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We narrowed to 760 results for: mpo

Showing: 321 - 340 of 760 results

  1. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study...3293995. Additional Resources on the Addgene Blog Important considerations when using AAVs Learn more about...research. Visit Our AAV Guide Page for info on AAV components, common uses of AAV, and much more! Find ready-to-use...

  2. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...is being involved in the scientific community important to you and your lab? Fujii: I first knew Addgene...interact with each other, which is critically important, especially for young researchers. It was a really...technologies. Majority of them are eager to just "import" technologies developed abroad, so it's a little...

  3. How to Be an Excellent Trainee

    Type
    Blog Post
    ...valuable lessons along the way. We think it’s so important that we have written plenty of articles on building...bench mentor-mentee relationship is extremely important – without a good set up it could be very difficult...help you get more out of the in-person training component. Documenting during training It’s hard to recall...

  4. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...reaction creates an expression clone with all of the components necessary for gene expression.     The LR...coding sequence. If you choose this strategy, it’s important to include the proper protein expression elements...Clone When making the expression clone, it is important to choose the destination vector that best fits...

  5. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...that asbestos exposure remains the single-most important factor in developing mesothelioma. Asbestos, and...cells that can begin an immune response. This important biomarker can be used to distinguish between three...States and many other countries, and it is still imported, exported, and used throughout the world. New ...

  6. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    .... More importantly, the expression of this construct is Cre-dependent, enabling spatiotemporal control...are needed to deliver all the protein and RNA components, and several sgRNAs must be screened to identify...

  7. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...24 known mammalian integrins, all heterodimers composed of one of 18 alpha and one of eight beta subunits... rAbs. The other eight are chimeric antibodies composed of rat or mouse variable regions fused with rabbit...belief that αVβ3 would be the most functionally important integrin on these cells. What’s next IPI is working...

  8. Tips for Getting a Faculty Position

    Type
    Blog Post
    ..., documents need to minimize jargon. Equally importantly, you really are selling yourself. A sales pitch...anyone could relate to, that people would agree is important to understand. (1-2 sentences). For example: Distinguishing... lot of time to discuss and the questions are important. However, it's much easier to focus on the questions...

  9. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...option for the experiment above, but it’s also important not to load yourself up with too many tasks. If...ll discuss in the planning section below, it’s important to dedicate specific amounts of time to specific...quite difficult. In my experience, planning has 3 components: A mechanism for blocking off time for different...

  10. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ... proteins in living cells and organisms. Both components of the chimera are encoded by DNA. Since researchers...2016; Lambert, 2019). These characteristics are important, but have less priority than stickiness. In my...lead to over-expression artifacts. This is an important downside of the technology and should always be...

  11. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... produce important cellular metabolites. Biotin acts as a carboxyl carrier from one compound to the other...Molecular Biology of Biotin Attachment to Proteins; Symposium: Nutrition, Biochemistry and Molecular Biology...

  12. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...editing efficiency. The V106W mutation was an important addition. Improved activity of adenine base editors...secondary screen, the team tested Cas9 mutations that imposed a preference for an NGC PAM. Although all the tested...approval, as well. If the editor and delivery components could be rigorously safety tested and reused,...

  13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... out of fashion but is still useful and most importantly, effective. In the research world, there is a... can simply block translation machinery. The composition of the RISC varies, but at the very minimum requires...plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence Choosing ...

  14. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...good article at NatureJobs: Interviews: The All-Important Job Talk. 1. Watch other people give great talks... time to prepare (and it does take time). For important talks, ask your peer mentors to check your slides...to practice and over time you can master this important science skill. Additional Resources Joanne'...

  15. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... Bujard, both the promoter and transactivator components of the system have been engineered and improved...revolutionized genetic studies in flies. An additional component, the GAL80 repressor, adds a repressible element... transcription from the UAS promoter. This is important because it allows them to be used together for...

  16. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...months. With CRISPR (B), you can inject CRISPR components directly into a zygote and obtain a knockout ...complexes of Cas9 protein and the separated guide RNA components (crRNA and tracrRNA) for maximum Easi-CRISPR ... simple method for delivery of genome editing components to the germline. In contrast to standard microinjection...

  17. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...sample per lane, onto the top of a resolving gel composed of the crosslinked polymer acrylamide. In addition...proteins are immobilized onto a membrane, usually composed of a nitrocellulose or polyvinylidene difluoride...while less protein leads to a thinner band. It is important to note, however, that you must include a loading...

  18. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...engineer cellular processes that are influenced by temporally ordered biochemical events. How do RSMs work?...gene regulation programs. The software has two components - a database of pre-compiled register designs...sites, promoters, genes, terminators, etc) are composed. Fear not – the state machine design space is ...

  19. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ... protective environment where individuals are empowered to productively navigate through both their failures...how to write cover letters, and has suggested important things to emphasize in order to be selected in...pursue them, increased their network of contacts important for their career goals, and identified possible...

  20. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...homology to the target site for recombination. Key components of the Lambda Red recombineering system The lambda... lambda red recombineering system has three components (Figure 1): 1) Exo, 2) Beta, and 3) Gam. All three...an ssDNA oligo substrate.   Figure 1: Components of the Lambda Red Recombineering system. See ...
Showing: 321 - 340 of 760 results