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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 772 results for: LIS

Showing: 341 - 360 of 772 results

  1. The Challenges of Cell Culture

    Type
    Blog Post
    ...Line Authentication Committee includes a database listing all cross contaminated or misidentified cell lines...cell line provenance and identity. The Nature Publishing Group recently reiterated its strict stance on...immortal life of Henrietta Lacks. New York: Crown Publishers, 2010. 2. Gold, Michael. "A conspiracy of cells...

  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest...mycoplasma detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect ...possible transduction. Be consistent Once your lab establishes the best conditions for specific applications...

  3. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...tables below listing some of the most common bacterial and mammalian promoters. These lists is by no means...on its orientation to your gene Although this list is a great place to start, the tables above do not...

  4. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...The following is a description of a technique published by Simpson, Sacher, and Szymanski in January 2016... for cell-binding abilities (6). This can be accomplished using a restriction enzyme with a short recognition...PubMed PMID: 26275959. 2. Weigele, Peter, and Elisabeth A. Raleigh. "Biosynthesis and Function of Modified...

  5. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...were Spanish scientists (MD, PhD and MD/PhD) established in some of the top Universities in USA and Europe...laboratories to start working on projects together. List of discussion topics at IMP In these sessions, mentors... Certified neurologist and multiple sclerosis specialist, currently President and owner of THiNKBrain,...

  6. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...screening techniques such as ELISA and cell surface display are common. ELISA enables quantitative measurement...support common research tools like western blots, ELISAs, immunoprecipitation, and flow cytometry. The better...

  7. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...used existing BRAF gRNA viruses, but you can find a list of validated gRNA plasmids on our website. There...amplified a 500-600 bp product. The primers I designed (listed above) worked well and I got clear bands using ... I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi:...

  8. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at UC Berkeley. Most of ... As scientists, we probe beyond the realm of established theory. Realizing that textbooks don’t tell us...blogger! Eleanor Wang is currently a Research Specialist in Patrick Hsu’s lab at UC Berkeley. She will...

  9. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based team in academia...are currently about 5.5 million articles in the English version of Wikipedia alone. Humans have been forced...snapped out of the phase of my life that had me listening to Simon & Garfunkel’s “I Am A Rock” on repeat...

  11. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    ...names and we’ve recently chosen four names as finalists. Now, we’re asking you to help us make the decision...submitted by more than one person, we selected the finalist based on the first time of submission.)     ...

  12. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...use of pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad usefulness of pMD2...CRISPR, and so much more (find the full, searchable list here). Tips for using pMD2.G At Addgene, we use ...

  13. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...new forms of science communication. Click here to listen to the interview with Tom McFadden!   1. Rosalind... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  14. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...concrete advice on how to pick a lab and a project. Listen to the interview here! Podcast Breakdown (Time ... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  17. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...research infrastructure that has taken decades to establish.These funding decisions will have far-reaching consequences...difficult to maintain or access. These services can be listed as direct costs from a CRO in grant applications...

  18. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...blot, immunohistochemistry, immunocytochemistry, ELISA — but in all cases, it contains a detailed description...completeness. The information is then formatted and published as a report with a DOI for citation. If you’re...

  19. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ... One obvious way to do that is and has been to publish in journals with a high “impact factor,” a measure...to say all of us here would add materials to that list (plasmids, yay!). Scientists are blogging, talking...
Showing: 341 - 360 of 772 results