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We narrowed to 755 results for: ELP

Showing: 341 - 360 of 755 results
  1. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...are a few simple troubleshooting measures that can help get you back on track: Compatibility: Double-check...inducer or adding an affinity tag such as GST may help with solubility issues. We want to hear about your...
  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... probes, we decided to write this quick guide to help researchers choose the probe for their needs. You...assays with other FPs.   We hope that this post helps you in getting to know the state-of-the-art NIR ...
  3. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post
    ... phage display library with plate-bound antigen helps select for high affinity scFvs. Bound phage are ...expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm (Sandomenico...
  4. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...muffled voices beneath masks, humans rely on mouths to help them hear and read emotional cues. We had two options...written and video protocols at Addgene? If you need help in the lab, we have you covered.  ...
  5. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...biotinylation reagents to help with the specific elution of biotinylated proteins. For help determining which ...
  6. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...publication, improving your general writing skills will help you in your academic publishing goals and make you...reach out to your peers and other mentors who can help you. You Can Talk to Your Editors I wish I had...
  7. A Control for All Seasons

    Type
    Blog Post
    ...world where so much is out of your hands, it’s helpful to focus on something controllable, like experiments...results of a single mouse. Biological replicates help you to determine which responses arise from drug...
  8. Viral Vectors 101: Optogenetic Tools

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    Blog Post
    ...options to consider, and the following factors will help you determine which one(s) are candidates for your...are a number of optogenetic tools now available to help light your path! Resources and references Recommended...
  9. Viral Vectors 101: AAV Variables That Matter

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    Blog Post
    ...You’ll need to consider if such properties could help, or harm, your experimental goals when designing... other posts in our Viral Vectors 101 series can help guide you with these, so keep reading! Good luck...
  10. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post
    ...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression... CRISPR has made targeting much easier! With the help of guide RNAs, fusions between Cas proteins and ...
  11. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...deposit your piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot...nucleases. Mathew applies his specialized knowledge to help researchers identify the right technologies and ...
  12. Antibodies 101: Validation

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    Blog Post
    ...likely won’t work in a given application is just as helpful as knowing when it will. And remember, antibodies...others in the community can benefit and we can all help improve antibody reproducibility!     References...
  13. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...polybrene (protocol) or DEAE dextran (protocol), which help the viral particle bind to your target cells. If...packaging cell lines to avoid recombination leading to helper virus production." Molecular and Cellular Biology...
  14. Antibodies 101: Flow Cytometry

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    Blog Post
    ...If this sounds like you, then flow cytometry can help you overcome these challenges and make these types...except one. The fluorescence-minus one panel will help you set up gating during data analysis.  We hope...
  15. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...in healthy and apoptotic cells (9). PA-FPs have helped to revolutionize the field of super resolution ...world of super resolution microscopy, PA-FPs have helped create several new imaging methods, including photoactivation-localization...
Showing: 341 - 360 of 755 results