We narrowed to 964 results for: app

1. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   ...population, it’s easier to visualize individual/non-overlapping cells. Until now, tools for doing this were labor-intensive...

2. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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   ... understand or individual words that are not appropriate for the given context. After this first read,...

3. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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   ...PMC3713494.  Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal circuitry with an index-optimized clearing...

4. Fluorescent Biosensors for Measuring Autophagic Flux

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   ...on counting the number of autophagosomes. This approach, however, has inherent limitations because it ...

5. Multifunctional Neural Fibers: Modulation from Gut to Brain

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   ...interfaces with the entire body, so the obvious next applications are to expand the tool to different brain-organ...

6. Antibodies 101: Chimeric Antibodies

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   ...published, knockout validated, and work in your application. But…they are the same isotype and therefore ...

7. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   ...cells, and has potential use for a wide range of applications in cellular biology. Van Haren J et al. Nature...

8. Using Addgene's New Viral Service for Your Research

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   ...preparations have worked well in my lab, including in applications where we have delivered them in vivo to the ...

9. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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   ...a handle on some of these mysteries. Another application is clinical; can we find ways to treat patients...

10. Plasmids 101: Gram Negative and Positive Bacteria

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    ... added, it dehydrates the peptidoglycan layer, trapping the large dye complex created by the interaction...

11. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    ...you choose could be based on your downstream application, time, and instrument availability. UV absorbance...

12. New and Upcoming Viral Vectors - June 2019

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    ...ligand-gated ion channels that can be activated with FDA-approved drugs. See our inventory of DREADDs / Chemogenetics...

13. Visualizing Genomic Loci with CRISPR-Sirius

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    ...has been widely adapted for a wide variety of applications: base editing, CRISPR activation and inhibition...

14. Viral Vectors 101: Inverted Terminal Repeats

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    ... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and...

15. Creating accessible biology activities in schools with BioBits

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    ...mix chemicals together in chemistry to see what happens, but there isn’t really an equivalent of a “chemistry...

16. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    ...by TUNEL staining.  The video below shows what happens to the zebrafish’s heartbeat and blood circulation...

17. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    ...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...

18. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    ... an upright position for 30 seconds, and then recapping the tubes prior to thaw. We compared vented and...

19. The Power Behind NGS Plasmid Validation: seqWell

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    ... we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation Technology for...

20. Cultivating Community Science at BosLab

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    ...the biotech revolution. Our lab exists in what appears to be a funky old garage, but looks can be deceiving...

21. With Our New Viral Service, We're Taking CRISPR Further!

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    ...Numbers in the large colored circles are rough approximations of the total number of CRISPR plasmids for ...

22. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    ...The LipoGlo system represents a new, intriguing approach to expand our understanding of ApoB-LPs and accelerate...

23. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...

24. Building and Validating Recombinant Antibodies: A Case Study

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    ...antibodies that are specific to each subclass. Approximately 70% of mouse mAbs are IgG1 subclass (Manning...

25. Antibodies 101: Buffers, Storage, and Conjugates

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    ... to be stored either in a dark, opaque vial or wrapped in aluminum foil to prevent ambient light from ...

26. A Quick Guide to a Career in Software Product Management

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    ...realize that the bench isn’t where they will be happiest. What is software product management, and how ...

27. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    ...other ways to choose restriction enzymes for this approach. If you’re planning to go this route, check out...

28. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    ...Scientists can also use electroporation, the application of an electrical charge to cells, to increase...

29. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...

30. 15 Years of Addgene: The Top 15 Plasmids

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    ...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...

31. Early Career Researcher Toolbox: Social Media for Scientists

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    ...s advice to tap into the online conversations happening around a scientific conference, whether you are...

32. OCaMP: A New Calcium Indicator for Neural Imaging

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    ...Tool: The iGluSnFR3 Glutamate Sensor A Practical Approach to Choosing the B(right)est Fluorescent Protein...

33. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    ...discussed in this post, voltage has been adjusted appropriately and compensation performed. Before you read ...

34. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...

35. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    ... markers make it easy to spot flies with the appropriate transgene. One such marker is the white gene,...

36. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    ...interactions, cancer immunotherapy, as well as new approaches to reprogramming tumor-associated inflammation...

37. Improved Plasmid Maps Powered by SnapGene

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    ...can be detected is 5x greater than our previous mapping software and now includes Type IIS restriction ...

38. Screening for Successful Genome Editing with Digital PCR

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    ...a gene of interest is one of the most common applications of precision genome editing, the remainder of...

39. Top Requested Lentivirus and AAV of 2016

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    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...

40. How to Make Friends and Meet People at a Scientific Conference

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    ...relationships that are memorable. It isn’t always appropriate to walk up to someone much more senior than you...

41. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    ...take place in a tissue culture dish but can also happen in real-time inside a mouse’s brain.  But the brain...

42. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    ...the skin making it important to study what is happening within the keratinocytes. “We hope to use CRISPR...

43. RNA Extraction Without A Kit

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    ...potentially complicate results of a downstream application such as quantitative PCR to assess gene expression...

44. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    ... cells to the microenvironment. This mechanism happens for both short and long distances. When it comes...

45. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    ... assays Long-lived protein degradation: This approach measures the degradation rate of radio-labeled...

46. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    ...densely populated areas. This assumption does not appear to hold. There are institutions in Boston - Massachusetts...

47. Addgene's Tips for Plasmid Quality Control

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    ... glycerol stocks, so make sure to select the appropriate service and read the submission instructions....

48. The Future of Research Symposium Boston 2015

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    ...BEST) awards, which are designed to support new approaches that address the need to train our young scientists...

49. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ...there’s a lot of control spatially as to what’s happening at different points within the wood. There are...

50. Career Coaching for Scientists: Why and Where Do I Find One?

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    ...Industry Professionals”. This larger shop might appeal to some although most scientist coaches can help...