Progress Towards a PAM-Free CRISPR
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References
Kwart, D., Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing...
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Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...//doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...tropism of Nanoblades, different viral envelope protein(s) are expressed to pseudotype Nanoblades. The team ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post... http://abt.ucpress.edu/content/79/3/208 2. Fire, S. Xu, M.K. Montgomery, S.A. Kostas, S.E. Driver, C.... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...and other required enzymes, including a glutathione S-transferase homolog from a Janthinobacterium sp. Moreover... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...3308701. Find the BioID Plasmids Here! Macneill, S., & Varnaitė, R. (2016). Meet the neighbors: Mapping... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...interactive images of their proteins. Since the 1980’s, journal articles have been filled with impressive... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...in drug discovery. References 1. Baird, Geoffrey S., et al. "Circular permutation and receptor insertion... -
Tips for Technical Support Calls
TypeBlog Post... also let us know! Sometimes those few “thank you”s are what get a customer service agent through a busy... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...Pubmed Central PMCID: PMC4866822. 3. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...science.aaa5945. PubMed. DiCarlo, J. E., Chavez, A., Dietz, S. L., Esvelt, K. M. & Church, G. M. RNA-guided gene... -
15 Hot Plasmids from 2017
TypeBlog Post...their useful reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671 ...the popular type II CRISPR systems, which include S. pyogenes (Spy) Cas9, been identified. In Rauch, et... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...following four questions to figure out which method(s) work best for you, your audience, and your science... -
Pooled Library Amplifications
TypeBlog Post...and how many cells to use for your transformation(s). The amount of competent cells you need total and... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...References and resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...in several bacteria associated with human disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa)... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...PubMed Central PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal number of HIV genes required ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...broadly one can interpret our analysis of COVID-19’s effect on scientific activity. The effects of COVID...