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TypeBlog Post...their flexible and jiggly nature suggested the dynamic nature of a protein. The Bender’s popularity was...
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Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...example of multipotent cells. Unipotency: As the name suggests, cells under this category can differentiate... -
10 Basic tips for mammalian cell culture
TypeBlog Post...practice to label your plates and/or flasks with your name, date, the cell line you’re using and its passage... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...session. Exchange business cards or jot down their name and affiliation from the poster heading to get in... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...pioneered by Voie and colleagues and originally named orthogonal-plane fluorescence optical sectioning... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...manipulated to express more than two genes. Plasmid Name Multicistronic Element Expression Type MSCV-IRES-EGFP... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...result should match, enter the common or scientific name into the Organism box. This small piece of information... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post...column or an ultrafiltration concentrator. As the name suggests, you may also concentrate your prep to ... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...have their own unusual sort of virulence as well — namely that they can cause cancer via insertional mutagenesis... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...or not? — FRET is preferable when the temporal dynamics of PPIs are the focus of study. Many thanks ... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...and identified that a S375A mutation in RESCUE (named RESCUE-S) offered ~76% on-target C-to-U editing ... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...repository has played in these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...diluting the virus. It is important to note that the dynamic range of the ddPCR is between 1 and 100,000 genome... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...? The available filtering options now change dynamically based on the type of material and specific search... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...Nanobody Plasmids Structural comparison As the name suggests, nanobodies are smaller antibodies.They... -
Simplify Cloning with in vivo Assembly
TypeBlog Post... your primers and template. Amplifying the ‘unamplifiable’ Check your amplification by gel electrophoresis... -
MXS Chaining
TypeBlog Post...can generate constructs to visualize cell cycle dynamics, titrate inducible transgene expression, or a ... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...teachers, who work hard maintaining a current, dynamic curriculum, would be hard-pressed to maintain the... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...into bacterial genomes. This new capability was named INTEGRATE (Insertion of Transposable Elements by... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post...Cross-Linkers Chemical cross-linkers work by (as the name suggests) cross-linking cellular proteins. Cross-linking...