We narrowed to 905 results for: NSI

... hard about picking a good mentor, and begin considering career options early. Tune in for great advice...year dip (see graph). Niroshi and Ben give their insights as to what causes this dip. 11:11 - 15:37 : How...

...developed a platform called SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single...further to provide multiplexed detection, higher sensitivity, and more. Read the paper in Science Find...

...journals has risen steadily from 1995 to 2013. Considering citations to all articles, Anurag Acharya and...their own libraries and offering another way to consider and quantify the impact of scientific publications...

...exemplifies our long-standing commitment to be responsive to the community’s needs and our commitment to...many exciting things planned in 2023, from the expansion of our Data Hub to process improvements in every...

...ample resources and a positive perspective on the transition to a post-academic career. This training was ... inadequate job of supporting science trainee transition into diverse, successful careers.  We’re not ...

... check to make sure that it isn't methylation sensitive . Plasmids grown in Dam or Dcm methylase...opens in a new window) NEB's table of methylation sensitive restriction sites . Sometimes enzymes cut sequences...diagnostic digest), you can create a "Master Mix" consisting of all of the reaction components except for ...each tube. This will save you time and ensure consistency across the reactions....

...Directors brings human resources experience and insight to the now 13 member board at Addgene. Let’s meet...Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog...

... It is important to identify your audience and consider their background. Social media is a powerful tool...what you are trying to convey without having to extensively use Google search. Be careful about hidden connotations...

... another level with the addition of two light-sensitive ingredients - CRY2 and CIB1 – which they borrowed...advantages of the new system: "First, the light-responsive molecules of cryptochrome 2 are the chromophores...

...with cells that already have Cas9). Each library consists of approximately 3,000 gRNAs, as opposed to the...still offering the potential for true biological insight. Pooled and individual plasmid formats A first ...

...CRISPR-based technique to generate C->T or G->A transitions in a target sequence in live cells. This technique...endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by gRNAs. Caveats...

...delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages...for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the tetraloop of the...

...traction. One way to consider where the open access (OA) movement might be going is to consider some of the places...

... the spine. For binders of protocols and data, consider using color coded binders and tabs (for different...to protocols in real time. You may also want to consider moving your paper spreadsheets for reagent storage...

...1 mL). When working in a laboratory, properly dispensing liquid ensures the accuracy of your experiment...experiment and any changes to the amount you are dispensing can negatively impact your experimental results. ...the liquid and avoid touching the pipette to the inside of the tube. Only the tip should be in the liquid...different tubes or into wells of a microplate, you may consider using a multichannel pipette. Please watch Addgene...

...Foundation are used in Parkinson’s research.  The extensive resource from the Structural Genomics Consortium...Consortium enables science to determine the three dimensional structures of proteins.  The RESOLUTE Consortium...

... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

...at his University of Wisconsin lab for a summer during my PhD and is responsible for my enduring love ...

...cupcakes, or maybe the lab member originally responsible for the deposit had already moved on to their...with journal articles. But we do encourage you to consider sharing them openly as soon as that preprint is...

...researchers around the world. We take this responsibility seriously. Every decision we make, every project... provides unmatched customer service that is consistently timely, informative, friendly, and professional...

...generally follow the same pattern described above - a sensing domain induces changes in a fluorescent signal ...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...

...a separate plasmid. This system amplifies the intensity of the fluorescent signal and enables single molecule...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...

... vesicle reporters that are detectable by an inexpensive and widely available imaging platform: ultrasound... experiments because  ultrasound devices are inexpensive, widely available, and have deep tissue penetration...

...instead results in the breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

...a time. Just be sure there is a point person responsible for incorporating everyone’s suggestions into...timelines for experiments and manuscripts, who is responsible for which parts of the project, and, as mentioned...

...descriptions often fail to capture all of the responsibilities of a role, so it helps to see how people describe...path one you could see yourself taking? If so, consider setting up an informational interview with them...

...for our quality control process because of its expansive feature library and useful tools. In this blog...proteins that are already included in Snapgene’s extensive database. Snapgene also provides easy visualization...

...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ...Nature Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own...

...most applications. Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media...store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in ...

...important as its capabilities are the practical considerations of actually using it - the device has a rechargeable...expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain ...