We narrowed to 849 results for: HA

1. The Golden Stopwatch Award: Recognizing Facilitators of Research

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   ... Anyway?) Don't want to wait? Talk with your TTO Has a pending legal agreement ever delayed your access...

2. Fluorescent Biosensors for Measuring Autophagic Flux

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   ...number of autophagosomes. This approach, however, has inherent limitations because it turns a dynamic process...

3. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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   ...the viral service in 2016 Addgene’s research team has been actively investigating ways to improve viral...

4. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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   ...individuals through stock or investor pay outs. Addgene has no investors or stock. All of our proceeds go into...

5. Bricking Science: Portraying Scientific Reality Through LEGO

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   ... is easy, after all, who working in a laboratory has never faced an odd situation with experiments? Our...

6. Tips for Improving Your Next Manuscript

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   ...that the reader is not in their head. The scientist has to introduce the reader to the manuscript and engage...

7. 10 Great Guest Posts We're Thankful For

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   ...Donor DNA for Enhanced CRISPR Genome Editing CRISPR has revolutionized how researchers make knockouts and...

8. Using Addgene's New Viral Service for Your Research

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   ...virus on their own.Harvard researcher Connie Cepko has been helping us with AAV testing and recently had...

9. Addgene Begins Distribution of Recombinant Antibodies

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   ...Developmental Studies Hybridoma Bank, a nonprofit that has been supporting hybridoma sharing within the community...

10. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    ...direct IHC, which uses just a primary antibody that has your reporter (in this case a fluorophore) directly...

11. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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    ...Benchling as an electronic lab notebook, it also has a suite of molecular biology tools and can make plasmid...

12. Summer SciComm: Science Storytelling

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    ...unexpected findings, or understanding why your experiment has not worked…again…for the seventh time….   I find ...

13. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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    ...eScience Labs. She still gets to write curriculum, but has taken on other new and exciting responsibilities....

14. Oh, The Places You Can Go: Careers in Science Communication - Product Development

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    ...Iowa called “Teaching Your Research.” This course has participants take their research from the bench to...

15. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    ...The IgnaviCas9 protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is ...

16. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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    ... Thus far being an Outreach Scientist at Addgene has been awesome. The process of writing, editing, and...

17. Management for Scientists: What Makes a Good Manager Anyway?

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    ...decisions, assignments and polices. Sometimes the boss has to be the boss and make difficult and “unpopular”...

18. Plasmids 101: Antibiotic Resistance Genes

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    ...In these instances, the cloning vector typically has two separate resistance cassettes and your gene of...

19. Cancer and the Immune System: Deciphering the Relationship

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    ...possible angle to leave no stone unturned, but cancer has been producing landslide after landslide, only adding...used immunotherapeutic agents. Currently the FDA has 12 approved monoclonal antibodies targeting the PD-L1...

20. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ...regions of the gene is also useful in case the gene has a weird mutation that you didn’t know about (which...and is a testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months...

21. Plasmids 101: Origin of Replication

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    ... proteins, respectively. A plasmid's copy number has to do with the balance between positive and negative...

22. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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    ... interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation...

23. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    ...quantified at 595 nm. This method is time consuming and has low sensitivity so it’s not used much. However, the...

24. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    ...2019.  Wensheng Wei's lab at Peking University has developed a new CRISPR screening method that makes...

25. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    ... AAV quality control Historically, AAV vector QC has been sparse including only titration of AAV particles...

26. New and Upcoming Viral Vectors - September 2019

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    ...fluorescence from inactive neurons is reduced. This variant has an approximately 12-fold increase in brightness after...

27. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ...proteins (Natsume et al., 2016). Now, the Kanemaki lab has created a new toolkit of plasmids for the N- or C...

28. Visualizing Translation at the Single Molecule Level

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    ... disrupting ribosome-mRNA complexes. Recent work has adapted this technique to monitor local translation...

29. The Power Behind NGS Plasmid Validation: seqWell

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    ...with our plasmid assembly pipeline. To date, this has resulted in high quality NGS-based assemblies for...

30. "Build Your Plasmid": The Game - Play to Teach

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    ... As I wrote in the blog post, BUILD YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based...

31. In Vivo Biotinylation of Bacterial Fusion Proteins

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    ... binding protein (MBP, New England BioLabs). MBP has a similar function as GST in that it provides a method...

32. Revamp Your Lab Meetings With Creative Virtual Collaboration

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    ...industry”. To date, aside from doing science, he has assisted many researchers in gaining the confidence...

33. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    ...considerations of actually using it - the device has a rechargeable battery, is relatively small, and ...

34. Trading Spaces - Visiting Scientist Edition

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    ... few labs have expertise in. Visiting a lab that has this expertise will provide you with guidance and...

35. Antibodies 101: Chimeric Antibodies

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    ...imaging experiment   We hope that this blog post has answered all of your questions about the utility ...

36. Plasmid Cloning by PCR

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    ...plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to...

37. Don’t Wait To Launch Your Health Science Startup

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    ...this deter your ambition. If you believe your idea has potential to make a difference, then make sure you...

38. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    ...Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now, one ...

39. Management for Scientists: Delegating is Key

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    ...individual who is a local expert in that task (or has the bandwidth to become an expert) the end result...

40. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...protein, if you know that your protein of interest has a similar spectrum. For example, here's a screenshot...