We narrowed to 877 results for: HA

...PAM motif, averaging 19 targets per gene. The lab has previously used the library with SpdCas9 to perform...

...difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate...

...teams successes. “When PhD students graduate there has to be champagne, that's a given.” Furthermore, anyone...

...?” Some of the Tweeted comments: “A good mentor has your goals/needs/situation in mind, rather than (...

...datasets. It’s also a great place for anyone who has used IBEX and wants to preserve and share their validation...

...  While rapidly expanding our COVID-19 resources has been our focus for the past months, we’re excited...

...Minamimoto’s lab known as deschloroclozapine (DCZ) has been developed to meet these requirements. TL;DR...

...amplification are endA negative, meaning the gene has been removed/mutated to neutralize the nuclease activity...

...extra effort to make sure everyone in the meeting has an equal opportunity to contribute. Using video conferencing...

...Figure 3: Each publication featured in BioGRID ORCS has a dedicated summary page with PubMed identifiers ...

...soon as we become aware the related journal article has been published we’ll get the plasmids online for ...

...titers found in our in-stock preps. If a request has technical considerations that may affect expected...

...Old World monkeys it actually targets neurons, and has 45-fold higher expression levels compared to AAV9...

...enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration of small...

...large size: a 24x scaffold fully occupied with GFP has a molecular weight of 1,400 kDa vs 24 kDa for GFP...

...While AAP promotes virion assembly, the role of MAAP has not been clearly defined yet. Now you’ve got a basic...

...the cited papers to determine whether the plasmid has been used in your system. Sophisticated search You...

...biology tool, but like any biological system, it has certain drawbacks. First, the efficiency of Cre recombination...

...leadership. Perhaps one of your advisors or mentors has urged you to work on developing your “leadership ...

..., who went on to win the Nobel Prize. Dr. Doudna has had a host of other impressive mentors including ...

...happy to hear that I am not the only Addgenie who has a thrifty streak.  One of the most accessed blogs...

...to a variety of careers outside of academia. She has had previous graduate students and postdocs go on...

...complicated math. Bioengineering is a field that has been gaining a lot of traction in recent years. The... wealth of smaller engineered antibody fragments has further increased possibilities for synthetic antibody...

...writing, etc. Also, it is unlikely that a mentor has the only relevant expertise and perspective for your...been doing something all wrong or that a reagent has not been convincingly demonstrated to work for your...

...unexpected findings, or understanding why your experiment has not worked…again…for the seventh time….   I find ...

...Developmental Studies Hybridoma Bank, a nonprofit that has been supporting hybridoma sharing within the community...

...direct IHC, which uses just a primary antibody that has your reporter (in this case a fluorophore) directly...

...Benchling as an electronic lab notebook, it also has a suite of molecular biology tools and can make plasmid...

...be used in research or for therapeutics Addgene has compiled various educational resources to facilitate...

...your data for sharing on the site. The entry form has 5 general question types and it takes approximately...

...number of autophagosomes. This approach, however, has inherent limitations because it turns a dynamic process...

...the viral service in 2016 Addgene’s research team has been actively investigating ways to improve viral...

...eScience Labs. She still gets to write curriculum, but has taken on other new and exciting responsibilities....

...Iowa called “Teaching Your Research.” This course has participants take their research from the bench to...

...individuals through stock or investor pay outs. Addgene has no investors or stock. All of our proceeds go into...

... is easy, after all, who working in a laboratory has never faced an odd situation with experiments? Our...

...that the reader is not in their head. The scientist has to introduce the reader to the manuscript and engage...

...Donor DNA for Enhanced CRISPR Genome Editing CRISPR has revolutionized how researchers make knockouts and...

...virus on their own.Harvard researcher Connie Cepko has been helping us with AAV testing and recently had...

...The IgnaviCas9 protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is ...