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We narrowed to 1,018 results for: PLE

Showing: 851 - 900 of 1018 results

  1. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...several different species are present. One common example is using pre-adsorbed secondary antibodies (which... other antibodies) in experiments that require multiple antibodies that each detect a different antigen...

  2. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific... observation of individual endogenous protein complexes in situ by proximity ligation." Nature methods...

  4. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ... not all grant duties to trusted students. For example, if the proposal call aligns with your work, your...Below are the typical sections in a NIH RFA, for example, and tips to consider while writing each section...

  5. Engaging with science and society at pgEd

    Type
    Blog Post
    ...of science, even while I was a bench scientist completing my graduate studies in molecular biology and ...academic writing and presentations. But for many people who don’t work in these fields of research, what...

  6. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ...Overcoming current limitations to permit the use of multiple FRET biosensors simultaneously would further increase...

  7. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster and Harlan...female mouse for gestation. All three can introduce multiple base pair deletions, but CRISPR/Cas9 can also ...

  9. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...varies widely with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First...

  11. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... is reported to target skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies...  Multiple Authors...

  12. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...known tropism for your cells of interest — for example, AAV8 for kidney cells — can help improve infection... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

  13. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...functions in use But exactly what kind of editing are people doing with all these CRISPR plasmids (and libraries...these categories to see what kinds of CRISPR tools people were depositing and requesting each year (Figure...

  14. Of Myc and Men

    Type
    Blog Post
    ...specific epitope that your antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le...be the antibody Mary Poppins!   All of the examples of new versions of Myc1-9E10 mentioned above basically...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... own proteins, making the purification process simpler. Due to its high yields, E. coli is often used ... the purity of your antibody prep. If you have multiple contaminating bands or a few strong ones, consider...

  17. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

  18. An Introduction to Adenovirus

    Type
    Blog Post
    ...producer cells where there is E1 and E3 gene complementation. In target cells that are infected by the vector...Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/...

  19. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage at the target site until either...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...

  20. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...

  22. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...range of issues related to AAV vectors, many from people trying to answer the exact same question I was:...Sharing data and community concerns AAV vectors are complex biological reagents; many factors, like storage...

  23. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...incompatible? Plasmid incompatibility occurs when multiple plasmids within one cell have the same replicon...Schumacher, 2012). This concept is a little more complex than simply competing for replication control factors...

  24. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...done by a single enzyme; however, eukaryotes have muliple polymerases which are each responsible for a specific...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells...

  25. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...have various disadvantages and limitations – for example, microtubule-destabilizing drugs are impossible... embryo (Aljiboury et al., 2023). It’s a great example of how optogenetics allows you to disrupt and study...

  26. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can...Howard M. Salis. "Translation rate is controlled by coupled trade-offs between site accessibility, selective...

  27. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...BL21 strain and derivatives are the most common examples of the E. coli B strain. Common E. coli strains...

  29. New Coomassie Protocol Video

    Type
    Blog Post
    ...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

  30. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ... you don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to ...

  31. CRISPR Guide Update

    Type
    Blog Post
    ...BioRender.com. Figure 1: PRIME editing Please check out the updated guide for a great introduction...

  35. We’re Going Viral This Week!

    Type
    Blog Post
    ... first paper came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to ...

  36. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...Ahem, ahem, drum roll please! It's time to announce the winners of our 2021 Deck the Lab Contest! Though...

  37. The Twelve Days of CRISPR

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    Blog Post
    ...the twelfth day of CRISPR, Addgene gave to me: multiplexed gRNAs.  We hope you’ve been enjoying our CRISPR...

  47. Countdown to Halloween @Addgene

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    Blog Post
    ... plastic and you have to share 1 trophy among 6 people, but you’ve earned bragging rights for the next...

  50. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...community is your negative data. We want to make sure people are selecting AAVs that will work for their experiments...
Showing: 851 - 900 of 1018 results