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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 919 results for: IRE;

Showing: 681 - 720 of 919 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,..., M. D., Wu, J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-dependent GPCR activity...

  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...in the depositing lab, although you may have an entirely different use in mind! This section of our plasmid...in the E. coli lab strain DH5a, but others may require strains such as ccdB survival (for Gateway plasmids...

  5. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...

  7. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...choose, you may not be able to elute the antibodies directly into your preferred buffer. If that’s the case...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

  9. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...impacted the experiments we wanted to perform. It required a great deal of time and effort to test and optimize...J. O., Outram, S., Koenig, B. A., Pereira, S., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives...

  10. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...availability following cross-linking fixation; they require an additional step generally referred to as Antigen...retrieval here because it’s not quite as ubiquitously required for IF as fixing and permeabilizing.  References...

  13. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...the frontiers of fluorescence microscopy often requires more photons. For example, moving from transient...Unlike other systems, no genetically encoded tag is required as the small-molecule binding motif targets endogenous...

  14. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats...integrase-deficient lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector...

  15. CRISPR Antimicrobials

    Type
    Blog Post
    ... PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular...

  17. Lab to Office Culture Shock

    Type
    Blog Post
    ...process of onboarding a new employee who's coming directly from grad school. The transition has been a bit...thought they would be working in a lab for their entire career. Yet even success in academia means you’...

  18. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... with a distributor for that country. In China, direct shipments weren’t able to clear import control...., we launched the Addgene blog for topics that required a lot of nuance or detail. By the end of 2023,...

  19. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...as Rep or other initiator proteins, they do not require host proteins for replication and can maintain ...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...

  20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...SaCas9, Zhang’s group used an approach called BLESS (direct in situ breaks labeling, enrichment on streptavidin...

  21. How to Be an Excellent Trainee

    Type
    Blog Post
    ...someone who provides career advice and offers direction on your scientific project. Your scientific mentor...meaningful relationships in your new lab, learn the required techniques, and be ready to take on trainees one...

  23. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...about its limited resources. Rather than developing entire new teams in house to solve every problem (although...collaborators’ professional developments without directly contacting them. One great way to transition from...

  24. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...solution. Since this technique is intensive and requires an electron microscope, it is not routinely performed...a few ways:  Absolute quantification by qPCR requires generating a standard curve of known concentration...

  25. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...all of these platforms were developing, Google acquired Youtube and has been growing its online video ..., also known as the science videographer, is a retired botanist and oceanographer who has a blog and several...

  27. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...course of infection. These binding events can be impaired by the electrostatic repulsion of the negatively...

  30. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...electron microscopy (cryo-EM) protocols typically require high concentration and purity of the target molecule...particles (eVLPs) offers an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain no...

  31. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...forward scatter (FSC) and side scatter (SSC). To acquire these measurements, the cytometer illuminates the... “lymphocyte + single cell + live” population, acquired from the previous gating pipeline, about 53% of...

  32. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...., Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and optogenetics of Rho GTPases...Piatkevich, K.D. et al. A robotic multidimensional directed evolution approach applied to fluorescent voltage...

  33. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...to support your work. For example, if your work requires specialized equipment, try finding institutes ... institutes were able to provide the resources required for him to meet his goals. Therefore, he turned...

  34. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Similarly, placing UAS upstream of a transgene permits directed expression of that gene in cells that also express...systems, allowing for more complex control over desired gene expression. Take a look at all of the options...

  37. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...cell that gets the plasmid contains all of the desired gRNAs. This increases the chance that all the edits...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo Lab multiplexed...

  38. Deep Dive: qPCR

    Type
    Blog Post
    ... you to measure the intensity of signal over an entire cycle. Since signals are produced by the presence... practice, multiplexing is quite difficult and requires significant optimization to ensure the results...
Showing: 681 - 720 of 919 results