...al., 2022). B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before...

...tags whose localization you can monitor in live cells (beware of artifacts!), photoactivatable tags for...Purification Sometimes looking at a protein inside cells just isn’t enough to get all the information you... tell you much about its function and/or alter cellular activity in a way that’s interesting from an applied...expression and activity thereby limiting the use of cellular resources required for the production of large...small molecules, light, temperature, or even intracellular metabolite levels. Different plasmids and, often...

...daughter cells. These daughter cells contain the same genetic information as the parental cell, and are...with the host cell. Like the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division, and...and each daughter cell receives at least one copy of the plasmid. By the 1970s the combined discoveries...are thus termed clones of the original cell. The plasmid DNA is similarly referred to as cloned DNA, and... and ideally would replicate these plasmids upon cell division in addition to their own genomic DNA. Because...additional replication burden, the rate of bacterial cell division is reduced (i.e., it takes more time to...against the propagation of these plasmids through cell division. To ensure the retention of plasmid DNA...

.... Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter...

...fellow at Vanderbilt University. She studies beta-cell function and failure in Type 2 Diabetes. Birmingham...BC (Canada). She works in a lab that uses yeast cells to study how DNA damage promotes cancer. She is ...

...Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function. Cell Stem Cell. 28:112-126...targeted to specific tissues, cell types or even subcellular regions of a neuron. Cell-specific expression of...actuators for specific cellular pathways, receptors, or ion channels targeted to specific cell populations (most...signaling, therefore allowing precise control over the cell population they are targeted to. To find chemogenetics...coupled receptors, or GPCRs. The largest class of cell surface receptors, GPCRs are seven-pass transmembrane...Common promoters in chemogenetics plasmids Promoter Cell Specificity hSyn1, CaMKIIa Neurons GFAP Glia CD68... AAV-delivered constructs can be controlled with cell-type specific promoters. Table 4 lists some common...

...and chemically excited a class of enteroendocrine cells within the intestines known as neuropods. They observed...

...Associate at Harvard Medical School. He manages the Cell Biology Microscopy Facility, specializing in advanced...  There are many online guides – including an excellent series of posts by Joachim Goedhart on the Addgene...

... Eye Institute and is particularly interested in cell signaling.     Additional Resources on the Addgene...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently...

..., and also a Professor of Molecular Genetics and Cell Biology at the University of Chicago. He was an ...

...efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321...

...recommend validating the serotype in your target cells before conducting the experiment. Likewise, a lot...

... small amount of spurious DNA from the packaging cell line, helper and packaging plasmids. We decided ...

...example, several journals like eLife, Nature, and Cell use  Research Resource identifiers (RRIDs) which...

...sciences, viewed through the lens of development, cell, and molecular biology. Beyond that, she is also...

... Transform TOPO Cloning Reaction into Competent Cells: You can use your standard lab protocol for this...

...systems may prove effective in other species or in cell culture. In addition to CRISPR/Cas9 applications...

...devise new strategies to reproduce within a host cell. A few virus particles have learned to circumvent...

...subpopulation of cells within the injection site of the viral vector, rather than in all cells. For example...2010 Molecular and cellular approaches for diversifying and extending optogenetics. Cell. 196:1-28. PMID ...and genetic engineering to measure and manipulate cells (frequently neurons) and their governing biomolecular...detect, measure, and control molecular signals and cells in order to understand their functions. Example ...wavelength, the pore opens, cations flow into the cell (yellow dots), and the neuron is activated. Optogenetics...which conducts the flow of ions into or out of the cell. Scientists have identified a variety of naturally...adding trafficking signals to localize opsins to the cell membrane. Microbial opsins, such as those described...

...team potential. I often thought that performing a cell culture experiment myself would take less time than...Another team member was highly self-motivated and excelled when given the freedom to explore projects autonomously...

...spend the afternoon lovingly tending to the stem cells I was using in an experiment, then spend the evening...

... most relevant to plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry...

...day was Julie from Novartis who was working with cell culture and who was extremely helpful since she ...

...modification may not be easily expressed in non-mammalian cells. Yeast expression plasmids The yeast expression ...

...manuscripts submitted for publication. Holds a PhD in Cell Biology and a Masters degree in Infection and Immunity...

...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura...efficiency and are important for robust activity in cells (Jiang & Doudna, 2017). Within an interior cavity...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...

...receptors to selectively control and investigate cell signaling pathways. Receptors are remotely controlled...and genetic engineering to measure and manipulate cells (frequently neurons) and their governing biomolecular...detect, measure, and control molecular signals and cells in order to understand their functions. Read More...

...Lacy was also employed by the American Society for Cell Biology in a position working with preprints in ...

...measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the...

...industrial or research purposes requires stable host cells (the simplest example would be a transformed mesophilic...

...CRISPR-Cas12b for mammalian genome engineering." Cell discovery 4.1 (2018): 63. PubMed PMID: 30510770....

...Science Communication” at the American Society for Cell Biology (ASCB) 2018 Conference. I don’t exactly ...

...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...

...describes the specifics of these regions in eukaryotic cells. Core Promoter The core promoter region is located...bind. Histones are proteins found in eukaryotic cells that package DNA into nucleosomes. Histone binding...element controls the rate of transcription. Bacterial cells contain sigma factors which assist the RNA polymerase...ribosomal RNA (rRNA) which is a main component of a cell’s ribosome structure. Ribosomes are the site of protein...

...Facebook and Gmail, were launched. And Melina Fan, a cell biology graduate student, was frustrated by the ...to receive the Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also ...

...fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016...

...protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what...better than a nitrocellulose membrane. If you have already run your blot using nitrocellulose, you may decide...instructions for the membrane you are using. Dry nitrocellulose membranes are brittle, so proceed with caution...

... a journal club to my molecular biology class. A Cell paper. With six figures. Averaging ten panels each...

...This can be pretty complicated given that a single cell has tens of thousands of proteins functioning in...

...immunoassays in DNA damage and instability detection. Cell. Mol. Life Sci. 76, 4689–4704 (2019). https://doi.org...