...that covers most things CRISPR - from nickases to base editing to pooled library screening. It also has...

...Open Hardware? Author: Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author...AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers...YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic ...

...the PCR is complete, the product can be verified based on size by gel electrophoresis . Protocol Video ...hydrogen bonds are broken between the nucleotide base pairs. The initial denaturation step is commonly...temperature (Tm). Set extension step at 1-2 minutes per kilobase of product depending on whether you are using ...

...causes of irreproducibility with some solutions based on what I’ve learned. I hope you can apply some ...all human extracellular proteins with community-based validation. They also have plans to expand to other...with larger and larger datasets and use computer based analysis techniques, an important component of reproducibility...on the Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific...technologies later. Organizations such as BenchSci and FPbase are working hard to enable researchers by making...BenchSci collates information about antibodies while FPbase curates information about fluorescent proteins ...

...TAG. Design your repair template with 55-100 base homology arms upstream and downstream of the insertion...using the ivTRT method, try to make the first two bases of the left homology arm guanine (i.e. GG); this... PMC3961003  Miura, Hiromi, et al. “CRISPR/Cas9-based generation of knockdown mice by intronic insertion...

...References Abril, U.N.O., et. al. (2022) A plasmid-based system for locating and containing escaped flies...

...the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in ...

... delivery systems, especially lentiviral vector-based CRISPR pooled libraries that allow functional screening...barcoding library consists of 96 retroviral plasmids (based on the Murine Stem Cell Virus, MSCV) each with a...

... broadens the conversation about genetics pgEd, based at the Department of Genetics of Harvard Medical...universities or cities have organizations (often volunteer-based) that put on public lectures, science cafés or science...

...protein for live cell imaging would be mTurquoise2, based on the data acquired at low excitation powers. Note...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene...

... on the identification of autophagic structures based on morphology. Autophagosomes are relatively easy...summary of what color fluorescence Rosella emits based on its localization. Table 1: Summary of Rosella...

...and sensitive enough to detect gene copy number based on the number of fluorescent puncta observed. By...Addgene Blog Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent...

...the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors to monitor intracellular signaling...

...the concentration of proteins in an ELISA well is based on a colorimetric reaction. Most commonly, this ...concentration of protein in the experimental wells based on the equation of the line. With these tips in ...

... modified) for optimal stability and efficacy.  Base modifications What is more stable than RNA? DNA!...gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https...

...track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a new tool developed... quantification of this depletion using imaging-based measurements (Ashley et al., 2021).   Schematic...

...learn how to communicate their work differently based on their audience. How you talk about your experiments...problem was the creation of an Addgene lexicon. The base lexicon was and continues to be a list of commonly...

...barcoded gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone...: PMC4420636. 7. Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-...

...supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz Lab Bacterial...Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window) Return to top...

...with CRISPR/Cas9 in Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen...WormAtlas - A database featuring behavioral and structural anatomoy of C. elegans. WormBase - A consortium...