...structure-based framework engineering. Methods, 34(2), 184–199. PubMed PMID: 15312672. Inbar, D., Hochman...variable portions of heavy and light chains. Proceedings of the National Academy of Sciences of the United...

...delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused...bells and whistles. The fibers are made from a process called thermal drawing, essentially where blocks...

...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...ways, and the advanced search page simplifies the process of building advanced multi-parametric search queries...

...created for the Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids...talk about the amplification and verification processes we use to ensure high-quality library distribution...

... remember that a resume is typically no more than 2 pages long. Write your resume for the job posting ...familiar with the publishing or patent application processes? How about facilitating a collaboration between...

...establishing the dynamic range of your FRET assay (Figure 2). These labeling methods are freely interchangeable...PMID: 20161833. PubMed Central PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding...Ca2+ measurements using improved cameleons." Proceedings of the National Academy of Sciences 96.5 (1999...

...about colony PCR in this blog post.   Figure 2: The steps of colony PCR.   Sanger sequencing Sanger...analyze plasmids, which can help you visualize this process, or visit our restriction analysis blog post. ...now use next-generation sequencing during our QC process. This way, we can confirm the sequence of the entire...

...this year. Designing the donor plasmid Figure 2: Donor plasmid design strategy. A donor plasmid contains...cell’s inherent homology directed repair (HDR) process following the double strand break CRISPR/Cas9 makes...stem cell will seek out a template for the repair process. By flooding the cell with a plasmid containing...cell imaging to study cellular structures and processes and can lead to the better understanding of cell...

... to remove this default setting. Timesaving Tip #2: blastx searches are inherently slower than blastn...results we analyze as part of our quality control process has steadily grown. On a busy week, we may need...than 200 plasmids as part of our quality control process. Consequently our team has refined our use of the...might also enjoy seeing how our quality control process has changed with the introduction of next generation... reference/database sequence is ≥ 95%. [Our QC process would be trouble-free and much faster if 95% of...

... enrich the microvesicles containing AAVs (Figure 2). Since the development of the exo-AAV isolation protocol...cells and purification protocols often describe a process that begins with cell lysis to release AAV particles...

...perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547 Kunda, Z. (...polarized beliefs on controversial science topics. Proceedings of the National Academy of Sciences of the United...

...responsible for that differentiation.   Fig. 2: Single cell RNA-seq clustering of cells transduced...combinatorial overexpression programs on cellular processes. For help with library transformation and amplification...

...retroviral library was used to transduce T helper type 2 (Th2) cells from mice that constitutively express ...interrogation of the genes required for cellular processes. For a successful screen, it is necessary to identify...

...understand the processes that happen inside the cell,” says Pedre Pérez. Figure 2: Micrographs ...from our extremities. “Here, painful stimuli are processed by a network of excitatory and inhibitory neurons...

...beginning Let’s take a quick flash back to 2004. “Shrek 2” was the most popular movie in theaters, the Razr ...meant facilitating the Material Transfer Agreement process, which was a major barrier for depositors and requestors... requestors alike. We transformed this process by aligning institutions with standardized agreements and...

...strain (which will be covered in more detail in part 2 of this blog series) controls the cell types in which... the nucleotide sequence during the DNA repair process. The addition of small molecules that promote homologous...Also, because non-homologous repair is a random process, zygotes edited using the same nuclease may not...

...aspects of the work you presented may also be unclear. 2. Make the audience your primary focus When writing...and engage with the public, and learn from the process of writing papers and proposals.  To get the most...

...to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling with subclass-switched recombinant... methodologies drew me to learn more about the process that was used to create this important collection...

...axon terminals to the soma (retrograde tracer) (Fig. 2). Viruses as neuronal scalpels Viruses can be injected...way the brain connects across these regions, a process driven by neurons. By studying how neurons are ...

...the necessary steps to perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline...output from the first process to the second process. For example, our bbduk process created trimmed FASTQ...pipeline, we wrote a separate Nextflow process. In Nextflow, a process defines which command or script will...specifying the output of an initial process as the input to a subsequent process, we linked each step in our ...pipeline steps into processes and then connect the steps using the output from one process as input to the... Nextflow process.  Next, we specified the input and output channels for the initial process and tested...was clear: to build one process at a time and connect each subsequent process to the existing ones. Once...