We narrowed to 382 results for: Bre;

1. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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   ...-1  Pennacchio, L. A., Bickmore, W., Dean, A., Nobrega, M. A., & Bejerano, G. (2013). Enhancers: five ...

2. CRISPR 101: Any Base Transversion Editors

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   ...also called apurinic or apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David...

3. Hot Plasmids: Fall 2024

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   .... abscessus. A) Sth1Cas9 mediates double strand break at the target site, which results in either bacterial...

4. Plasmids 101: Stringent Regulation of Replication

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   ...strands. Created with BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together...

5. Antibodies 101: Reading a Flow Plot

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   ...first… which can send you in an endless loop. Let’s break out of the loop with all you need to know on how...

6. Viral Vectors 101: Preparing Pooled Libraries

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   ...incubation time, antibiotic conditions, and amount of polybrene. (psst…didn’t get your library pre-made from Addgene...

7. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ...engineering single genome edits but the double strand breaks it introduces makes it difficult to perform two...

8. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ... Cpf1), a nuclease that generates double strand breaks in target genes resulting in the formation of “...

9. Finding nucleic acids with SHERLOCK and DETECTR

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   ... generates 5’ and 3’ staggered ends after dsDNA breaks. The Doudna lab discovered Cas12a’s ability to ...

10. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...matching Mentors with Singletons for short coffee breaks. While this was not the more intense mentorship...

11. An Inside Look into Addgene's Dev Team

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    ...collaborative approach to development. Scrum teams plan and break up work into small, well defined chunks, called ...

12. The Challenges of Cell Culture

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    ...2002): 315-319. PubMed PMID: 12001993. 4. Lucey, Brendan P., Walter A. Nelson-Rees, and Grover M. Hutchins...

13. Getting the Most from Your Lentiviral Transduction

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    ...typically employ the positively charged polycation, polybrene. Interestingly, one study found that alternative...

14. Important Considerations in Optogenetics Behavioral Experiments

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    ...behavioral effect but the function of specific subregions within the hippocampus were only recently studied...

15. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ...is very efficient at introducing double stranded breaks (DSBs) into genomic DNA in many cell types and ...

16. March for Science

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    ...cuts (3, 12, 13, 27). In order to achieve the breakthroughs, technologies, and treatments promised by modern...

17. Plasmids 101: Expressing shRNAs for RNA Interference

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    ...transfection or viral delivery of a plasmid. RNAi is an umbrella term for a number of different applications that...

18. Antibodies 101: Introduction to Immunofluorescence

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    ...and CiteAb. The antibody you choose can make or break your experiment, so take advantage of all the available...

19. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    ...South Korea, one of the earlier epicenters of the outbreak ultimately flattening the curve, it has become...

20. Evolution of Lab Techniques

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    ...chain-terminating or dideoxy sequencing, was a major breakthrough for determining the order of nucleotides in ...