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Showing: 321 - 340 of 783 results

  1. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...communication skills. So, where can a graduate student or post-doc start? The good news is there are more opportunities...science on the one you already have. Read my earlier post about Scientists on Twitter here. 4)   If you enjoy...

  2. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...chemotherapeutics, and ultraviolet light. In this post, we will describe the mechanism of homology directed... than 10 bp away if possible. Check out our blog post on Improving HDR efficiency for more details on ...highest frequency of HDR edits.  Check out our blog post on Easi-CRISPR for more details. References and...single-stranded DNA templates. Jacobi SA, et al. Poster at Keystone Precision Genome Engineering. 2017....

  3. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...This post was originally written by Joel McDade and significantly updated in 2022 by Susanna Stroik.  ...verify your genome edit can be found in our blog post entitled “CRISPR 101: Validating Your Genome Edit...More resources on the Addgene blog Read all the posts in our CRISPR 101 Series Learn about the PAM Requirements...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...Professor at Georgetown University, contributed to this post. Adeno-associated viruses (AAVs) make fantastic...enhance the efficiency of genome editing. In this post we'll walk through a variety of ways you can use...many applications of AAV in some of our other blog posts and have also been able to browse some of the ready... al. "CRISPR/Cas9-mediated genome editing via postnatal administration of AAV vector cures haemophilia...

  5. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... Plasmids 101 blog posts Learn more about the origin of replication Find blog posts about plasmid cloning...maintained in one bacterial cell. In this Plasmids 101 post, we’ll cover why this happens, how it might affect...

  6. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...This post was contributed by guest blogger Derek Simon. The actual experiments you do will be determined...topic you’re interested in studying, but, in today’s post, we’ll discuss some of the important considerations...optogenetics to be fully summarized in a short blog post, but some examples I’m personally interested in ...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

  7. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...This post was contributed by Damien Wilpitz founder and lead consutant at Experiment Designs Consulting...start up budget, create a start up list.  In this post we’ll help you successfully negotiate a start up...        Additional Resources Find other career posts on the Addgene Blog! Learn more about Experimental...

  8. Science Career Options

    Type
    Blog Post
    ...career you actually want, you can feel lost. Use this post to explore the wide range of careers available to...sending out applications. Check out our other career posts for more specifics on some careers and to find advice...

  9. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique...thymine (T) to hybridize and form hydrogen bonds. This post focuses on "sticky end" TOPO (also called TOPO-TA...

  10. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...This post was contributed by guest blogger Talley Lambert, a Research Associate at Harvard Medical School...online guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining..., using a popular database management system (PostgreSQL), that aims to eventually be a comprehensive ...

  13. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...This post was contributed by Matteo Tardelli, a postdoctoral scientist at Weill Cornell Medicine. Discussing...outside of the lab meeting setting. Check out the blog post “Fostering Communication in Remote Collaborations...blogger Matteo Tardelli! Matteo Tardelli, PhD is Postdoctoral Scientist at Weill Cornell Medicine in NYC and...

  15. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...This post was contributed by Deborah Sweet, Vice President of Editorial at Cell Press. Almost everyone...research more reproducible Read all of our blog posts on scientific publishing  ...enterprise will benefit. We’ll have fewer students and postdocs sitting frustratedly in reproducibility jail and... PhD at the MRC LMB in Cambridge UK and then a postdoc at The Scripps Research Institute in La Jolla, ..., California. She joined Elsevier after her postdoctoral studies, and has held various editorial roles...

  16. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...For more details on this technology, see our blog post Overview of AAV Encoded Calcium Sensors). We are...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral ...

  17. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ... This post was contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. ...Additional resources on the Addgene blog Find blog posts on scientific sharing Get tips on writing a review... submission to the subsequent journal.  As a postdoctoral researcher studying plant development and abiotic...HapMap population in Amsterdam. Currently, she is a postdoc at KAUST, Saudi Arabia, in the group of Prof. Mark...

  18. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...This post was contributed by guest writer Pamela J. Hines PhD, Senior Editor at Science Magazine. Although...Out Our Career Resources Subscribe to Career Blog Posts Resources at Addgene.org Find Job Openings at ...

  20. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’... detailed investigations into the release of DNA post-cut. Using substrate DNA labeled on both sides of... optional donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating...
Showing: 321 - 340 of 783 results