Plasmids 101: TOPO Cloning
Type
Blog Post
... introduce your 3’-end “A” overhang insert, the magic of TOPO cloning happens.
Basic procedure
Let’s break...
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Plasmid Cloning by PCR
TypeBlog Post...that will bind to and replicate it. The following image shows the ends of the ORF and how these are used... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post... Bioblasts? Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic... -
Another Pathway into Cells: iTOP
TypeBlog Post...transduced with iTOP resulting in GFP expression. Image courtesy of Diego D'Astolfo. CRISPR/Cas9: a ... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog Post...can still creatively collaborate and brainstorm! Image from Joseph Mucira. Implement new tools for... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog Post...fragments migrate more quickly than larger fragments. Image from OpenWetWare. This method is often used preceding... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...this interactive activity during their session. Image from Reproducibility for Everyone. While this... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See our full biosensor AAV... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...the sensor for serotonin by over four orders of magnitude. Unger validated the serotonin sensor with proof-of-principle... -
K. phaffii: Rising to the Occasion
TypeBlog Post...DOI: 10.3892/br.2017.941 Bernauer L., et al., Komagataella phaffi as Emerging Model Organisms in Fundamental... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post... The Pomodoro timer is literally a tomato! Image from Gerlos. Tools for checking grammar and... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...guy has been the best: Thanks, Todd! As you can imagine, it’s also been quite the administrative headache... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...this function is not yet fully understood, we can imagine that this transport is probably not 100% efficient... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...laser scanning microscope will allow Tsakanova to image cortical neurons and study changes in neural activity... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...flashback to time spent playing a card game called “Magic”. There were complicated rules that governed how... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...Attaching the fluorescent protein fusions allowed us to image R bodies inside cells, and these constructs may ... -
Components of CRISPR/Cas9
TypeBlog Post... DNA. Thus, the end result of Cas9-mediated DNA damage is a DSB within the target DNA ~3-4 nucleotides... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...develop a bacterial camera that prints a chemical image, as well as a genetic method for the computational... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...BamHI) and run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...Figure 1: Management can seem a mystery at first! Image used under CC. Understanding the transition...