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  1. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools for targeted...nuclease-competent Cas9, obviating the need to generate new cell lines or transgenic animals expressing dCas9 as a means...

  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ... the hierarchy of web pages the plasmid page is nested in. You can see in the example above that the bread...Database has more information about common vector backbones.  The backbone modifications listed can refer ...

  3. MXS Chaining

    Type
    Blog Post
    ...derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin ... might not, however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation...

  4. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...protocols and examples of applications from across disciplines now available. In this post, we’ll review the...example, cytoskeletal structures that form continuous lines in pre-expanded samples can sometimes take on a ...

  7. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available. Labs with limited...reader as opposed to chemifluorescent, and chemiluminescent methods that require specialized equipment...

  9. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...Other fields, often highly technical or skills-based ones, may not use them very often. Similarly, entry-level... cover letter should be no more than a page. Conciseness is key! If possible, address to the professor...

  10. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...easier. You should also demonstrate a strong willingness to help others with their problems, a general...sorts of details about the application, just not the ones that matter. Therefore, if there is a bit of a Sherlock...

  11. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... than optimal user experience and the overall soundness of the software design. Each of us brings slightly...Demo details for this would be something along the lines of showing that the checkout takes the user directly...

  12. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...base pairs long and found upstream of their target genes. The sequence of the promoter region controls the...elements, insulators, and silencers; however, some "leakiness" of transcription may occur. This is normally ...

  13. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...talks for non-academic settings. Many of these guidelines will apply to any type of science presentation...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared...

  15. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...the scientists and scientific process behind the scenes to the new antibodies and research coming out of...

  16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...illumination, substantial PA accumulates in the plasma membranes, indicated by the PA probe colocalizing with SuperPLDx30...

  17. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...rules) to some volunteers, who filmed with their phones. Test: I loved the end result, but it was clear....  Build: If you’ve ever watched any behind the scenes footage in the last several years, you can usually...

  21. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...amino acid peptide contains a cluster of four cysteines (CCXXCC, where X is any non-cysteine amino acid...the fluorophore’s microenvironment to enhance brightness, stability, or other properties is an active ...

  23. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...Statistics – The basics Statistical analysis is what determines if there is statistical significance among data...There are many, many statistical tests beyond the ones detailed in this article. These are just some of...

  27. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...above 30% for a variety of targets in human cell lines, with an average indel frequency of only 1.1%. These...which is significantly more effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following...

  29. 2022 Depositor Week

    Type
    Blog Post
    ...this is it!)  Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors Hall of Fame  Thursday...

  32. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...focus on generating knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll...
Showing: 681 - 720 of 905 results