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We narrowed to 768 results for: Dos

Showing: 341 - 360 of 768 results

  1. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...observe your cells on a flow plot, you might have to do some testing or ask experienced colleagues. ...warning message at the top, as our cells of interest do not lie beyond the dimensions displayed in the plot...4). As H and A scale in proportion (while H and W do not; see Figure 2), single cell events will show ...

  2. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...the chance of off-target effects, you still need to do your due diligence to ensure they are indeed a non-issue...off-targets don’t mess with your results when they do arise. Happy CRISPR’ing! References and Resources...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9...

  3. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...and vocabulary for this skill. What this post may do is help you realize whether or not you have a natural...t mean you have to like conflict. Very few people do, and it’s best to be wary of anyone who is actually...there is a potential for long-term gains when you can do it. If Party X proposes solution A, and Party Y proposes...

  4. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...increase therapeutic efficacy (Ahmed et al., 2012). How do you generate an scFv?  scFv sequences have traditionally...antibody genes from diseased or vaccinated humans. How do you produce scFvs? While it’s possible to produce...scFvs are even used to screen for new antibodies. To do this, antibody variable domain sequences are expressed...

  5. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... this given that the brain controls everything we do, but I was quite impressed by how neuroscientists...to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog Advanced...

  6. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...unbound) population. The benefit of beads is that they do not require cells from your experiment, and they ...prefers to use them to gate our populations, but others do not. Some journals require isotype controls, while...

  9. Antibodies 101: ChIP

    Type
    Blog Post
    ...process is very similar for both, though tissue samples do require an extra homogenization step. For this blog...courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick review. At this point you have (1) crosslinked...

  11. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...? Blue-white selection is a widely used method to do just that! (To read about other screening strategies...with this method to ensure that positive colonies do have the plasmid containing the lethal gene. An example...

  13. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...unexplored areas with our color-changing flower, but to do that we’ve had to navigate the challenges involved... plant synthetic biology. We cannot build what we do not understand, and the complicated and unique metabolic...

  14. The Twelve Days of Antibodies

    Type
    Blog Post
    ... to our blog and social media. This year, we're doing so through the twelve days of antibodies. We hope...

  15. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...since most universities and non-profit organizations do not have the April 1 H-1B queue process or quota ... the performing arts- not relevant at all. Please do not include the fact that you are a classically trained...self-petition. You need the support of your institute to do this one. Universities will usually only support ...who could be beneficial during a career search. Doing these simple things will make it easier for you ...

  19. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses...promoter is no longer present in these constructs. Why do many γ-retroviral plasmids have similar names? The...

  20. Clear and Accessible Writing

    Type
    Blog Post
    ...questions: What is the goal, who is my audience, and what do I want to be different for them when I’m done? What... Excellent! We asked our workshop participants to do the same thing, and even though only about 10% of...
Showing: 341 - 360 of 768 results