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We narrowed to 1,006 results for: RING

Showing: 681 - 720 of 1006 results

  2. Science as Art

    Type
    Blog Post
    ...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and art was Albert G...smaller competitions are now taking place and gathering momentum, including in Cambridge and Bristol. ...

  4. What's New in CRISPR - March 2020

    Type
    Blog Post
    .... Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor plasmids Cytosine...RNA-guided methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine...

  5. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...the sensitivity of nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with.... (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo. Nature Methods, 20(9)...

  8. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...the AAV p40 promoter. Alternative splicing and differing translational start sites generate the three VP...ratio (VP1:VP2:VP3). There are eleven naturally occurring AAV serotypes that have thus far been identified...

  10. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...reporter plasmids, viral plasmids, and genome engineering plasmids. Some of the many things that plasmids...controlled changes to an organism’s genome (genome engineering) Produce synthetic viruses that can be used in...

  11. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ... organoids derived from other tissue-types. By bringing together CRISPR-based approaches and organoid ...the fluorescence intensity of the cpGFP without altering that of the internal control (Fig 1). Because ...

  12. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...any paper at all. Posting preprints and openly sharing materials are both excellent ways to provide transparency... articles. But we do encourage you to consider sharing them openly as soon as that preprint is online!...

  16. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ... online course had three aspects to it: videos starring Roberto Kolter, webinars taught by Peggy Cotter...the online class, we met as a group for sessions covering individual portions of the manuscript. Stephen...

  17. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... science project started when I realized I was pouring large agarose gels for DNA visualization or isolation... had to be careful not to stick your fingers in during a run or risk minor electrocution.  These days ...

  19. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... high G+C content genes, as validated through monitoring the expression of fluorescent proteins and oxytetracycline...-nucleotide modifications to edit DNA without requiring a double-stranded break. The Xiao Wang Lab has...

  22. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...infection, that is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate...and Man: A History of Interactions (pp. 23–40). Springer International Publishing. https://doi.org/10.1007...

  23. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...transduction?  Transduction is the process of transferring foreign nucleic acid into a cell by a viral ...Expressed episomally long-term? Expressed short-term? Answering these questions will help guide you to the right...

  25. The Strength of Story Telling

    Type
    Blog Post
    ...talks and interviews. They even tell short stories during weekly lab meetings. When scientists tell good ...when appropriate. It might not be scientific to bring emotional discovery into a scientific story, but...

  28. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...therefore titer may not need to be as high when delivering receptor constructs with AAV. In the case of ...less particles to be released into suspension and lowering the number of viral particles that can be harvested...

  29. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...

  30. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...multiplex strategies have been facing difficulties in pairing with base editing and prime editing approaches....also enable more convenient multiplex genome engineering of crops or allow for sophisticated CRISPR screenings...

  32. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... ITR integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth...help to reduce the likelihood of ITR deletions occurring.  How do I validate my ITR sequences?  Next-generation...

  33. How to Lead a Great Meeting

    Type
    Blog Post
    ...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...review the agenda and add anything that is missing.  During the meeting, take notes on the agenda points. At...

  34. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...delegating for success.  Meet regularly before during and after the assignment to discuss goals, expected...the Management for Scientists Series Learn How Volunteering at a Professional Organization Can Help You ...

  36. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...GFP is often used as a reporter and we will be covering this ever-versatile fluorophore in a later post...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working...

  37. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...come up with a new solution.  Now, you may be wondering how this all fits in with science. Well, I’ll ...a software product manager does, you might be wondering what the day to day looks like. Check out the ...

  38. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor 44361 AAV1...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  39. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light...  Mary Gearing...
Showing: 681 - 720 of 1006 results