We narrowed to 633 results for: gats

...and selectable markers that allow students to propagate the plasmids in either E. coli (ori and ampR) ...and instructor resources is available at our Investigations in Molecular Cell Biology Website. Many thanks...

...ball-and-chain that they resemble, actually impede your investigation into the regulation of your favorite gene. But...disease: cancer (Feinberg & Vogelstein 1983). The floodgates, as they say, had opened.  In 2021,  with over...

...ribonucleoprotein (RNP) was limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better ...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...

...some scientists argue that these issues can be mitigated by using low doses and running appropriate controls...pore domain (IPD) of a channel, the PSAM acts as a gating mechanism to control ion flux. The system allows...

...elaborate if your audience seems captivated. Expertly navigate questions Answering questions is arguably the ..., so let us know your own ways to successfully navigate poster sessions with ease. Many thanks to our...

...So you’ve done the research, gathered up your data into an exciting story, and are ready to present your... information to prepare for the next meeting. Navigating talks Before the conference Is the conference...

...made me happy. Grad school can provide plenty of negativity, so I volunteered at a place that made me happy...recharged. The fun change of pace helped break up the negativity and allowed me to focus on the positive. I went...

...the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify due to NHEJ...microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID: 26701308...

...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...may require strains such as ccdB survival (for Gateway plasmids).  We also like to store plasmids that...

...transformed into a bacterial cell. Transformed cells propagate, are induced to produce your protein of interest...of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein expression...

...lead to improper protein localization, protein aggregation, or disruption of normal protein function, and... functionally unique repair templates. A final ligation reaction correctly assembles the final targeting...

...software confirms the serotype of the prep by interrogating the VGS data for small predetermined seed sequences...could lead to transgene expression in recombinase-negative animals, producing misleading results.  To date...

...direct molecular interaction without further investigation. One fluorescent microscopy method commonly ... to fluorescently label a wide variety of gram negative bacteria (West Virginia University School of Medicine...

...of the run, each droplet is read as positive or negative for each reaction, math happens (this time, Poisson...accurately, and raw data should be checked to confirm negative samples and, if unexpected, verified by running...

...then need to be purified by density gradient centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways...genome-wide screening. Avoid false positives and false negatives – As with any experiment, the use of appropriate...

... placing orders per day across regions. Our investigation of model fit revealed some potential instabilities...University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution...

...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...

...Virologists too have benefited from the ability to propagate viruses in cell culture; if there were no cells...line consisting of records of the origin and propagation of the line, and can include assays for authentication...

...Also, overexpression of FPs can trigger protein aggregation which is toxic for the cells and affects the ...Furthermore, many of these FPs are cytotoxic, prone to aggregation and cross-react with anti-GFP antibodies, making...