We narrowed to 976 results for: nin

...off expression of one gene, while simultaneously turning on the expression of another! What Are FLEx switches...FLEx switch that turns BFP expression off, while turning on mCherry expression. For this FLEx switch to ...the first recombination step will invert the intervening DNA fragment using either loxP or lox511 sites...FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts in the inverse... genOway, we are able to distribute materials containing FLEx technology. Read the genOway press release... Michelle Cronin...

...found that many researchers feel they lack formal training in a variety of transferable skills. At Addgene... to be done (Who is responsible for making the running buffer this week?). Scientists negotiate all the... on this transferable skill without any formal training or coursework. First, you have to develop an awareness...settings, creative negotiation skills are key to maintaining and growing relationships with your colleagues... them to ensure we create a win-win situation? Learning about and respecting cultural differences is a... academic committees as a tenured professor or signing contracts when working for a business development...

...multitude of scientific techniques such as molecular cloning or molecular diagnostics.       Steps of PCR...End point PCR is most commonly used in molecular cloning, sequencing, and genotyping. It’s extremely useful...of DNA. This amplicon can be used in molecular cloning to create a plasmid of interest, for detecting ...PCR Amplify a region of interest  Molecular Cloning Site-directed mutagenesis Molecular Diagnostics...Droplet Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101: Colony PCR Site Directed ... primer  Molecular Biology Reference Molecular Cloning Techniques ...

... can be produced in many different cell types, spanning both prokaryotic and eukaryotic organisms. Typically... requires three plasmids — a transfer plasmid containing your gene of interest plus two packaging plasmids... available, so you’ll only have to worry about cloning your transfer plasmid. The transfer plasmids have...media every 12 to 24 hours. Processing the virus-containing supernatant will depend on the virus type. AAV... be produced in insect cells, but they require cloning specific AAV or lentiviral machinery into a baculovirus...bacmids are readily available, so the amount of cloning required is about the same between insect and mammalian...

...References Geertsma ER (2013) FX Cloning: A Versatile High-Throughput Cloning System for Characterization ...SB_convex. These scaffolds contain serines and threonines at positions that can be randomized for creating...protein (sybody), ribosome, and mRNA. During the screening process, displayed sybodies that bind to the biotinylated...pDX_init plasmid, using the fragment exchange (FX) cloning technique which uses Type IIS restriction sites... phage display screens are subcloned (using FX cloning) into the expression vector pSb-Init, which attaches...

...for genome-wide CRISPRi library screening experiments where maintaining library diversity is important,...-CRISPRi into bacteria. Top: A Tn7 transposon containing the CRISPRi components and a plasmid with the...integrated copy of CRISPRi. Bottom: An ICE element containing the CRISPRi components is transferred to recipient...essential genes were less represented while bacteria containing guides targeting non-essential genes remained...Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system." Nature methods 2.6 (2005...

...sequence into the Subject Sequence box that appears. Aligning two nucleotide sequences is probably the fastest...reactions are imperfect and sequence near the beginning or end of a reaction is often unreliable, we suggest...such as when you attempt to verify the starting methionine of a gene but the blastx results start the alignment... not filter low complexity regions by default, meaning that you do not have to remember to adjust any ...nucleotide or protein sequences. We also use COBALT for aligning multiple protein sequences, particularly for comparing... to help other labs speed up their plasmid and cloning verification steps and free up more time for using...

...relatively low, meaning that most Cas9-induced DSBs are repaired by non-homologous end joining. As a result...double nicking increases indel formation slightly. Designing the sgRNA with a spacer that only binds the edited...mismatch repair contributed to a large number of unintended prime editing outcomes, including indels. Therefore...in unpredictable ways. In some cases, however, combining an evolved RT domain from PE6a-d with an evolved...binding, but pegRNAs have an extended 3′ tail containing the reverse transcriptase template and primer...efficiencies compared to PEmax with normal pegRNAs, but combining PE7 with epegRNAs resulted in similar or reduced...

...Solving Creativity Data analysis Listening Negotiation Career Planning Resources Cross team communication...until it is identified by another person. Similar reasoning may also apply to other acquired skills.” It is... we are, but there is also value in knowing and owning your transferrable skills yourself so that you ...strengths and weaknesses, and view everything as a learning experience. If your first reaction to an opportunity...didn't even know you possessed. Think about your shining moments. Recall a few times you really felt great...

...tirelessly to get Addgene from a concept to an up and running repository. As they built the foundation for what..., our founders and early employees spent this remaining time by elaborately pranking each other, dressing...any have some fun together! Laura’s Top 10 Event Planning Tips! 1. Take suggestions Some of Addgene’s best...low participation numbers for an event you are planning, dangle the carrot (or more popularly- cookies...people know exactly where to be and when. This pre-planning will avoid chaos and delays in getting the fun... sure people know that there is something fun happening! Addgene actually has chimes outside of our break...

...up with the pace of deposits while maintaining quality. Maintaining high-quality plasmids for open science...flow cell membrane and have an electrical current running through them. DNA or RNA is pulled through the ...plasmids that are highly repetitive, such as those containing cassettes of the same gene multiple times.  Bioinformatics...science Addgene is committed to maintaining high QC standards for all deposited materials. The QC team ...materials through the QC process quickly while maintaining accuracy.  This post was written by Alyssa Shepard...

...genes in mouse muscle and eye tissue. Neisseria meningitidis Cas9 (NmeCas9) at 1,082 amino acids, can also...catalytic domain of RNA deaminase ADAR2. Constructs containing the ADAR2 truncation ADAR2DD(delta984-1090) are...virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo. Genome Biol 19: . https://doi.... Comander J, Liu Q, Liu DR (2020) Cytosine and adenine base editing of the brain, liver, retina, heart...Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo genome editing... Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

...generally have higher levels of background staining. Antigen-containing samples often include undesirable molecules...non-specifically to assay components and increase background staining. Direct ELISAs also lack the signal amplification...a capture antibody and then add their antigen-containing sample. The target antigen binds to the capture...antigen and then they preincubate the antigen-containing sample with their conjugated primary antibody...cross-reactivity Higher level of background stainingLess sensitivityLess specificityLimited availability...

...that can be used to treat it. For those who are running out of hope with more traditional treatments, such...mesothelioma is a form of cancer that develops in the linings of certain areas of the body, including the lung...less frequently, ingested), it can get into the lining of the lungs or travel to one of the other areas... a result, mesothelioma is often misdiagnosed, meaning that by the time it has caught it has usually started...mesothelioma – that is, mesothelioma that forms in the lining of the abdomen (peritoneum) – have the option of...Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional Resources on Addgene.org ...

...through experience. However, studying the genetic underpinnings of these processes has been complicated by the...-optimized viral vectors, and high-throughput screening approaches. Here, we’ll highlight these recent...lentiviral vectors can be found here. High-throughput screening strategies Although the tools described above ...Lab, which developed a subgenome-scale CRISPRa screening approach in embryonic stem (ES) cells to determine...from undifferentiated cells at the end of the screening process. However, while the neuronal marker TUJ1...gymnastics for sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for this particular...

...Observing a bright signal for the first time after cloning an FP fusion with your favorite protein of interest... an FP within a fusion construct [1,2], i.e. positioning of the FP sequence relative to cell compartment...C-terminus [3]. You must therefore determine your cloning strategy and decide whether you will create an ... experimental needs. Equally important, before cloning you must consider whether and how well your FP ...fluorescing (see Figure 2) [5].  When using FPs containing two cysteine residues and a sequence intended... al. "Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored proteins....

...might be contextual fear conditioning (reviewed in [4]). Contextual fear conditioning is a behavioral experiment...prolonged inhibition of CA1 neurons attenuated conditioning, which suggests that other regions of the brain...technique in neuroscience it has a bit of a steep learning curve and many opportunities for mistakes. The...University.  His interests include the role of learning and memory in opioid addiction and science communication...Kheirbek, Mazen A., et al. "Differential control of learning and anxiety along the dorsoventral axis of the...

...the most important considerations to make when planning and performing an IF experiment. What is immunofluorescence...include: permeabilization, antigen retrieval, and sectioning. Blocking Block is used to reduce non-specific...experimental goals. Example steps include: counterstaining, mounting sample on slides, and confocal   ...your most pressing research questions, but also stunning images (am I the only one with IF results as artwork...    References and resources References Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to...to Performing Immunofluorescence Staining. In: Methods in Molecular Biology. Springer New York, pp 299...

...short intracellular ends and large ectodomains containing ligand-binding heads and long legs that protrude...affinity. This extended-open state is key to strengthening ligand binding, mediating cell adhesion and ...technology, are directed to integrins that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in...displayed by a subset of the RGD-binding integrins containing the αV subunit. Using these affinity reagents...education in protein science, IPI hosts events and training courses on technologies and laboratory techniques... website and stay informed on future events by signing up for the IPI newsletter. The collection of 26...

...vectors for your specific gene, the lab recommends designing homology arms with the modification of interest...synthesis company such as IDT or Genscript, and cloning them sequentially into the chosen acceptor vector...that causes Hemophilia B. Unfortunately, work combining CRISPR with AAVs for improved genome editing is...gene fusions. The current state-of-the-art in combining the AAV and CRISPR-based approaches is described... Medicine. His research currently focuses on determining the mechanisms and consequences of cohesin gene...Epstein, Benjamin E., and David V. Schaffer. "Combining engineered nucleases with adeno-associated viral...