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We narrowed to 1,038 results for: LAS

Showing: 341 - 360 of 1038 results

  1. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post
    ...fiberoptic cannulas directly implanted in the injection site and connected to a laser. The laser flashes light... about FLEx vectors Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene...molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids at Addgene ...received her PhD in Virology from the University of Glasgow. She then moved to France where she worked as a...

  2. Lab Automation at Addgene

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    Blog Post
    ... scientists share plasmids across the globe, we’re working on ways to send plasmids as DNA as opposed ...fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials. Have you ever wondered... transformation. With our ever growing rate of plasmid deposits (over 100 deposits per month!), manual...Qiagen BIOROBOT Universal Platform and it suits our plasmid prepping needs perfectly. Working with Qiagen, ...used to be a lab assistant, but with increased plasmid demand it was too time consuming to complete this...There is a tube that goes from the needle to the flask with agar in it. The agar sits on a hot plate with...

  3. Streaking for Single Colonies: The Streak Plate Challenge

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    Blog Post
    ...up your or your class’s streak plate skills? Find the chromoprotein expressing plasmids here, and check... Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids at Addgene Check out additional...other species.  When he found the chromoprotein plasmids available from Addgene, he knew it would be a ...hopefully revealing single, isolated colonies in the last quadrant after incubation. The challenge is usually...molecular biology. “What I'd like to do with the plasmids from Addgene is have the students transform them...

  4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post
    ...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9...looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and...Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel.   Adenoviral vectors...description of their experimental protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9...for instance in inherited disorders such as B-thalassemia, Sickle Cells Disease, Duchenne Muscular Dystrophy...nonalcoholic steatohepatitis cancer. In deficiencies of plasma proteins where Adv vector delivery is often used...

  5. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post
    ...Fluorescent Protein Resources Find Other Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources ...for a transcriptional reporter or as a general cytoplasmic label of cells, there's no problem. You're OK...tag with a serious drawback. The standard EGFP plasmid that used to be sold by Clontech and is in a freezer...stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)A dimerizing protein...dramatic. For a membrane POI localized to the endoplasmic reticulum, the normally spiderweb-like pattern...dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing FP is fused...quality control of secretory proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik...

  6. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post
    ...2011). But this requires other lasers than the standard Ti:Sapphire laser. It is also important to finely...Two low-energy photons (typically from the same laser) cooperate to cause a higher-energy electronic transition...two-photon probes when using a standard Ti:Sapphire Laser include: Blue-green fluorescent proteins like EGFP...fluorescent proteins are excited by 750 nm to 760 nm laser light, enabling dual color imaging studies with ...researcher can flank fluorescent proteins in a plasmid with loxP sites such that their expression is turned... the brains of mice chronically infected with Toxoplasma gondii." The Journal of Immunology 182.10 (2009...cells and dendritic cells during infection with Toxoplasma gondii." PLoS Pathog 5.7 (2009): e1000505. PubMed...

  7. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post
    ...Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids to Fluorescent Protein Fusions ...oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that have mastered...the potential rewards could be substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific...Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have...cell membranes, so they are best used either with plasma membrane proteins (such as GPCRs, ion channels,...40-72 Intact Cells Cy3NTAc Poly His  44 35-63 Plasma membrane protein, permeabilized cells  Cy5NTAc...38-70 Intact Cells Cy3NTAc Poly His  63 50-90 Plasma membrane protein, permeabilized cells  Cy5NTAc...

  8. Save Time with Transient Plant Leaf Transformations

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    Blog Post
    ...transformation Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ... the production of cancer therapeutic drugs, vinblastine and vincristine, are regulated in the medicinal...cannot be studied in hairy roots, as functional chloroplasts and leaf specific cell types are missing in ...its function. In nature Agrobacterium contains a plasmid with a T-DNA (transfer-DNA) region. During infection...localization of your protein of interest. Find all plasmids from the Lee-Parsons lab. Test different leaf...

  9. Bioinformatics at Addgene

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    Blog Post
    ...days, we would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while...while more recently we have moved to full plasmid sequencing at our facility using Illumina MiSeq Next Generation...lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing data collection...transform the raw sequencing data into a complete plasmid sequence that would be ready for our QC team to...With our outline of individual steps to automate plasmid sequence assembly, and possible solutions for each...up with our sequencing instrument (hundreds of plasmids each week!), we already planned to use cloud computing...computer. Eventually, our workflow would handle plasmid sequences on a 96-well plate basis, but for rapid...

  10. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

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    Blog Post
    ...suppressed and NES activity is enhanced, leading to cytoplasmic translocation of the reporter. As a result, the...phosphorylation status. The relative ratio of cytoplasmic vs. nuclear fluorescence serves as a proxy for...monitor global kinase activity that occurs in the cytoplasm and/or nucleus, while FRET-based reporters can...because KTRs shuttle between the nucleus and the cytoplasm, nuclear export and import rates can influence...ERK, or a backbone to use for custom KTR design, plasmids are available from Addgene! References Kudo, ...protocols, 13 1, 155-169. PMID: 29266096. Find the KTR plasmids here! Additional Resources on the Addgene Blog...Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors ...

  11. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

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    Blog Post
    ...gRNA-containing plasmids (McDade et al, 2016). Many of the gRNAs contained within these plasmids have been ...scientists’ submissions, either their deposited gRNA plasmids or from submission of a spreadsheet containing... validated gRNAs that are not generated from a plasmid. If you, for example, prefer to deliver your CRISPR...columns are target gene, species, sequence, Addgene plasmid ID (if we have one associated with the gRNA), application...please consider submitting their sequences (and plasmids!) so that this shared resource can continue to... Tools Read Our CRISPR Guide Pages Find CRISPR Plasmids  ...

  12. Supporting Rare Disease Research with BeHeard 2017

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    Blog Post
    ... disease. This year, Addgene will be providing plasmid grants worth $2,000 each to two labs through the...the BeHEARD Award. Read on to learn how plasmids from Addgene will be used to accelerate rare disease ...in C. elegans, Dr. Di Schiavi will use Addgene plasmids to characterize smn1 interactions with other genes... their function, and C. elegans overexpression plasmids to upregulate their expression in specific cells...tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown, or overexpress... Support Rare Disease Research: IFOPA Donating Plasmids to Researchers in Developing Countries with Seeding...Seeding Labs Resources on Addgene.org Find Plasmids for Disease Research ...

  13. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria." Plasmid 51.3 (2004): 246-255. PubMed...exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate off-target mutations...a mutated allele introduced from a conjugative plasmid replaces the chromosomal copy. This is a versatile... These two steps are (Figure 1): The initial plasmid integration step (the so-called “single crossover... an allelic exchange experiment? Find the pTOX plasmids here! Continued development of new allelic exchange...Additional resources on the Addgene blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn...recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our molecular biology...

  14. Five Popular Model Organisms

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    ... eBook Read our plasmids 101 blog series Resources on Addgene.org Browse our plasmid collection pages... resistant to chemotherapy. You can find these plasmids at Addgene. Another advantage of mice as model... pancreas. They first tagged these cells using plasmids. Then using HOTcre, a method that uses heat induction... feeding the worm bacteria expressing specific plasmids (L4440) that contain your gene of interest (Fire...calcium signaling in the entire adult brain, using a plasmid that expresses a genetically encoded calcium sensor...al., 2015). If you are interested in C. elegans plasmids and resources check out Addgene’s Worm Expression...pages Read our molecular biology reference Find plasmids for your research ...

  15. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    Blog Post
    ...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...assays are qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini et al., 2012...measures homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls...advisable to first express the fusion protein from a plasmid-based system to assess whether the chimera is still...18454154. PubMed Central PMCID: PMC2853173. Sheridan, Douglas L., et al. "A new way to rapidly create functional...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...

  16. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ... resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101 Biotinylation Other resources...insoluble proteins, which can be a challenge for classic antibody based affinity purification/mass spectrometry...vivo studies (Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and... specific locations in the cell.   Find BioID2 plasmids here APEX2: A more sensitive ascorbate peroxidase... They named this mutant APEX2.  Find the APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity...allowing for more precise temporal control.  Find plasmids for TurboID and miniturbo Split-BioID: A conditional...streptavidin and separated via flow cytometry.  Find plasmids for EXCELL here! Since BioID was developed in ...

  17. Understanding GPCR Signaling Using TRUPATH

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    Blog Post
    ...are transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate...effects.  Bryan Roth’s lab created a collection of plasmids called TRUPATH to interrogate 14 of the 16 non-visual...TRUPATH be used for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches...effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists and inverse-agonists...drugs to further understand their mechanism. These plasmids are available as a collection, facilitating screening...Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors for your experiments ...

  18. Addgene at the ASM Microbe 2016 Conference

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    ...steps into this field, we’ve curated microbiology plasmids from the repository onto one handy Microbiology... of researchers who weren’t aware of Addgene’s plasmid sharing services, as well as #happydepositors who...these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright and early on Saturday..., it looks like there are plenty more exciting plasmid tools on the horizon for microbiology research ... distribution service! Did you see a must-have plasmid during ASM? Let us know what’s missing in the collection... collection by filling out our Suggest a Plasmid Form. We look forward to helping microbiologists from...Microbiology Resource Page Deposit Your Microbiology Plasmids with Addgene ...

  19. Technique: Probe Phage Genomes for Host Binding Proteins

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    ...pool and extract plasmid DNA directly from the colonies. Transform the resultant plasmid DNA into E. coli... of phages) constitute one of the most popular classes of phage proteins to study. These are highly useful...units, which can be purchased cheaply, are small plastic containers that can easily be attached with a hose...DNA bases.  Ligate and transform your resultant plasmid libraries into chemically competent E. coli and... you’re using a pET vector) and antibiotic for plasmid selection. Remove air bubbles between the membrane...they expressed well, the proteins encoded by the plasmids in the library will remain attached to the membrane...of Expression Vectors Deposit Your Microbiology Plasmids ...

  20. Viral Vectors 101: Inverted Terminal Repeats

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    ... ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV genome, ITRs serve as...gene of interest in a cell or animal model. AAV plasmids contain several important elements, but among ...molecule as the gene that they package. In a transfer plasmid, the transgene is flanked by two ITRs, and the ...genome of AAV in the native virus. In an AAV vector plasmid, they flank the gene of interest. A single ITR ...growth culture and result in a high percentage of plasmid DNA with mutated ITR sequences. Using recombinant-deficient...located immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these...
Showing: 341 - 360 of 1038 results