We narrowed to 1,017 results for: PLE

...for compliance. However, 10 days prior to the implementation date, our data indicated that, while 69% of...conditions for junior scientists but are not implementing consensus recommendations, even when a federal...

... not all grant duties to trusted students. For example, if the proposal call aligns with your work, your...Below are the typical sections in a NIH RFA, for example, and tips to consider while writing each section...

...treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster and Harlan...female mouse for gestation. All three can introduce multiple base pair deletions, but CRISPR/Cas9 can also ...

...of science, even while I was a bench scientist completing my graduate studies in molecular biology and ...academic writing and presentations. But for many people who don’t work in these fields of research, what...

...of synthetic promoters were also used to target multiple cell types and could also be used for sparse labeling...While tested in retinal cells, the same design principles could be used to create synthetic promoters for...

...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ...Overcoming current limitations to permit the use of multiple FRET biosensors simultaneously would further increase...

...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...varies widely with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First...

.... I can support that.” That was interesting. If people could be against GMOs as food but support GMOs ... the day. It would be a beautiful GMO, one that people would actually want to have. Once we had the idea...

...specific epitope that your antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le...be the antibody Mary Poppins!   All of the examples of new versions of Myc1-9E10 mentioned above basically...

... is reported to target skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies...  Multiple Authors...

...known tropism for your cells of interest — for example, AAV8 for kidney cells — can help improve infection... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

...functions in use But exactly what kind of editing are people doing with all these CRISPR plasmids (and libraries...these categories to see what kinds of CRISPR tools people were depositing and requesting each year (Figure...

...transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed...transcribed gRNA are combined to form a Cas9-gRNA complex and delivered to cells using cationic lipids. Transient...

...producer cells where there is E1 and E3 gene complementation. In target cells that are infected by the vector...Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/...

...between Jan. 1 and Mar. 1, 2020. Figure 2: A simple approximation of the effect of COVID-19 on data...editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered in his laboratory. Learn...

...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...

...range of issues related to AAV vectors, many from people trying to answer the exact same question I was:...Sharing data and community concerns AAV vectors are complex biological reagents; many factors, like storage...

...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

... own proteins, making the purification process simpler. Due to its high yields, E. coli is often used ... the purity of your antibody prep. If you have multiple contaminating bands or a few strong ones, consider...

...PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage at the target site until either...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...

... committed to understanding some of the basic principles of life and health and works to advance foundational...enhancer sequence, then interact with mediator complexes and the RNA polymerase at the promoter region ...

...incompatible? Plasmid incompatibility occurs when multiple plasmids within one cell have the same replicon...Schumacher, 2012). This concept is a little more complex than simply competing for replication control factors...

...done by a single enzyme; however, eukaryotes have muliple polymerases which are each responsible for a specific...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells...

...powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can...Howard M. Salis. "Translation rate is controlled by coupled trade-offs between site accessibility, selective...

...have various disadvantages and limitations – for example, microtubule-destabilizing drugs are impossible... embryo (Aljiboury et al., 2023). It’s a great example of how optogenetics allows you to disrupt and study...

...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...BL21 strain and derivatives are the most common examples of the E. coli B strain. Common E. coli strains...

...modifications. CRISPR base editing persists through multiple cell divisions, indicating that this method produces... Multiple Authors...

...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

... you don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to ...

...BioRender.com. Figure 1: PRIME editing Please check out the updated guide for a great introduction...

...executing, and troubleshooting your experiments. For example, if you’re very new to antibodies as research tools...

...  Multiple Authors...

...sharing In a recent review on CRISPR technology, for example, the authors Patrick Hsu, Eric Lander, and Feng...

... first paper came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to ...

...the twelfth day of CRISPR, Addgene gave to me: multiplexed gRNAs.  We hope you’ve been enjoying our CRISPR...

...Ahem, ahem, drum roll please! It's time to announce the winners of our 2021 Deck the Lab Contest! Though...

...’s Academic and career goals. Career advice for people going into academia: follow your passion and pay...

...biological research and discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 ...

...divided into three main sections: Protocols To complement our written protocol materials, we have also ...

...repository  IBEX Knowledge-Base  Any IBEX multiplex tissue imaging Data repository See blog post...

...single gene) 26:00-28:39 : Methods for Delivering Multiple Genes to Cells for Gene Therapy 28:40-35:10 : ...

...a TMPRSS2 plasmid from Roger Reeve’s lab as an example). Depositor Week will be fun for everyone, whether...

...imagination run loose on the lab. Have fun! Here’s an example from last year’s contest:   🎶Walking in a Winter...

...This calculator can do the math for you and it’s simple to make adjustments for the type of tissue culture...

...Kit from Markus Ralser's Lab -  Use this kit to complement unused auxotrophies in Saccharomyces strains....

... plastic and you have to share 1 trophy among 6 people, but you’ve earned bragging rights for the next...

...community is your negative data. We want to make sure people are selecting AAVs that will work for their experiments...

...bacteria, fungi, and viruses. Final tree with the complete #MicrobeAdventCalendar ornament set (plus more...

... to the needs of the scientific community. For example, we started a new service distributing ready-to-use...

... Multiple Authors...