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...Watson-Crick base pair, are joined with nearly the same efficiency as a correct base pair. DNA ligases have... resistance-mediating polymorphisms in African samples of Plasmodium falciparum." Journal of clinical ...

...are working with proto-oncogenes or with human samples that have not been tested for HIV.  If not using...ribosome entry site (IRES) and expressed under the same promoter. Alternatively, your gene and the marker...

...general anesthesia (Taylor et al., 2016). These very same neurons also play a central role in motivated behaviors.... Hirsch, Matthew L., Sonya J. Wolf, and R. J. Samulski. "Delivering transgenic DNA exceeding the carrying...

...cytoplasmic background signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes...doi.org/10.1038/s41592-021-01338-6  Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C.,...

...validation screen using selected gRNAs extracted from the same or another library. These different approaches could...Listed you can also easily extract gRNAs for the same gene from several libraries, and identify those ...

... library is a set of plasmids all built with the same backbone and only differing in the gene/insert. ... Pro Tip! If you have multiple plasmids with the same gene or vector backbone, you can copy some information...

.... This can complicate comparisons made between samples, i.e. transgene expression and hence the extent...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...

... similar to using a single chimeric gRNA. At the same time, the off-target effects are reduced because...genomic PCR, but couldn't amplify the EMX1 gene using same primer you used in the Science paper (Cong et al...

...follow us on TikTok for bite-sized versions of the same. ...

...find out the final four! (Note: For cases where the same name was submitted by more than one person, we selected...

...to search many different antibody catalogs at the same time.  If you're looking for an antibody, especially...

...I was bored this weekend while waiting to take samples from my assay at like 23:00, so I decided to make...

... using the link or use the text below to get the same information. Download a PDF of CRISPR Cheat Sheet...

...for the presence of pathogens in environmental samples via PCR. We hope these plasmids will help Dr. Duedu...

...lines of emerging global challenges. However, these same researchers face significant barriers to scientific...

... assessment of many protein targets in a single sample; 3) producing recombinant, partially humanized ...

...to modify the expression of multiple genes at the same time.” For more details on how to apply the new ...

...gene/target, name, application, pass/fail rating, sample species, antibody species, or whether the results...

...working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite part of their...

...our organization. One thing that will remain the same is Addgene’s commitment to supporting the research...