We narrowed to 802 results for: Clu

1. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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   Blog Post

   ...colleagues built a Molecular Expressions collection including some 3,300 plasmids along with image galleries...specific cellular structures.The Davidson lab even included some of their own reference images that can be...

2. New Viral Vectors - Fall 2024

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   Blog Post

   ...available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the AAV9-X1.1...have a bunch of other new AAV vectors as well — including next-generation biosensors for GABA, dopamine,...

3. Antibodies 101: Affinity Tags

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   Blog Post

   ...tags, often called polyhistidine tags, typically include six consecutive His residues (or more). Histidine... tags There are many different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent...

4. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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   Blog Post

   ... A free, donation-based plasmid analysis tool including editing, annotating, creating maps, and more. ...  Expasy: Bioinformatics resource portal which includes both bioinformatics tools and knowledge databases...

5. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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   Blog Post

   ...Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles...have hollow interiors and a protein shell that excludes water but is gas permeable. These vesicles are...

6. Fluorescent Proteins 101: Fluorescent Biosensors

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   ...biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin... (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium sensor...

7. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   Blog Post

   ...affected. Previous tools to overcome this restraint include an AAV-based sparse labeling system where a limiting...recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable ...

8. Understanding GPCR Signaling Using TRUPATH

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   Blog Post

   ...receptors that play many roles in signaling processes including smell, taste, sight, inflammation, and neurotransmission... could be measured using the TRUPATH system. Conclusions Although the importance of GPCR pathways is well...

9. Running for Rare Disease, Running for FOP, Running for AJ

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   Blog Post

   ...members of the ubiquitous activin ligand family, including Activin A. A commercially developed therapeutic...analytical development. His areas of expertise include early stage drug discovery and pre-clinical development...

10. Management for Scientists: What Makes a Good Manager Anyway?

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    Blog Post

    ...appreciated and valued This often stems from being included in organizational decision-making. It is imperative...things, diversity of responsibilities, which might include training or teaching others, research, and policy...

11. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post

    ...technological biological blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas...in E. coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against...

12. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    Blog Post

    ...and is based on the pTRC99a plasmid. It does not include the PBAD promoter. If you already have a pBAD33...the plasmids deposited from Di Ventura's lab, including the popular light-inducible nuclear export system...

13. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    Blog Post

    ...Would you buy and use a plasmid if it didn’t include any plasmid sequence data? Of course not. Unfortunately... plasmid-based and offer numerous advantages, including more secure archiving, unambiguous molecular definition...

14. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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    Blog Post

    ...but suffer from several important shortcomings including low processivity (ie. the probability of the polymerase...transport from the cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP ...

15. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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    Blog Post

    ...module. As a general guideline, experiments that include growing plants require pots, trays, soil, fertilizer...efforts. Our education partnerships have grown to include local school districts, professional education ...

16. CRISPR Challenges: Standardization and Homology Directed Repair

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    Blog Post

    ...standardized methods for evaluating CRISPR technologies, including the ever important on/off-target efficiency. Likewise...guest bloggers Dominik Paquet and Dylan Kwart, including a detailed look at how cut-to-mutation distance...

17. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    Blog Post

    ...control Historically, AAV vector QC has been sparse including only titration of AAV particles and a protein ... the expression cassette that we want, it also includes a small amount of spurious DNA from the packaging...

18. Plasmids 101: Origin of Replication

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    Blog Post

    ...found in bacteria. Some common ones you might see include ColE1, pMB1 (which comes in a few slightly different...questions to get started: Will the plasmid be used exclusively in E. coli? Gram negative bacteria in general...

19. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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    Blog Post

    ...association, started a speaker series or biotech club, managed their own blog or podcast, initiated and...future and the 1:1 format is good for everyone including those that find events stressful. Video chatting...

20. Summer SciComm Series: Master of SciComm

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    Blog Post

    ... to do the research itself. Several years of exclusively writing lab reports and empirical essays had ...of those entering science communication (myself included) come from a scientific background and bring with...