We narrowed to 954 results for: App;

1. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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   ...a handle on some of these mysteries. Another application is clinical; can we find ways to treat patients...

2. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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   ...you choose could be based on your downstream application, time, and instrument availability. UV absorbance...

3. Plasmids 101: Gram Negative and Positive Bacteria

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   ... added, it dehydrates the peptidoglycan layer, trapping the large dye complex created by the interaction...

4. New and Upcoming Viral Vectors - June 2019

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   ...ligand-gated ion channels that can be activated with FDA-approved drugs. See our inventory of DREADDs / Chemogenetics...

5. Visualizing Genomic Loci with CRISPR-Sirius

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   ...has been widely adapted for a wide variety of applications: base editing, CRISPR activation and inhibition...

6. Creating accessible biology activities in schools with BioBits

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   ...mix chemicals together in chemistry to see what happens, but there isn’t really an equivalent of a “chemistry...

7. Viral Vectors 101: Inverted Terminal Repeats

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   ... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and...

8. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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   ...by TUNEL staining.  The video below shows what happens to the zebrafish’s heartbeat and blood circulation...

9. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   ...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...

10. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    ... an upright position for 30 seconds, and then recapping the tubes prior to thaw. We compared vented and...

11. The Power Behind NGS Plasmid Validation: seqWell

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    ... we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation Technology for...

12. Cultivating Community Science at BosLab

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    ...the biotech revolution. Our lab exists in what appears to be a funky old garage, but looks can be deceiving...

13. With Our New Viral Service, We're Taking CRISPR Further!

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    ...Numbers in the large colored circles are rough approximations of the total number of CRISPR plasmids for ...

14. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    ...The LipoGlo system represents a new, intriguing approach to expand our understanding of ApoB-LPs and accelerate...

15. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...

16. A Quick Guide to a Career in Software Product Management

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    ...realize that the bench isn’t where they will be happiest. What is software product management, and how ...

17. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    ...other ways to choose restriction enzymes for this approach. If you’re planning to go this route, check out...

18. Building and Validating Recombinant Antibodies: A Case Study

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    ...antibodies that are specific to each subclass. Approximately 70% of mouse mAbs are IgG1 subclass (Manning...

19. Antibodies 101: Buffers, Storage, and Conjugates

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    ... to be stored either in a dark, opaque vial or wrapped in aluminum foil to prevent ambient light from ...

20. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    ...Scientists can also use electroporation, the application of an electrical charge to cells, to increase...

21. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...

22. 15 Years of Addgene: The Top 15 Plasmids

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    ...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...

23. Early Career Researcher Toolbox: Social Media for Scientists

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    ...s advice to tap into the online conversations happening around a scientific conference, whether you are...

24. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    ...discussed in this post, voltage has been adjusted appropriately and compensation performed. Before you read ...

25. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...

26. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    ... markers make it easy to spot flies with the appropriate transgene. One such marker is the white gene,...

27. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    ...interactions, cancer immunotherapy, as well as new approaches to reprogramming tumor-associated inflammation...

28. Improved Plasmid Maps Powered by SnapGene

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    ...can be detected is 5x greater than our previous mapping software and now includes Type IIS restriction ...

29. Screening for Successful Genome Editing with Digital PCR

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    ...a gene of interest is one of the most common applications of precision genome editing, the remainder of...

30. Top Requested Lentivirus and AAV of 2016

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    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...

31. How to Make Friends and Meet People at a Scientific Conference

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    ...relationships that are memorable. It isn’t always appropriate to walk up to someone much more senior than you...

32. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    ...take place in a tissue culture dish but can also happen in real-time inside a mouse’s brain.  But the brain...

33. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    ...the skin making it important to study what is happening within the keratinocytes. “We hope to use CRISPR...

34. RNA Extraction Without A Kit

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    ...potentially complicate results of a downstream application such as quantitative PCR to assess gene expression...

35. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    ... cells to the microenvironment. This mechanism happens for both short and long distances. When it comes...

36. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    ... assays Long-lived protein degradation: This approach measures the degradation rate of radio-labeled...

37. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    ...densely populated areas. This assumption does not appear to hold. There are institutions in Boston - Massachusetts...

38. Addgene's Tips for Plasmid Quality Control

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    ... glycerol stocks, so make sure to select the appropriate service and read the submission instructions....

39. The Future of Research Symposium Boston 2015

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    ...BEST) awards, which are designed to support new approaches that address the need to train our young scientists...

40. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ...there’s a lot of control spatially as to what’s happening at different points within the wood. There are...

41. Career Coaching for Scientists: Why and Where Do I Find One?

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    ...Industry Professionals”. This larger shop might appeal to some although most scientist coaches can help...

42. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...MiSeq and set up the run making sure to note the appropriate barcode for each sample. About 24 hours later...

43. Hot Plasmids and Viral Preps - March 2021

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    ...from Tang et al., 2020.   G-baToN can also be applied to other fluorophore/antibody pairs. Because the...

44. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    ...choose essentially any background, but two common approaches are either a solid color, or a “themed” background...

45. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: 21994751. PubMed...

46. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ... also used to teach students about restriction mapping and western blotting. Students who participate ...

47. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...influence (non-homologous end joining, or NHEJ), in approximately one percent of cases, the stem cell will seek...

48. Hot Plasmids and Viral Preps - January 2021

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    ...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...

49. Illuminating Epigenetics with A FRET Based Biosensor

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    ...risk of dangerous DNA damage. If the way DNA is wrapped around histones changes, then gene expression can...

50. Gaining Leadership Skills Volunteering at a Professional Organization

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    ...institutions are not local but are national issues. I applied and was successfully granted a travel award to ...