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  2. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post
    ...treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster and Harlan...female mouse for gestation. All three can introduce multiple base pair deletions, but CRISPR/Cas9 can also ...

  3. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post
    ...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ...Overcoming current limitations to permit the use of multiple FRET biosensors simultaneously would further increase...

  4. Engaging with science and society at pgEd

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    Blog Post
    ...of science, even while I was a bench scientist completing my graduate studies in molecular biology and ...academic writing and presentations. But for many people who don’t work in these fields of research, what...

  5. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post
    ...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...varies widely with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First...

  7. Of Myc and Men

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    Blog Post
    ...specific epitope that your antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le...be the antibody Mary Poppins!   All of the examples of new versions of Myc1-9E10 mentioned above basically...

  8. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post
    ...functions in use But exactly what kind of editing are people doing with all these CRISPR plasmids (and libraries...these categories to see what kinds of CRISPR tools people were depositing and requesting each year (Figure...

  9. The AAVantages of AAV in CRISPR Screens

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    Blog Post
    ...known tropism for your cells of interest — for example, AAV8 for kidney cells — can help improve infection... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

  12. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post
    ...PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage at the target site until either...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...

  13. Uncovering Ancestral Bias in CRISPR Research

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    Blog Post
    ...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...

  14. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post
    ...range of issues related to AAV vectors, many from people trying to answer the exact same question I was:...Sharing data and community concerns AAV vectors are complex biological reagents; many factors, like storage...

  15. Antibodies 101: Producing Recombinant Antibodies

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    Blog Post
    ... own proteins, making the purification process simpler. Due to its high yields, E. coli is often used ... the purity of your antibody prep. If you have multiple contaminating bands or a few strong ones, consider...

  16. AAVs in Retinal Gene Therapy

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    Blog Post
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

  17. An Introduction to Adenovirus

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    Blog Post
    ...producer cells where there is E1 and E3 gene complementation. In target cells that are infected by the vector...Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/...

  18. Plasmids 101: Plasmid Incompatibility

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    Blog Post
    ...incompatible? Plasmid incompatibility occurs when multiple plasmids within one cell have the same replicon...Schumacher, 2012). This concept is a little more complex than simply competing for replication control factors...

  19. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post
    ...done by a single enzyme; however, eukaryotes have muliple polymerases which are each responsible for a specific...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells...

  20. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can...Howard M. Salis. "Translation rate is controlled by coupled trade-offs between site accessibility, selective...

  21. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post
    ...have various disadvantages and limitations – for example, microtubule-destabilizing drugs are impossible... embryo (Aljiboury et al., 2023). It’s a great example of how optogenetics allows you to disrupt and study...

  22. Plasmids 101: Common Lab E. coli Strains

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    Blog Post
    ...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...BL21 strain and derivatives are the most common examples of the E. coli B strain. Common E. coli strains...

  24. New Coomassie Protocol Video

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    Blog Post
    ...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

  25. Plasmids 101 eBook 4th Edition

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    Blog Post
    ... you don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to ...

  26. CRISPR Guide Update

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    ...BioRender.com. Figure 1: PRIME editing Please check out the updated guide for a great introduction...

  30. We’re Going Viral This Week!

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    ... first paper came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to ...

  31. 2021 Deck the Lab Winners!

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    ...Ahem, ahem, drum roll please! It's time to announce the winners of our 2021 Deck the Lab Contest! Though...

  32. The Twelve Days of CRISPR

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    ...the twelfth day of CRISPR, Addgene gave to me: multiplexed gRNAs.  We hope you’ve been enjoying our CRISPR...

  42. Countdown to Halloween @Addgene

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    Blog Post
    ... plastic and you have to share 1 trophy among 6 people, but you’ve earned bragging rights for the next...

  43. Newly Updated AAV Data Hub!

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    ...community is your negative data. We want to make sure people are selecting AAVs that will work for their experiments...

  45. Editor's Choice, October 2016

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    Blog Post
    ...Editor's Choice Blog Posts Last month there was plenty of excitement at Addgene. Our new viral service...

  48. Editor's Choice, July 2016

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    ...useful in FRET experiments. Professor Fessenden's example of a FRET experiment with all relevent controls...
Showing: 851 - 900 of 1011 results