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Showing: 1 - 20 of 24 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...mouse somatic cells. Cell Stem Cell. 2008 Feb 7. 2(2):151-9. Jaenisch Lentivirus Mouse Polycistronic, ...with transgene-free induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83...somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. ... temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek Fibroblasts...conversion of human fibroblasts to dopaminergic neurons. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8...self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32....Nature of Induced Pluripotency. Cell. 2015 Jul 16;162(2):412-24. Mikkelsen Lentivirus Human Expression of ...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...of basal H 2 O 2 levels with peroxiredoxin-based probes Real-time monitoring of basal H 2 O 2 levels with...Belousov Hydrogen Peroxide (H 2 O 2 ) Cytosolic or mitochondrial sensor for H 2 O 2 oxidation (roGFP2-Orp1) ...peroxiredoxin-2-based probe. Nat Commun. 2018 Aug 7;9(1):3145. Hadley Sikes Hydrogen Peroxide (H 2 O 2 ) Monitoring...Calcium Red fluorescent calcium sensors (fRCaMP1/2, GECO1/2) The kinetic mechanisms of fast-decay red-fluorescent...intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten Merkx Zinc eZinCh-2 Zn2+ FRET ... eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging...(6):e38591. Sakiko Okumoto Hydrogen Peroxide (H 2 O 2 ) Fluorescent reporter of cytosolic hydrogen peroxide...

  3. Cre-lox system

    Type
    Collection
    ... Site-Specific Recombination in Transgenic Mice. Proc Natl Acad Sci. Jul 15;89(14):6232-6. PubMed . Matsuda...Transgenes Introduced by In Vivo Electroporation. Proc Natl Acad Sci. Jan 16;104(3):1027-32. doi: 10.1073...Cells by the Cre Recombinase of Bacteriophage P1. Proc Natl Acad Sci. Jul;85(14):5166-70. PubMed . Sternberg...Cre-lox-Regulated Conditional RNA Interference from Transgenes. Proc Natl Acad Sci. Jul 13;101(28):10380-5. doi:10.1073...Cre-ERT2 CAG Mammalian Heller 112622 pVHC_PGKneoLox2DTA.2 Venus, Cre-ERT2, targeting vector with MCS for homology...homology arms Mammalian Heller 112623 pEHC_PGKneoLox2DTA.2 Emerald, Cre-ERT2, targeting vector with MCS for homology... arms Mammalian Heller 112624 pmTHC_PGKneoLox2DTA.2 TFP, Cre-ERT2, targeting vector with MCS for homology...

  4. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...make the pre-CRISPR RNA (pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special ...CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A . 112(10):3002-7. PMID: 25713381...2015. Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain effector) Type I (Cas3) Type ... with unprecedented speed and specificity. Figure 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA...regulation of transcription in eukaryotes. Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino...Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell . 163(3):759-71. PMID: 26422227...arrays. Separate Cas proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs...

  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ...immunoglobulin heavy diversity 2-15 D2, IGHD215 IGHD2-2 immunoglobulin heavy diversity 2-2 IGHD22 IGHD2-21 immunoglobulin...heat shock 70kDa protein 2 HSP70-2, HSP70-3 HSPA4 heat shock 70kDa protein 4 APG-2, HS24/P52, MGC131852, ...variable 2-33 (non-functional) IGLV233, V1-9 IGLV2-8 immunoglobulin lambda variable 2-8 IGLV28, V1-2 IGLV3...beta 4 DEFB-2, DEFB102, DEFB2, HBD-2, SAP1 EDN1 endothelin 1 ET1, HDLCQ7 EDN2 endothelin 2 ET2, PPET2 ...receptor subfamily 2, group C, member 1 TR2 NR2C2 nuclear receptor subfamily 2, group C, member 2 TAK1, TR2R1...suppression of tumorigenicity 2 - STC1 stanniocalcin 1 STC STC2 stanniocalcin 2 STC-2, STCRP TAC1 tachykinin,...HPS, HPS2, PE AZGP1 alpha-2-glycoprotein 1, zinc-binding ZA2G, ZAG B2M beta-2-microglobulin - CALR calreticulin...

  6. COVID-19 Resources

    Type
    Collection
    ...page: SARS-CoV-2 Antibodies : Recombinant monoclonal antibodies that target SARS-CoV-2 nucleocapsid protein...protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing SARS-CoV-2 sequences. SARS-CoV... targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86...targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids...packaging plasmids. SARS-CoV-2 Lentiviral preps now available for several SARS-CoV-2 constructs from the Krogan...SARS-CoV-2 plasmids are curated on a separate Ginkgo Bioworks Plasmid Collection page. SARS-CoV-2 Pooled...Converting Enzyme 2) is the host cell receptor mediating the entry of SARS-CoV and SARS-CoV-2 viruses. ...

  7. Lentiviral Prep Service

    Type
    Collection
    ...141391 pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro Expression of SARS-CoV-2 nucleocapsid (N) protein Krogan...141386 pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro Expression of SARS-CoV-2 membrane (M) protein Krogan...141385 pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro Expression of SARS-CoV-2 envelope (E) protein Krogan...141383 pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro Expression of SARS-CoV-2 orf3a protein Krogan 141347...Barcode Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding...pBOB-CAG-SARS-CoV2-Spike-HA Expression of SARS-CoV-2 spike (S) protein Pao 154985 pWPI-IRES-Puro-Ak-ACE2...barcoding library. Version 2 of the Celltag library contains 4934 barcodes to combinatorially index cells for...

  8. mTOR Pathway

    Type
    Collection
    ...known as RPS6KB1; Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau...Rictor RPTOR independent companion of MTOR, complex 2 SGK Serum/glucocorticoid regulated kinase 1 Return...disease. Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93. doi: 10.1016/j.cell.2012.03.017. PubMed PMID...fuel these energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome...regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein and lipid synthesis...

  9. Antibody Guide

    Type
    Collection
    ...cells; function is unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible ...such as anaphylactic shock Monomer with a valency of 2 IgG The most abundant isotype in blood, also found...secondary immune response Monomer with a valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary... ranging from antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include...used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared to alternative...randomized reorganization process, creating a unique binding site. This process, known as V(D)J recombination...delaying a process through sequential rounds of validation. Validation of an antibody is a process separate...

  10. CRISPR Guide

    Type
    Collection
    ...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A . 112(10):3002-7. PMID: 25713381...Sequences Glossary Publications CRISPR Overview Class 2 C lustered R egularly I nterspaced S hort P alindromic...systems enable researchers to target anywhere from 2 to 7 genetic loci by cloning multiple gRNAs into a... is included on the gRNA-containing plasmid, or a 2-plasmid system in which Cas9 must be delivered separately...library (panel E ). Remember - if you are using a 2-vector system, you will need to transduce cells that...Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing...

  11. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...Addgene collection? Click here to start the deposit process and have your plasmids added to the list. You can...more information and assistance with the deposit process. Want more information on the wide variety of Cas...

  12. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...referred to as intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the...standard consisting of ECFP and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting...Förster resonance energy transfer, or FRET , is a process by which energy is non-radiatively transferred ...

  13. Distribution to Industry

    Type
    Collection
    ...Information Featured Collections COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from ...more about Addgene’s MTA process please visit our Help Center . The MTA Process: Once your order has been...approve the MTA documents, your order will be processed and shipped. Industry Distribution Policy A portion...

  14. Plasmids for Optogenetics Research

    Type
    Collection
    ...optogenetic tool is the widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ChR2 is a ...manipulate cells and their governing biomolecular processes. Plasmid...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...8661 p4455 FLAG-hPLIC-2 UBQLN2 Flag CMV ALS Peter Howley 8662 p4456 FLAG-hPLIC-2 NTF UBQLN2 Flag CMV ALS...196208 GB1-A11(2-196)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196209 GB1-A11(2-196, G38R)-Strep...196210 GB1-A11(2-196, D40G)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196211 GB1-A11(2-196, G175R...196212 GB1-A11(2-196, G189E)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196213 GB1-A11(2-52)-Strep ...TLS 1: hTLS.pBSKS(+) FUS T7 ALS David Ron 21828 TLS 2: hTLS.pCDNA1 FUS CMV ALS David Ron 21829 TLS 3: TLS.pRSET.C...ApoE Alzheimer's Sohail Tavazoie 51436 pGL3Basic-ME.2/ApoEpromoter APOE ApoE Alzheimer's Sohail Tavazoie...T7 ALS Nicolas Fawzi 127195 RP1B FUS 1-163 QQ4xSS #2 FUS His T7 ALS Nicolas Fawzi 127196 pJ411 FUS 1-163...

  16. Neurodegeneration Research Collection

    Type
    Collection
    ...different inherited genes: Presenilin 1, Presenilin 2, and APP gene.The majority (>90%) of individuals develop...with the iPSC toolbox . Lam et al. bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression...disease and implicate a wide range of cellular processes. The vast majority of cases (90-95%) of ALS are...

  17. CRISPR Plasmids - Tagging

    Type
    Collection
    ...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh...Protocols, 2016 . Yamamoto Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh -...conventional HDR-mediated gene knock-in. The detailed procedure can be found in Sakuma et al., Nature Protocols...

  18. CRISPR References and Information

    Type
    Collection
    ...Mammalian Cell Lines CasPEDIA : An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...families, HDR oligonucleotide design, and batch processing for designing genome-wide gRNA libraries. PubMed...

  19. EXtracellular Plasmid RESource (EXPRESs) Consortium

    Type
    Collection
    ...Durocher et al. Nucleic Acids Research 2002 Jan 15;30(2):E9 A list of commonly-used bait and prey plasmids...pathways that are important in cellular recognition processes. Laboratories interested in these approaches have...

  20. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...BclI-SwaI none S. pyogenes URA3 Wyrick pCRISPomyces-2 61737 Bacteria BbsI yes, cut S. pyogenes Apramycin... S. pyogenes Zhang gRNA Cloning Vector Bbs I ver. 2 85586 Mammalian BbsI None S. pyogenes H Fujii pX601...with the endogenous tRNA-processing system Plant Utilizes the tRNA processing system to reduce size and...Editing in Monocot and Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA expression ...Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise multiple functional gRNAs...
Showing: 1 - 20 of 24 results