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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 772 results for: Dos

Showing: 341 - 360 of 772 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...ends results in the production of blue colonies that do not need to be picked and sequenced for potential...PCR, restriction digest, or sequencing. Pro tips Do not add 5’ phosphates to your PCR primers; you need...

  2. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ..., but from what was necessary in order for you to do your research. For example, did you help put together...extracurricular activities – did you volunteer to do science outreach and teach children about science...

  3. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...students and postdocs when starting out. She advises “do not hurry to staff up the lab and to take things ...of great projects as ‘impossible' or useless.” How do you run your lab differently or the same as your ...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...single-stranded DNA (ssDNA) genome. Non-enveloped viruses do not have a lipid bilayer surrounding the viral particle.... AAVs are therefore non-pathogenic and generally do not trigger a strong immune response. These features... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...vectors present low oncogenic potential because they do not integrate in the human chromosome, which reduces...

  5. 2022 Depositor Week

    Type
    Blog Post
    ... Specialized Depositor Shout-Out  We'll also be doing giveaways on Twitter, so keep an eye out there for...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... Cas9 orthologs also have distinct PAM sites that do not cross react, allowing multiple Cas9s to be used...have also developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors...

  8. Arf GTPase Family

    Type
    Collection
    ...opens in a new window) Last reviewed on: 18 Jun 2025 Do you have suggestions for other plasmids that should...

  9. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...observe your cells on a flow plot, you might have to do some testing or ask experienced colleagues. ...warning message at the top, as our cells of interest do not lie beyond the dimensions displayed in the plot...4). As H and A scale in proportion (while H and W do not; see Figure 2), single cell events will show ...

  10. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...the chance of off-target effects, you still need to do your due diligence to ensure they are indeed a non-issue...off-targets don’t mess with your results when they do arise. Happy CRISPR’ing! References and Resources...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9...

  11. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...and vocabulary for this skill. What this post may do is help you realize whether or not you have a natural...t mean you have to like conflict. Very few people do, and it’s best to be wary of anyone who is actually...there is a potential for long-term gains when you can do it. If Party X proposes solution A, and Party Y proposes...

  12. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...increase therapeutic efficacy (Ahmed et al., 2012). How do you generate an scFv?  scFv sequences have traditionally...antibody genes from diseased or vaccinated humans. How do you produce scFvs? While it’s possible to produce...scFvs are even used to screen for new antibodies. To do this, antibody variable domain sequences are expressed...

  13. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...unbound) population. The benefit of beads is that they do not require cells from your experiment, and they ...prefers to use them to gate our populations, but others do not. Some journals require isotype controls, while...

  14. Antibodies 101: ChIP

    Type
    Blog Post
    ...process is very similar for both, though tissue samples do require an extra homogenization step. For this blog...courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick review. At this point you have (1) crosslinked...

  16. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... this given that the brain controls everything we do, but I was quite impressed by how neuroscientists...to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog Advanced...

  19. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...? Blue-white selection is a widely used method to do just that! (To read about other screening strategies...with this method to ensure that positive colonies do have the plasmid containing the lethal gene. An example...
Showing: 341 - 360 of 772 results