We narrowed to 763 results for: ELP

... lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using...stable and can withstand ultracentrifugation which helps scientists achieve a high titer (reviewed in Joglekar...

...For us, transient leaf transformations are a great help in screening candidate genes and speeding up research...transformations, but we hope that these tips might help to get you started. Once you have established a ...

... that receive different types of stimulation may help to refine and optimize your stimulation parameters...Happy experimenting and hopefully optogenetics will help illuminate (is that pun worn out yet?) your research...

...are a few simple troubleshooting measures that can help get you back on track: Compatibility: Double-check...inducer or adding an affinity tag such as GST may help with solubility issues. We want to hear about your...

...SlideShare, Figshare and Dataverse. Just as Addgene helps with DNA reagents, these resources can offload the...their data. Likewise, we want to contribute to helping others understand ours. Improving access to data...

... your gel. At their most basic, loading controls help you rule out errors like uneven pipetting or irregular...necessary to demonstrate a POI’s absence. It also helps confirm that lanes are loaded evenly. Created with...

... than 48℃. Both Snapgene and NEB have tools that help you design primers for PCR amplification of fragments...incorporate such regions of homology. This video gives a helpful demonstration of how to use Snapgene’s program ...

...biotinylation reagents to help with the specific elution of biotinylated proteins. For help determining which ...

... probes, we decided to write this quick guide to help researchers choose the probe for their needs. You...assays with other FPs.   We hope that this post helps you in getting to know the state-of-the-art NIR ...

... phage display library with plate-bound antigen helps select for high affinity scFvs. Bound phage are ...expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm (Sandomenico...

...muffled voices beneath masks, humans rely on mouths to help them hear and read emotional cues. We had two options...written and video protocols at Addgene? If you need help in the lab, we have you covered.  ...

...downstream of the 3’ splice acceptor of the transgene to help prevent the accumulation of such truncated proteins...understanding of basic AAV biology, however, would help improve split vector strategies and lead to greater...

...publication, improving your general writing skills will help you in your academic publishing goals and make you...reach out to your peers and other mentors who can help you. You Can Talk to Your Editors I wish I had...

...deposit your piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot...nucleases. Mathew applies his specialized knowledge to help researchers identify the right technologies and ...

...world where so much is out of your hands, it’s helpful to focus on something controllable, like experiments...results of a single mouse. Biological replicates help you to determine which responses arise from drug...

...options to consider, and the following factors will help you determine which one(s) are candidates for your...are a number of optogenetic tools now available to help light your path!   Resources and References Recommended...

...You’ll need to consider if such properties could help, or harm, your experimental goals when designing... other posts in our Viral Vectors 101 series can help guide you with these, so keep reading! Good luck...

...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression... CRISPR has made targeting much easier! With the help of guide RNAs, fusions between Cas proteins and ...

...polybrene (protocol) or DEAE dextran (protocol), which help the viral particle bind to your target cells. If...packaging cell lines to avoid recombination leading to helper virus production." Molecular and Cellular Biology...

...likely won’t work in a given application is just as helpful as knowing when it will. And remember, antibodies...others in the community can benefit and we can all help improve antibody reproducibility!     References...