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  1. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...tags, often called polyhistidine tags, typically include six consecutive His residues (or more). Histidine... tags There are many different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent...

  4. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...receptors that play many roles in signaling processes including smell, taste, sight, inflammation, and neurotransmission... could be measured using the TRUPATH system. Conclusions Although the importance of GPCR pathways is well...

  5. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...members of the ubiquitous activin ligand family, including Activin A. A commercially developed therapeutic...analytical development. His areas of expertise include early stage drug discovery and pre-clinical development...

  7. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin... (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium sensor...

  8. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...appreciated and valued This often stems from being included in organizational decision-making. It is imperative...things, diversity of responsibilities, which might include training or teaching others, research, and policy...

  9. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    .... A quick search for "CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats...few references for the double nickase system, including one recently from the Zhang group. Learn more ...

  10. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...technological biological blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas...in E. coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against...

  11. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ... to do the research itself. Several years of exclusively writing lab reports and empirical essays had ...of those entering science communication (myself included) come from a scientific background and bring with...

  13. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...found in bacteria. Some common ones you might see include ColE1, pMB1 (which comes in a few slightly different...questions to get started: Will the plasmid be used exclusively in E. coli? Gram negative bacteria in general...

  20. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...number of unique features compared to Cas9. These include: More targeting flexibility Unlike Cas9 and the...base excision would alter the target sequence and preclude future HDR, which could lower HDR efficiency. ...
Showing: 341 - 360 of 793 results