We narrowed to 642 results for: Emb;

...laboratory experience is focused on classical embryology techniques such as basic observation and dissections... that will bind to complementary mRNA within an embryo or tissue. Once the riboprobes are created, the...

...will be held on Friday 16th and Saturday 17th September respectively. Join us to discuss advocacy efforts...scientists, both young and established, as they embark on or continue their careers in science-related...

...overlooked regulatory fragments of these transcripts. I remember when small RNA was just something we ran off the...hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they are making fairly...

...aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots to use for qPCR...Tips When calculating the titer for your AAV, remember to account for the dilution of the sample for ...

...break), write down some notes on the card so you remember who they are and what you’ve talked about. After... Resources on the Addgene website Become of a member of the Addgene depositor community ...

...to de novo assembly of the unmapped reads, employing our bioinformatics software to assemble the reads ...

...start life as fertilized eggs that develop into embryos and then become larvae. There are three larval ...importantly, this knowledge will stop you from embarking on a fruitless journey of trying to get males ...

...associate with the endogenously expressed SCF complex members, an E3 ubiquitin ligase complex. Finally, when ...Sep;11(9):713-20. doi: https://doi.org/10.1038/nchembio.1869  Nabet B, Roberts JM, Buckley DL, Paulk J...

... adaptable into future magnetogenetic tools.  Embracing science in the open The York Lab operates with...applications across a wide range of topics. He embraces open science, self-publishing directly on his ...

...this case the cell needs to be fixed and the cell membrane permeabilized in order for the antibodies to reach...Parton's Lab (University of Queensland) to observe membrane trafficking events in vivo. Locate your protein...

...development of vertebrate embryos.  The first 3D reconstructions of clear embryos were obtained by using ...

...sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning vector (deposited... allows labs to create accounts in which their members can share protcols, sign-up for shared equipment...

...else you’d like to say to our readers? Patron: Remember plants. Every time you look at your plate, think...Journal. A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner...

...101784 SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T...AICSDP-42 mTagRFPT CAAX domain of K-Ras Plasma Membrane 114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin...

...likely due to quarantining {% video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist..._playlist=True, viral_sharing=False, embed_button=False, autoplay=False, hidden_controls=False, loop=False...

...2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional resources on the Addgene blog...

... derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family, which...

...!). In 1997, Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry...and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the recipe...

...want to know the orientation of the insert. One member of this type of primer pair anneals to a sequence...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about...

...nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin system has been ...genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A...