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...increase therapeutic efficacy (Ahmed et al., 2012). How do you generate an scFv?  scFv sequences have traditionally...antibody genes from diseased or vaccinated humans. How do you produce scFvs? While it’s possible to produce...scFvs are even used to screen for new antibodies. To do this, antibody variable domain sequences are expressed...

...the chance of off-target effects, you still need to do your due diligence to ensure they are indeed a non-issue...off-targets don’t mess with your results when they do arise. Happy CRISPR’ing! References and Resources...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9...

...observe your cells on a flow plot, you might have to do some testing or ask experienced colleagues. ...warning message at the top, as our cells of interest do not lie beyond the dimensions displayed in the plot...4). As H and A scale in proportion (while H and W do not; see Figure 2), single cell events will show ...

...we have found ourselves adapting to a new way of doing things. These new processes have been instrumental... what we’re here for and we’re glad to be able to do so during this critical time. Visit our COVID-19 ...

...similarity. REPLACR-mutagenesis: Letting bacteria do the work If you’ve conducted SDM using some of these...on in vitro assembly, they could make the E. coli do the work by using the principles of recombineering...

...process is very similar for both, though tissue samples do require an extra homogenization step. For this blog...courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick review. At this point you have (1) crosslinked...

...unbound) population. The benefit of beads is that they do not require cells from your experiment, and they ...prefers to use them to gate our populations, but others do not. Some journals require isotype controls, while...

... this given that the brain controls everything we do, but I was quite impressed by how neuroscientists...to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog Advanced...

...elute YFP, you turn on the blue lights. Which option do you choose?  Option #2 may sound like fiction, but...monomeric variable heavy chain antibody domain.  How do these components interact? When fused together to...

...? Blue-white selection is a widely used method to do just that! (To read about other screening strategies...with this method to ensure that positive colonies do have the plasmid containing the lethal gene. An example...

... all driven by personal connections What can you do to get the most from the time and money you are spending...about how you want to introduce yourself and what you do. You can probably use some scientific terms when ...

...unexplored areas with our color-changing flower, but to do that we’ve had to navigate the challenges involved... plant synthetic biology. We cannot build what we do not understand, and the complicated and unique metabolic...

...opens in a new window) Last reviewed on: 18 Jun 2025 Do you have suggestions for other plasmids that should...

...Technology Content last reviewed: 10 September 2025 Do you have suggestions for other plasmids that should...

... to our blog and social media. This year, we're doing so through the twelve days of antibodies. We hope...

... model a specific disease or process of interest. Do you want to: Generate complete and permanent loss...target cells are not transcribing DNA to mRNA, nor do they need to translate mRNA to protein. Instead, ...a lack of efficient target cleavage in cells that do express both Cas9 and gRNA. Before choosing a validation...using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA method described...

...since most universities and non-profit organizations do not have the April 1 H-1B queue process or quota ... the performing arts- not relevant at all. Please do not include the fact that you are a classically trained...self-petition. You need the support of your institute to do this one. Universities will usually only support ...who could be beneficial during a career search. Doing these simple things will make it easier for you ...

...areas of research. We welcome your comments... Where do you think these technologies are going? What is the...

...promoter region.   Ok, I have a Cas9! What do i do next? Designing or selecting a sgRNA Addgene may...

...your email for confirmation of submission. If you do not receive such a notice within 24 hrs, send a note...while you are climbing the academic ladder. If you do not get accepted to your top choice this year but...