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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 563 results for: pac

Showing: 321 - 340 of 563 results

  1. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...

  2. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...much the spread of the virus to adjacent areas impacted the experiments we wanted to perform. It required...injection titers (1,2), and cell types used for packaging viral particles (6), can influence vector performance...

  3. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...decided to take a different approach. His own most impactful learning experiences happened in the lab, and ...analyze their recently purified MDH to study the impact of phosphorylation on the enzyme’s structure, function...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...well-documented history of racial bias, which still impacts and influences current research. Understanding ... reflects European ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency...

  5. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...Cas9’s, but with the direct repeat preceding the spacer rather than following it. The Cpf1 crRNA is also...counterparts, they will also be easier to deliver in low-capacity vectors, such as adeno-associated viral (AAV) ...

  7. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...application and submitting it, to evaluating the capacity of an organization to receive and manage funds...up-to-date on the changing guidelines and regulations impacting grant management, we track grant opportunity deadlines...

  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...from quenching by aqueous solvent. This tightly-packed structure explains the importance of the entire...stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be used, when...

  10. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...colleague and give you a million dollar startup package. There are three steps to chalk talk preparation...have developed your research vision, you need to package it as a roughly 30 minute chalk talk. You will ...

  12. How to Be an Excellent Trainee

    Type
    Blog Post
    ... walking through the protocol, by going to each space in order to double-check you remember where the ...Resources Resources on the Addgene blog Trading Spaces – Visiting Scientist Edition Mentoring for Scientists...

  13. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... clearance. It is more convenient to update the spacer(20nt) on pEcgRNA plasmid as only two 24 nt oligos... proteins Cas6, Cas7, Cas8, and the crRNA. The spacers of the crRNA can be customized as well, including...

  14. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...sharing knowledge allows for life to happen without impacting the overall work. Each of our sprints is themed...

  16. Five Popular Model Organisms

    Type
    Blog Post
    ...mice to study how different mutations in leukemia impact different treatment regimens (Zuber et al., 2009... our own cells. Like human cells, yeast DNA is packaged into chromosomes and about 23% of yeast genes ...

  18. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...consuming and difficult. Finding volunteers who had space to shoot was another hurdle. As we slowly started...strict safety protocols to keep Addgenies safe. Space was at a premium, so I had to figure out a solution...

  19. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...of interest that are found in the extracellular space. The targeted epitopes can be motifs within transmembrane...Proliferation dyes can visualize proliferative capacity and speed of cells. (a) With each division half...

  20. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...application can benefit many others and accelerate the pace of research. We at Addgene hope to see the problem...PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. ...
Showing: 321 - 340 of 563 results