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We narrowed to 538 results for: shi

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Showing: 321 - 340 of 538 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...approximately 1 in 5000 males. DMD is caused primarily by frameshift mutations in dystrophin, a protein essential ...regions, so low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) ...

  2. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...experimental condition you could look for a slight size shift in the protein band before and after treatment.  ...0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender ...

  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...of AAV1 and AAV2, with only six amino acids distinguishing its capsids from those of AAV1 (Issa et al....N. L., Puschnik, A. S., Davulcu, O., Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M....

  4. A Control for All Seasons

    Type
    Blog Post
    ... western blot and look for the molecular weight shifts indicative of tau phosphorylation. By including... standard (P) to check for tau molecular weight shifts. A negative control is a sample or group that...

  7. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...member who knows how the physical kits are made and shipped; Chiara, the tech transfer expert who understands...critical features based on my own experience: Leadership Few things are more frustrating than being on...

  8. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...Dronpa protein (named after dron, a ninja term for vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated...PMID:16550175. 6.Ando, Ryoko, Hideaki Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling...

  9. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...inside the cells and are thus typically used for distinguishing live from dead cells. The combination of antibody-fluorophores...again. In the beginning, we talked about the relationship between a photon's wavelength and energy, how...

  10. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...channels. In a typical assay a user will compare the shifting intensity of events. Since intensity positively...positively correlates with expression, the further the shift of a cell from the negative control, the higher ...

  11. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...energy transfer values you can expect, thereby establishing the dynamic range of your FRET assay (Figure...PubMed Central PMCID: PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements...

  12. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...plasmid DNA from your cleared lysate to a substrate, washing away left-over debris that carried over from the...bottles and the overall weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN...

  13. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...Principal Investigator (PI), provide us with your shipping address for deposit materials, and agree to our...prepare your plasmids and a prepaid label with shipping materials to send your plasmids to us. Essentially...

  14. Optogenetics Guide

    Type
    Guide
    ... VChR1 Red-shifted channelrhodopsin from Volvox carteri 570 Chronos High-speed, red-shifted channel from...Red-shifted channelrhodopsin from Tiarina fusus ; variants include rsChRmine (further red-shifted), hsChRmine...PsChR1 Red-shifted chloride-conducting channel from Proteomonas sulcata 540 Phobos Blue-shifted iC++ variant...Stigeoclonium helveticum 500 Chrimson, ChrimsonR Red-shifted channel from Chlamydomonas noctigama ; ChrimsonR...and frChRmine (combined high speed and more red-shifted); good response with 585 nm or 650 nm stimulation...rsChRmine, frChRmine) PsChR2 High-efficiency blue-shifted channelrhodopsin from Platymonas subcordiformis...soma-targeted variant soCoChR 470 CheRiff Blue-shifted channelrhodopsin from Scherffelia dubia 460 C1C2...

  17. CRISPR Guide Update

    Type
    Blog Post
    ...widely adopted in the past few years, as well as refreshing the existing content. Access the CRISPR Guide...

  19. We’re Going Viral This Week!

    Type
    Blog Post
    ... the sharing of reagents and data, we’re also publishing research. Addgene’s research team also investigates...
Showing: 321 - 340 of 538 results