...biomedical research". The family entered the biomedical field’s spotlight after researchers discovered...animals have impacted humans through biomedical research. The Camelid family is composed of camels, llamas... llamas and alpacas (Figure 1). At the time, researchers were already on a quest for smaller forms of ...experiments, due to their simple structure    sdAbs in research These advantages have contributed to sdAbs becoming...variable domains (aka nanomice), which gives researchers the “best of both worlds” - a familiar lab model...

...Plasmids from a variety of research labs and hopes that these will help researchers build their knowledge ...efficiently in humans. This is why we cannot stop searching for novel solutions to treat rare diseases like...causes, and potential cures. We encourage any researchers working on ACVR1 or any of the pathways that ...about Addgene's Previous Work with Rare Disease Researchers Check Out Potentiall HIV Treatments with CRIPSR... Find ACVR1 Plasmids Find Plasmids for Disease Research on OurSpecial Collections Page ...

...Arabidopsis research and teaching community through the TRAINED initiative (Translating Research on Arabidopsis...Ohio, ABRC ships more than 100,000 samples to researchers and educators in 60 countries each year. Introducing...Arabidopsis Arabidopsis is an important model system for research and is used by scientists around the globe. As...Educational Resources). Arabidopsis is used in research to explore a variety of phenomena, and so too ...Park Conservatory. Do you use Arabidopsis in your research? Are you a biology teacher or professor? Whether...

...job specifically looked for a researcher with 3 years+ of cancer research experience, a PhD or a Master...what leaving academia feels like for a lot of researchers. For many years I struggled with the questions...lie ahead of me? Like a considerable number of researchers, I had high hopes of securing tenure in a British...time in the lab, my colleagues and what I was researching, but I decided I needed a new challenge. Looking...widely available, especially for life science researchers. Many biotechnology companies are looking for...

...modular approach made ZFNs highly appealing to researchers.  TALENs In 2010, transcription activator-like...at introducing point mutations. In 2016, the researchers at the Broad Institute envisioned a new strategy...consider the ethics and broader implications of our research.  Want the Genome Editing Trading Card seen in...understanding of the base editing enzymes. Kartik’s research focuses on the use of molecular dynamics simulations...our CRISPR guide Find CRISPR plasmids for your research Browse plasmids for base editing References Anzalone...PJ, Wilson C, Newby GA, Raguram A, Liu DR (2019) Search-and-replace genome editing without double-strand...specificity of a homing endonuclease. Nucleic Acids Research 30:3870–3879 . https://doi.org/10.1093/nar/gkf495...

...fragments that are then barcoded for identification. This mixture of barcoded fragments is known as a ...sequencing of the fragments within a library allows researchers to reconstruct the full individual sequence. ...large number of samples are subjected to a DNA-barcoding step that uniquely labels DNA from each well of... containing fragments carrying sample-specific barcodes. Rather than continuing to process 100s to 1000s... of input samples. To minimize these issues, researchers usually include a separate normalization step...

...Eckhardt and Melanie Brewer from the QBI Coronavirus Research Group at UCSF. It’s been only a few months since... more than 20 labs to form the QBI Coronavirus Research Group (QCRG) to do just this.  In under a month...with requests to share our plasmids with other researchers. We have been excited to see so much interest...problem more quickly. Figure 2: Getting our research in line, with a row of FedEx envelopes waiting...shared spreadsheet and it takes time away from our research. For all these reasons, the QCRG is delighted ...requesting plasmids for coronavirus or COVID-19 research, please email Addgene at [email protected] with...

... deal of scientific research is funded by the public and performed by researchers who received their educations...even minor weakness or limitations in a piece of research (Drummond & Fischhoff, 2017; Kahan et al., 2012...However, much of the information gained from that research remains inaccessible, behind paywalls and in technical...the needs of the people we are talking to. Emma Harcourt is a PhD student in the Department of Science ... Communication at the University of Otago. Her research focuses on misinformation about sexual and reproductive...

...scientists will choose to start their own academic research laboratory. How does one even go about starting... But how does one build a strong collaborative research team motivated by similar visions and goals? We...the lab. “An effective working environment for research is totally dependent on communication amongst ...for some quick advice and resources for hiring research staff. And for a more indepth resource for starting...departmental administrator, lab and safety manager, researcher, adviser, conference planner, and writer.” Connie...: Running Your Lab (pdf) Advice for Choosing a Research Project 9 Tips to Achieve Success in Academia  ...

...needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully complete...GCE provides almost endless possibilities for researchers, from selective protein ligation through introducing...literature into robust and universal techniques that researchers can easily implement in their own labs. They ...working to make GCE available for all biomedical researchers. If you’re interested in using GCE in your experiments...protocols would be broadly useful for biomedical researchers. One way of doing that is by using Addgene’s ...would be useful. Think GCE could be useful in your research? We definitely recommend checking out GCE4All’...

... money to support this research. But there is a move towards more open research. The EU, for example, ...community to create state-of-the-art devices for research. There are a number of new platforms (such as .... Direct sharing of work from universities and research centers provides a potential conflict between ...can lead to faster and more efficient science. Researchers around the world don't have to start from scratch...“openUC2” and focuses on bringing cutting-edge research to everybody by relying on tailored image processing...

...adapted CRISPR nucleases for many more specific research needs. In this update, we will focus on the most... variants may be appropriate for your specific research question. Considerations when choosing your Cas...recognize a variety of different PAM sequences. Researchers have also used experimental evolution to develop...that may make it especially suitable for in vivo research and potentially treatments. Cas12b, characterized...al., 2018) Labs also shows promise for in vivo research due to its small size and specificity relative..., it is likely that the toolkit available to researchers will continue to expand. Final thoughts Since...

...provide a much needed boost to basic molecular plant research, more initiatives are needed to increase the body...Organizations like Open Plant can then build on this research and effectively carry out their mission of creating...selected a project in a field with 30 years of basic research standing behind it. Our color changing flower ... Petunias have been a model organism for plant research for decades, and the biochemical pathways involved...circadian rhythm in petunias. Because of the extensive research that has come before us, it’s possible for us ... flowers that change color. Without this basic research – this comprehensive understanding of the genetic...

...environments has never been easy and, for many years, researchers lacked good tools to directly follow immune cells...thymus to the lymph nodes. These methods gave researchers much more information regarding immune cell mobility...coloured lymphoid organs did in the past. How do researchers track fluorescently labeled cells? 1. Epifluorescence...For the first time, fluorescent proteins gave researchers the ability to track immune cells within organs... the next revolution in cell imaging, giving researchers the ability to monitor cellular movements in ...cells is the the Cre/lox recombination system. A researcher can flank fluorescent proteins in a plasmid with... PubMed Central PMCID: PMC3164316. 5. Chieppa, Marcello, et al. "Dynamic imaging of dendritic cell extension...

...technology that’s already had a huge impact on research and will be affecting our everyday lives very ...visualizing genomic loci, and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved...effects, respectively. Recognizing these issues, researchers from the Zhang lab at the Broad Institute, the...structure leads to the development of HypaCas9 Researchers in the Doudna Lab at Berkeley recently added ...eSpCas9, SpCas9-HF1, and HypaCas9 should enable researchers to make precise edits in mammalian cells and ...question very soon! We look forward to seeing how the research community makes use of these tools and are excited...

... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...recombinant viruses typically used in neuroscience research (e.g., AAV) are useful for labeling the individual...recombinant forms of these viruses typically used in research aren’t able to replicate, and for a virus to cross...receptor (TVA) in user-defined cells. For example, a researcher can deliver Cre-dependent TVA to Cre-expressing... (i.e., it is taken up by projection neurons). Research into these phenomenon has shown that the RABV ...

...team effort and many late nights, not unlike most research, but the urgent need for the test did catalyze...yet never hesitated to reach out to the greater research community when we needed additional expertise....uses portions of the pathogen’s genome as unique barcodes to identify samples containing the virus. Of the...rapid sharing of these tools with the Zika virus research community. Find the Zika Sensor Constructs at ... of Pharmacy at the University of Toronto. His research is focused on developing cell-free synthetic biology...Arizona State University. His group carries out research in synthetic biology, nanomaterials science, and...

...molecular biology in our Plasmids 101 eBook! Researchers use various techniques to control protein expression..., biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them... also be purified for use in molecular biology research (eg. polymerases and other enzymes might be purified...types of plants such as maize, tobacco, rice, sugarcane, and even tubers of potatoes have been used for...and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins under the control...Resources on Addgene.org Bacterial expression systems Search for plasmids by expression system ...

... more than 10 modules often rely on two-step hierarchical approaches using different Type IIS restriction...Development of Golden Gate Assembly test systems Our research focused on three different levels of assembly:...number of transformants. To compensate for this, researchers can plate larger volumes of the outgrowth containing... to minimize this possibility. Recently, NEB researchers profiled the comprehensive fidelity of cohesive...important tools for many areas of science, and researchers continue to test the limits of DNA assembly approaches...and bias during DNA end-joining." Nucleic acids research (2018). PubMed PMID: 29741723. PubMed Central ...

...Principle Investigators (PIs) - whose labs does research that aligns with your interests and goals.  Reaching...gives a more comprehensive idea of that advisor’s research interests which can be helpful in creating your...where you can further discuss aspects of the lab, research, and provide them with a CV or transcripts if ...interests, but will ask you questions about your research goals and education, too! Prepping for the interview...quickly reference your questions about the PI’s research or the name of the award you won for your conference... student at George Washington University whose research focuses on Drosophila-parasite pathogen interactions...