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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 621 results for: Emb

Showing: 341 - 360 of 621 results

  1. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...the stochasticity of molecular interactions and embedding individual diversity to get from gamete to a visibly...replication and gene transcription during vertebrate embryogenesis. My interests, though, have proven unruly. Instead...

  2. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ... your research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica...also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...

  3. Plasmid Cloning by PCR

    Type
    Blog Post
    ...overhangs to your insert of interest for Gibson assembly. The steps following primer design and the PCR...to ligate our cDNA into the recipient plasmid. Remember to insert your DNA in the correct orientation ...

  4. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...make sure to rinse off the surface of the filter membrane with the surrounding solution to ensure excess...excess viral particles do not remain adhered to the membrane. Be careful when diluting - if you end up concentrating...

  5. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...a CAAX sequence to bind the mRNA to the plasma membrane; this sequence prevents diffusion of the mCherry...SunTag-based system tethers mRNAs to the plasma membrane, it’s not appropriate for use with mRNAs usually...

  7. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...customers, software developers, as well as team members in automation, sales, marketing, and more! This... up. Finish any communications with my UK team members before they log off for the day. 1:45 PM--Write...

  8. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...your network, which includes your friends, family members, and professional network such as co-workers, professors...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page...

  9. Antibodies 101: ChIP

    Type
    Blog Post
    ...that has been validated in multiple assay types. Remember that ChIP uses native proteins (so antibodies ...extra-methodical approach" to the top of your protocols when embarking on your ChIP journey!  References and Resources...

  11. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...now, it’s time to cast aside those concerns and embrace a new resource to solve the problem: CasPEDIA, ...collection for plasmids containing that enzyme, and embedded in the References lists on every page you can ...

  12. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...rmmAkm6PQd — Elizabeth Kenkel (@ElizabethKenkel) December 5, 2018 How social media has helped me in science... researcher’s work day. It’s also important to remember that it’s not the amount of time you spend on ...

  13. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... is important and how it should change how the members of your audience think or act.   To address that...substitute for feedback from others.  Seek out members of your target audience and ask for their feedback...

  17. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this tool? Musunuru...includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available from...

  19. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...between one heavy chain and one light chain. When assembled, the four chains make a Y-shaped protein that ...antib8020028    Wessel GM, McClay DR (1986) Two embryonic, tissue-specific molecules identified by a double-label...

  20. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...and targeting abilities of the other Cas family members make for useful implements in the scientist toolbox...Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular ...
Showing: 341 - 360 of 621 results