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  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer vector...
  2. Plasmids 101: Using Transposons in the Lab

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    Blog Post
    ...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...
  3. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...
  4. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...absorbed by matter, they promote electrons within atoms to a higher energy state. This process is called.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...
  5. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... unbiased discovery. With these screens, the investigator generates a cell population where all genes ...contexts. Most of the immune cells we study are migratory, and their exact localization is important for...
  6. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post
    ...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...and purification is a snap using thin layer chromatography (Fessenden, 2009). However, these reagents ...
  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... electrophysiology data too but that’s what collaborators are for). For example, if you study neural pathways...projection-specific neuronal populations using combinatorial viral approaches. Current protocols in neuroscience...
  8. Using AAV for Neuronal Tracing

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    Blog Post
    ...essential to build up our current understanding of neuroanatomy and neural connectivity, providing valuable ...conditional transgenic mice, to reveal not only the anatomy of neuronal connections, but to visualize neuron...
  9. Zinc Finger Consortium Reagents

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    Collection
    ...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional...
  10. KLF Research Plasmids

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    Collection
    ...family of at least 17 DNA binding transcriptional regulators with diverse roles in processes such as cell ...
  11. Fluorescent Protein Guide

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    Collection
    ...signals, cells, or groups of cells with either actuators or sensors. Chemogenetics Plasmids | Guide Use...
  12. AAV Viral Preps

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    Collection
    ...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic...
  13. CRISPR Plasmids and Resources

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    Collection
    ...Transcriptional Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9...
  14. FlyCRISPR

    Type
    Collection
    ...designed for in vivo transcrition controlled by the regulatory sequences of an RNA polymerase III transcribed...
Showing: 341 - 360 of 641 results