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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 838 results for: ANG

Showing: 341 - 360 of 838 results

  1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...able to make the edits without any reagent-grade endangered Martian chicory root, but considering this is...you can better plan your transductions and use a range of reasonable MOIs. 2. Create a monoclonal Cas9-...lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described in Improved vectors and genome-...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038...

  2. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... Strebinger, D., Frangieh, C. J., Friedrich, M. J., Faure, G., Macrae, R. K., & Zhang, F. (2023). Cell...takes is a bit of chemistry magic to produce a wide range of useful molecules linked to that substrate. If... magnetic beads. In one innovative example, the Zhang lab used SNAP-tag to decorate synthetic viral particles...

  4. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...emission. The visible color spectrum is located in a range of wavelengths of about 380–700 nm. Photons of lower...electrons of a fluorophore, they can do so at a range of wavelengths, rather than at just one defined ...Likewise, the emitted photons also appear within a range of wavelengths. For that reason, we speak of excitation...conjugated to Cy7, however, (APC-Cy7) the brightness changes to "dim" for the same laser type. Knowing that ...

  5. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ... genes in mammalian cells (Bressan et al. 2017; Liang et al. 2017). Current methods of epitope tagging...Characterize clonal lines by ICC and PCR genotyping (Sanger sequencing); use primers flanking the tag sequence...References 1. Bressan RB, Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant ...Rev Genet 32: 601-618. PubMed PMID: 9928493. 4. Liang X, Potter J, Kumar S, Ravinder N, Chesnut JD. 2017...

  6. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...Although white light is entering your hand, only orange/red light can be seen exiting through the tissue... Light is projected through the coverslip at an angle such that when it reaches the interface between ...when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation and...confocal microscopy, 3rd edn. Springer (2006). 7. Yang, Weijian, and Rafael Yuste. In vivo imaging of neural...

  7. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and Chari/Mali... 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries for...: 27760321. PubMed Central PMCID: PMC5081405. 6. Wang, Tim, et al. "Gene essentiality profiling reveals...

  8. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... is working for you, then there is no reason to change it. But what if a kit isn’t working for you, or...kits are designed to accommodate specific volume ranges of culture as their input (Table 1). When you feel...addition to standard purification kits, there’s a range of specialty kits available for plasmid purification...you do want to make sure you’re getting the best bang for your buck. If you’re unsure if a kit is a good...

  9. Deck the Lab 2022!

    Type
    Blog Post
    ...by 草方 (@gracie_fang) That’s right, researchers of the world. Cap your Eppendorf tubes, hang up your ...at like 23:00, so I decided to make a giant snow angel for biologists in our building. Unfortunately, snow...

  10. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...the make-up and scope of your graduate projects changed throughout grad school? 22:05 - 26:10 : What's ... studies developmental gene regulation with Dr. Angela DePace in the Department of Systems Biology, and... genetics at the University of California, Los Angeles. She enjoys chromosome biology and reading Ben ...

  11. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...Host-parasite Interactions, Institut Pasteur, Paris) that changed genome editing in the malaria community for the...it all settles down and the value of each item changes from what the reviewers so painstakingly assessed...offline tools requiring expertise in one programming language or another. Supported organisms: This section...Top tool for first timers: You need to learn the language and what the parameters represent. The best learn-by-doing...

  12. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute and was designed and...take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR v2 has only been available... 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries for...

  13. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ..., in the labs of Chase L. Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing... author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future, it could...Powerful New Way to Edit DNA" and "This Protein Could Change Biotech Forever."  David S. Weiss of Emory University...

  14. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...find oneself unsure of the next steps. Harvard PI Angela DePace has put personal development at the center... is to give honest, constructive feedback, set tangible research and career goals, and celebrate accomplishments...getting used to, yearly planning meetings have tangible benefits for both PI and trainee. DePace and her...

  15. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project, if ...lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These programs can be...necessary time and resource investment required to change the status quo. But with recent improvements to...

  16. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...States - Cambridge, MA to be exact. Today, that changes. Today, Addgene launches a brand new Europe office...specialising in Molecular Biology. She has worked in a range of labs - cancer research, microbiology, virology...scientists together from all over the world to exchange research materials in an easy way. She has a passion...

  17. An Addgene Summer Internship

    Type
    Blog Post
    ...wanted to major in biochemistry. There, I had exchanged contact with Amy C., Addgene’s intern coordinator...into perspective just how much one’s environment changes them. Addgene gave me the chance to become a real...opened its arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want it ...

  18. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ... origin of replication (which controls the host range and copy number of the plasmid) and typically include...biologists to broadly utilize plasmids across a wide range of applications. Some common plasmid types include...Produce enzymes that will make specific, controlled changes to an organism’s genome (genome engineering) Produce...

  19. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...cytosine nucleotides, we might need to send it for Sanger sequencing to verify the sequence within that region... region. We have designed many primers for Sanger sequencing at Addgene. By using Snapgene’s “Import primers... difficult regions and send the plasmid off for Sanger sequencing.     If a repetitive region is present...

  20. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...). G. stearothermophilus grows at temperatures ranging from 30-74 °C, with an optimal growth temperature...or G) Spacer Length 20 nt 22 nt Temperature Range for Optimal Activity 35 - 45 °C 50 - 70 °C Genome...were incubated in reaction buffer at temperatures ranging from 25-80 °C. Activity is expressed as Kcleave...
Showing: 341 - 360 of 838 results