We narrowed to 388 results for: nans

1. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   ...particular type will interact. A protein with a nanomolar Kd will exist primarily as a dimer in cells while...

2. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   ...which has been difficult to modify due to NHEJ’s dominance over HDR in this species. As CRISPR technology...

3. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   ...effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer...

4. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   ...is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential...

5. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...Medical Center.  You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative...

6. Droplet Digital PCR for AAV Quantitation

   Type

   Blog Post

   ...tool for titration and integrity evaluation of recombinant adeno-associated viral vectors." Human Gene Therapy...

7. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   ...In this example we’re going to be looking for recombinant monoclonal antibody plasmids from the James Trimmer...

8. Plasmids 101: Colony PCR

   Type

   Blog Post

   ...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   ... among the earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study ...

10. How to Write a Scientific Review Article

    Type

    Blog Post

    ...deacetylases in B-and T-cell development, physiology and malignancy." Genes & cancer 6.5-6 (2015): 184. PubMed PMID...

11. Using Video to Share Your Science: We Share Science

    Type

    Blog Post

    ...continue to rely on printed journal articles as the dominant tool for sharing ideas. This leads to everyday...

12. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    ...neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV) has been widely ...

13. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    ...13. https://www.frontiersin.org/articles/10.3389/fnana.2019.00093 McDade, J. R., Waxmonsky, N. C., Swanson...

14. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    ...Leonard, A. C. (2018). Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome...

15. Hot Plasmids - November 2023

    Type

    Blog Post

    .... (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

16. Viral Production at Addgene

    Type

    Blog Post

    ...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...

17. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    ...on commercial lateral flow strips, similar to pregnancy tests. With lateral flow strips, the presence ...

18. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    ..., Kat Milligan-Myhre, and Pragati Chengappa.  Financial and moral supporters Denise Akob, Sara Alexander...

19. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    ...three-month generation time and labor-intensive maintenance compared to invertebrate model systems, and their...

20. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    ...antibodies in plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-...