...is being involved in the scientific community important to you and your lab? Fujii: I first knew Addgene...interact with each other, which is critically important, especially for young researchers. It was a really...technologies. Majority of them are eager to just "import" technologies developed abroad, so it's a little...

...reaction creates an expression clone with all of the components necessary for gene expression.     The LR...coding sequence. If you choose this strategy, it’s important to include the proper protein expression elements...Clone When making the expression clone, it is important to choose the destination vector that best fits...

...that asbestos exposure remains the single-most important factor in developing mesothelioma. Asbestos, and...cells that can begin an immune response. This important biomarker can be used to distinguish between three...States and many other countries, and it is still imported, exported, and used throughout the world. New ...

...valuable lessons along the way. We think it’s so important that we have written plenty of articles on building...bench mentor-mentee relationship is extremely important – without a good set up it could be very difficult...help you get more out of the in-person training component. Documenting during training It’s hard to recall...

.... More importantly, the expression of this construct is Cre-dependent, enabling spatiotemporal control...are needed to deliver all the protein and RNA components, and several sgRNAs must be screened to identify...

...option for the experiment above, but it’s also important not to load yourself up with too many tasks. If...ll discuss in the planning section below, it’s important to dedicate specific amounts of time to specific...quite difficult. In my experience, planning has 3 components: A mechanism for blocking off time for different...

..., documents need to minimize jargon. Equally importantly, you really are selling yourself. A sales pitch...anyone could relate to, that people would agree is important to understand. (1-2 sentences). For example: Distinguishing... lot of time to discuss and the questions are important. However, it's much easier to focus on the questions...

... proteins in living cells and organisms. Both components of the chimera are encoded by DNA. Since researchers...2016; Lambert, 2019). These characteristics are important, but have less priority than stickiness. In my...lead to over-expression artifacts. This is an important downside of the technology and should always be...

... produce important cellular metabolites. Biotin acts as a carboxyl carrier from one compound to the other...Molecular Biology of Biotin Attachment to Proteins; Symposium: Nutrition, Biochemistry and Molecular Biology...

...24 known mammalian integrins, all heterodimers composed of one of 18 alpha and one of eight beta subunits... rAbs. The other eight are chimeric antibodies composed of rat or mouse variable regions fused with rabbit...belief that αVβ3 would be the most functionally important integrin on these cells. What’s next IPI is working...

...good article at NatureJobs: Interviews: The All-Important Job Talk. 1. Watch other people give great talks... time to prepare (and it does take time). For important talks, ask your peer mentors to check your slides...to practice and over time you can master this important science skill. Additional Resources Joanne'...

...sample per lane, onto the top of a resolving gel composed of the crosslinked polymer acrylamide. In addition...proteins are immobilized onto a membrane, usually composed of a nitrocellulose or polyvinylidene difluoride...while less protein leads to a thinner band. It is important to note, however, that you must include a loading...

...months. With CRISPR (B), you can inject CRISPR components directly into a zygote and obtain a knockout ...complexes of Cas9 protein and the separated guide RNA components (crRNA and tracrRNA) for maximum Easi-CRISPR ... simple method for delivery of genome editing components to the germline. In contrast to standard microinjection...

... Bujard, both the promoter and transactivator components of the system have been engineered and improved...revolutionized genetic studies in flies. An additional component, the GAL80 repressor, adds a repressible element... transcription from the UAS promoter. This is important because it allows them to be used together for...

...engineer cellular processes that are influenced by temporally ordered biochemical events. How do RSMs work?...gene regulation programs. The software has two components - a database of pre-compiled register designs...sites, promoters, genes, terminators, etc) are composed. Fear not – the state machine design space is ...

... protective environment where individuals are empowered to productively navigate through both their failures...how to write cover letters, and has suggested important things to emphasize in order to be selected in...pursue them, increased their network of contacts important for their career goals, and identified possible...

...homology to the target site for recombination. Key components of the Lambda Red recombineering system The lambda... lambda red recombineering system has three components (Figure 1): 1) Exo, 2) Beta, and 3) Gam. All three...an ssDNA oligo substrate.   Figure 1: Components of the Lambda Red Recombineering system. See ...

... the University of Cambridge. One of the most important steps in the CRISPR experimental process is validating...silico prediction. These techniques differ in important ways, but can also complement one another by providing...tissue from whole animal models, no exogenous component required (e.g. dsODN), doesn't require high read...

...editing technology led to the development of many important medical and agricultural products. But it also...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...SciFi is one of the most imaginative and most important genres of literary fiction. It has always served...

... phenomenon? Exploration can be important, but it is equally important to have a plan to get to mechanism...outcome, e.g. where could you publish this and how important is the result? Is there a tractable question ...