RNP) was limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better ...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...

  10. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...of the run, each droplet is read as positive or negative for each reaction, math happens (this time, Poisson...accurately, and raw data should be checked to confirm negative samples and, if unexpected, verified by running...

  11. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...So you’ve done the research, gathered up your data into an exciting story, and are ready to present your... information to prepare for the next meeting. Navigating talks Before the conference Is the conference...

  12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...lead to improper protein localization, protein aggregation, or disruption of normal protein function, and... functionally unique repair templates. A final ligation reaction correctly assembles the final targeting...

  13. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...then need to be purified by density gradient centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

  14. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...software confirms the serotype of the prep by interrogating the VGS data for small predetermined seed sequences...could lead to transgene expression in recombinase-negative animals, producing misleading results.  To date...

  16. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...transformed into a bacterial cell. Transformed cells propagate, are induced to produce your protein of interest...of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein expression...

  17. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...elaborate if your audience seems captivated. Expertly navigate questions Answering questions is arguably the ..., so let us know your own ways to successfully navigate poster sessions with ease. Many thanks to our...

  18. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...made me happy. Grad school can provide plenty of negativity, so I volunteered at a place that made me happy...recharged. The fun change of pace helped break up the negativity and allowed me to focus on the positive. I went...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify due to NHEJ...microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID: 26701308...

  20. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...may require strains such as ccdB survival (for Gateway plasmids).  We also like to store plasmids that...
Showing: 321 - 340 of 625 results