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We narrowed to 664 results for: sam

Showing: 321 - 340 of 664 results

  1. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... as a proxy for your edit of interest. Using the same gRNA and donor, you can deliver a silent mutation...to compare the sequencing reads from your edited sample to an untreated population. Software such as CRISPResso...

  2. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Cells often express multiple integrins that bind the same ligand, making it difficult to dissect the function...extracellular matrix. During immune responses, this same conformation encourages immune cell adhesion and...

  3. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...activate or repress target genes in plants using the same general experimental design principles developed... expression of both Cas9 and the gRNA off of the same plasmid. Delivering CRISPR components to plant cells...

  4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...doi.org/10.1074/jbc.M101559200 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated virus serotypes....2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna, M., & Samulski, R. J. (2006). Α2,3 and α2,6 N-Linked Sialic Acids...

  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...system to GAL4/UAS that functions in essentially the same manner, with LexA binding to the lexA operator (...desirable to control or look at multiple genes at the same time.  The 3 binary systems listed above are orthogonal...

  9. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...are working with proto-oncogenes or with human samples that have not been tested for HIV.  If not using...ribosome entry site (IRES) and expressed under the same promoter. Alternatively, your gene and the marker...

  11. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ... about these methods is that they all follow the same general cyclical process (Figure 2). The differences...codons (different codon sequences that code for the same amino acid) for various amino acids. Essentially...

  12. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...validation screen using selected gRNAs extracted from the same or another library. These different approaches could...Listed you can also easily extract gRNAs for the same gene from several libraries, and identify those ...

  14. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ... library is a set of plasmids all built with the same backbone and only differing in the gene/insert. ... Pro Tip! If you have multiple plasmids with the same gene or vector backbone, you can copy some information...

  15. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    .... This can complicate comparisons made between samples, i.e. transgene expression and hence the extent...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...

  16. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ... similar to using a single chimeric gRNA. At the same time, the off-target effects are reduced because...genomic PCR, but couldn't amplify the EMX1 gene using same primer you used in the Science paper (Cong et al...

  20. Deck the Lab 2022!

    Type
    Blog Post
    ...I was bored this weekend while waiting to take samples from my assay at like 23:00, so I decided to make...
Showing: 321 - 340 of 664 results