We narrowed to 396 results for: promoter
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TypeBlog Post...localization sequence) and expressing it from a CAGGs promoter. However FLPe is still less efficient than Cre...
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Viral Vectors 101: Pseudotyping
TypeBlog Post...encoded on the envelope plasmid which contains a promoter, the envelope gene, and a polyA tail. ... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...beneficial for many systems. How the EDGE Program promotes scientific sharing The EDGE Program is designed... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...iteron-containing plasmids, RepA can also bind to its own promoter and inhibit its own transcription. As copy number... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...interest. A low primer-to-template ratio is used to promote annealing of one primer to each template. The PCR... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow... -
10 Steps to a Perfect Science Talk
TypeBlog Post...non-interview setting. There are many resources on self-promotion (how hard it is for some people, especially women... -
Site Directed Mutagenesis by PCR
TypeBlog Post... in mapping of regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp genomic fragment containing... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...enough to prevent confluency but high enough to promote cell growth. Overgrown cultures stimulate quiescence... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...reinforces behavior (activation of the circuit promotes subsequent, repeated activation), this is an approximation... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter 48961 Worm ...of two sgRNAs from independent U6 promoters and Cas9 from CBh promoter. Ventura A CRISPR/Cas9 toolkit for...along with 1-4 sgRNAs expressed from independent promoters. sgRNAs are cloned into separate vectors and then... assembly of single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one...pGGDestTol2LC vector. Uses five distinct zebrafish U6 promoters for sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs...expression of two gRNAs from Drosophila U6:1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous ... -
Serotype Testing AAV
TypeCollection...Boyden ) and direct GFP expression from the CAG promoter. For information about each catalog item, including...direct EGFP expression from the human synpasin promoter. For information about each catalog item, including... -
Plan Your Experiment
TypeCollection...template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing efficiency...prime editing. CRISPR Intereference : Target the promoter region of the gene of interest, where the Cas9...approaches, the species and expression pattern of the promoter for both your Cas enzyme and gRNA and the presence...the wide range of options for the Cas enzyme, promoters, and selection markers. There are also no packaging...to specific cell types by using cell-specific promoters, which is useful for in vivo studies. While we...transfect, transduce, or may not express common promoters. Browse Cas9 plasmids for RNP delivery . As mentioned... -
Synthetic Biology - Assembly Standards Guide
TypeCollection.... Therefore, the same reaction which ligates a promoter into an empty backbone can be repeated to ligate...ligate your gene of interest after the promoter, and so on. The following table provides information for... -
Control AAV Preps
TypeCollection...that express fluorescent proteins under general promoters. These AAV can be used to compare the activity...PHP.V1 CAP-B10 CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants...Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP... -
AAV Molecular Tools
TypeCollection...Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator...pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of the tet-off... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...When you go on interviews or are looking for a promotion, have some solid examples of times when you have... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven expression system. We use a single polycistronic... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...that binds specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but...