We narrowed to 641 results for: ATO

...available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first...Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389...

...carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal...

...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...own replication machinery such as Rep or other initiator proteins, they do not require host proteins for...

...signal inside the tumors was overlaid with the anatomical B-mode images in greyscale.   ARG expression...-specific ultrasound signal is in hot and the anatomical B-mode images are in greyscale. (scalebar = 2mm...

...versatility and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this....    References 1. Stuber, Garret D., et al. "Excitatory transmission from the amygdala to nucleus accumbens...

...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...

...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency...line and ~8 and 9% efficiencies in T-cells and hematopoietic stem cells respectively. Future researchers ...

...know is your area code. Senators: To find contact information for your senators all you need to do is select...

...Livet, Jean, et al. "Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous...liver progenitors in mouse oval cell injury." Hepatology 60.1 (2014): 278-289. PubMed PMID: 24700457. ...

...phosphorylation sites. If knowledge of a candidate regulatory kinase is known, it’s possible to generate non-phosphorylated...Often, however, researchers lack knowledge of the regulatory kinase, access to active kinase for the reaction...

...turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system...W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust ...

...academic publishing goals and make you a better communicator overall. Don’t Write in a Vacuum Your research...through many iterations read by your PI, your collaborators, others you know in the field, and even people...

...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...

...nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization ...RNA-seq would be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would...

...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...

...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...

...reliably detect single-base differences. The original creators of this technique visualized the ligation product... the Thermostable Ligase Reaction Temperature Calculator can be used to estimate an appropriate incubation...

...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer vector...

...group focuses on gene regulation in nematode molting and spermatogenesis. His group also builds tools to ...

... unbiased discovery. With these screens, the investigator generates a cell population where all genes ...contexts. Most of the immune cells we study are migratory, and their exact localization is important for...