We narrowed to 753 results for: ELP
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TypeBlog Post... probes, we decided to write this quick guide to help researchers choose the probe for their needs. You...assays with other FPs. We hope that this post helps you in getting to know the state-of-the-art NIR ...
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Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post... phage display library with plate-bound antigen helps select for high affinity scFvs. Bound phage are ...expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm (Sandomenico... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...muffled voices beneath masks, humans rely on mouths to help them hear and read emotional cues. We had two options...written and video protocols at Addgene? If you need help in the lab, we have you covered. ... -
Plasmids 101: Biotinylation
TypeBlog Post...biotinylation reagents to help with the specific elution of biotinylated proteins. For help determining which ... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...downstream of the 3’ splice acceptor of the transgene to help prevent the accumulation of such truncated proteins...understanding of basic AAV biology, however, would help improve split vector strategies and lead to greater... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post... your gel. At their most basic, loading controls help you rule out errors like uneven pipetting or irregular...necessary to demonstrate a POI’s absence. It also helps confirm that lanes are loaded evenly. Created with... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post... than 48℃. Both Snapgene and NEB have tools that help you design primers for PCR amplification of fragments...incorporate such regions of homology. This video gives a helpful demonstration of how to use Snapgene’s program ... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...publication, improving your general writing skills will help you in your academic publishing goals and make you...reach out to your peers and other mentors who can help you. You Can Talk to Your Editors I wish I had... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression... CRISPR has made targeting much easier! With the help of guide RNAs, fusions between Cas proteins and ... -
A Control for All Seasons
TypeBlog Post...world where so much is out of your hands, it’s helpful to focus on something controllable, like experiments...results of a single mouse. Biological replicates help you to determine which responses arise from drug... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...options to consider, and the following factors will help you determine which one(s) are candidates for your...are a number of optogenetic tools now available to help light your path! Resources and references Recommended... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...You’ll need to consider if such properties could help, or harm, your experimental goals when designing... other posts in our Viral Vectors 101 series can help guide you with these, so keep reading! Good luck... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...deposit your piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot...nucleases. Mathew applies his specialized knowledge to help researchers identify the right technologies and ... -
Antibodies 101: Validation
TypeBlog Post...likely won’t work in a given application is just as helpful as knowing when it will. And remember, antibodies...others in the community can benefit and we can all help improve antibody reproducibility! References... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...So, labeling a HaloTag or SNAP-tag construct can help you better detect dim structures or low levels of...are comfortable with it or can find a colleague to help you, synthesizing your own ligands can open up more... -
Quick Guide to All Things Lentivirus
TypeBlog Post...polybrene (protocol) or DEAE dextran (protocol), which help the viral particle bind to your target cells. If...packaging cell lines to avoid recombination leading to helper virus production." Molecular and Cellular Biology... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...they encapsulate, and the solutions they promote help motivate you to spend a little time advocating for...science in policy, demanding better funding, and helping others understand the value of science is an ongoing... -
Antibodies 101: Flow Cytometry
TypeBlog Post...If this sounds like you, then flow cytometry can help you overcome these challenges and make these types...except one. The fluorescence-minus one panel will help you set up gating during data analysis. We hope... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post... the many questions scientists have been able to help answer using two-photon microscopy include: How...technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better tools and models... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...what we discuss. If you can think it, MoClo might help you achieve it! MoClo applications and specialized...2017). If you can dream up an experiment, MoClo can help you achieve it! More recently, a CRISPR MoClo kit...